Expression and clinical significance of visfatin, hsamiR-199a and extracellular signal regulating protein kinase 1/2 in colorectal cancer

LIU Ting; YU Xiu-Wen; LIU De-Shui; XU Chen; LI Xue-Song; RONG Wei

doi:10.16098/j.issn.0529-1356.2021.03.014

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (3) : 418-424. DOI: 10.16098/j.issn.0529-1356.2021.03.014

Expression and clinical significance of visfatin, hsamiR-199a and extracellular signal regulating protein kinase 1/2 in colorectal cancer

LIU Ting¹ YU Xiu-wen¹ LIU De-shui² XU Cheng³ LI Xue-song³ RONG Wei^3*

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Abstract

Objective To investigate the expression and correlation of visfatin, hsa-miR-199a and extracellular regulated protein kinase 1/2 (ERK1/2) in colorectal cancer (CRC) tissues. Methods The expression levels of visfatin mRNA and hsa-miR-199a were detected by Real-time PCR in 64 primary cases of radical resection of colorectal cancer who underwent pathological clinical examination in the clinicopathological examination in our hospital center from January 2017 to December 2017, the protein expression levels of visfatin and ERK1/2 were detected by immunohistochemistry and Western blotting. The relative expression levels of hsa-miR-199a in each group were compared according to the expression levels of visfatin and ERK1/2. Results The levels of visfatin gene and protein in colorectal cancer tissue(observation group, 64 cases) were higher than those in normal colorectal cancer(control group, 64 cases), and the expression was consistent. The colorectal cancer tissue(observation group) expression level of hsa-miR-199a was lower than that of normal colorectal tissue(control group), and visfatin mRNA was significantly negatively correlated. The colorectal cancer tissue(observation group) expression of ERK1/2 was higher than normal colorectal tissue(control group) and visfatin was significantly positively correlated. The relative expression of hsa-miR-199a in the visfatin and ERK1/2 positive group was significantly different from that in the test variables. Conclusion The overexpression of visfatin in colorectal cancer affects the down-regulation of hsa-miR-199a and is related to the ERK signaling pathway.

Key words

Colorectal cancer / Visfatin / MicroRNA-199a / Extracellular regulated protein kinase1/2 / Real-time PCR / Western blotting / Human

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LIU Ting YU Xiu-wen LIU De-shui XU Cheng LI Xue-song RONG Wei. Expression and clinical significance of visfatin, hsamiR-199a and extracellular signal regulating protein kinase 1/2 in colorectal cancer[J]. Acta Anatomica Sinica. 2021, 52(3): 418-424 https://doi.org/10.16098/j.issn.0529-1356.2021.03.014

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