Effects of microRNA-4286 on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ

LIU Dong-Tao

doi:10.16098/j.issn.0529-1356.2021.03.012

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (3) : 405-409. DOI: 10.16098/j.issn.0529-1356.2021.03.012

Effects of microRNA-4286 on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ

LIU Dong-tao YANG Zhi-juan DING Bo MA Jun-wen*

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Abstract

Objective To investigate the effects of microRNA-4286 (miRNA-4286) on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ (INPP4A). Methods HGC-27 cells were divided into blank control group, negative control group and intervention group. The blank control group received no treatment, negative control group was transfected with pEGFP-N1 plasmid, and intervention group was transfected with pEGFP-N1-miRNA-4286 plasmid. Real-time PCR was used to detect the expression of miRNA-4286 in HGC-27 cells, and Western blotting was used to detect the expression of INPP4A protein in HGC-27 cells. The proliferation rate, invasion ability and migration ability of HGC-27 cells were detected by cholecystokinin octapeptide, cholecystokinin octapeptide(CCK8), Transwell chamber assay and scratch test, respectively. Results The miRNA-4286 expression level, proliferation rate, and number of transmembrane cells in intervention group were significantly higher than those in blank control group and negative control group (P<0.05). The expression level of INPP4A protein and the scratch healing ability of intervention group were significantly lower than those of blank control group and negative control group (P<0.05). The miRNA-4286 expression, INPP4A protein expression, proliferation rate, number of transmembrane cells, and the scratch healing ability of negative control group had no significant difference with those of blank control group (P>0.05). Conclusion miRNA-4286 may promote the growth, migration and invasion of gastric cancer cell line HGC-27 by down-regulating the expression of INPP4A.

Key words

MicroRNA-4286 / Inositol polyphosphate-4-phosphatase typeⅠ / Gastric cancer / HGC-27 cell / Cell invasion / Cell migration / Western blotting

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LIU Dong-tao YANG Zhi-juan DING Bo MA Jun-wen. Effects of microRNA-4286 on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ[J]. Acta Anatomica Sinica. 2021, 52(3): 405-409 https://doi.org/10.16098/j.issn.0529-1356.2021.03.012

References

［1］ Feng P, Fan WJ, Liu L, et al. Inhibitory effect of recombinant human semaphorin 3A on angiogenesis of gastric cancer and the associated mechanisms［J］. Acta Anatomica Sinica, 2020,51(2):11-13. (in Chinese)

冯品, 范文静, 刘镭,等. 重组人信号素3A抑制胃癌血管生成及其机制［J］. 解剖学报, 2020,51(2):11-13.

［2］ Cai R, Zhu Y, Wang KF, et al. Effect of melatonin on the expression of Th1/Th2/Th17 cytokines of gastric cancer in vitro and in vivo［J］. Acta Anatomica Sinica, 2019, 50(4):471-476. (in Chinese)

蔡蓉, 朱瑜, 王开放,等. 褪黑素体内外对胃癌Th1/Th2/Th17型细胞因子表达的影响［J］. 解剖学报, 2019, 50(4):471-476.

［3］ Ji L, Selleck MJ, Morgan JW,et al.Gastric cancer peritoneal carcinomatosis risk score［J］. Ann Surg Oncol, 2020, 27(1): 240-247.

［4］ Lena F, Sarah TH, Daniel S, et al. MicroRNA profiles of MS grary matter lesions identify modulators of the synaptic protein synaptotagmin-7［J］. Brain Pathol, 2020, 30(3): 524-540.

［5］ Banihashemi S, Tahmasebi-Birgani M, Mohammadiasl J, et al. Whole exome sequencing identified a novel nonsense INPP4A mutation in a family with intellectual disability［J］. Eur J Med Genet, 2020, 63(4):103846-103850.

［6］ Aki S, Yoshioka K, Takuwa N, et al. TGFβ receptor endocytosis and Smad signaling require synaptojanin1, PI3K-C2α, and INPP4Bmediated phosphoinositide conversions［J］. Mol Biol Cell, 2020, 31(5):360-372.

［7］ Guo HY, Gao Y, Zhang ChJ, et al. Correlation between serum miR-4286 expression and clinicopathological characteristics of gastric cancer and its clinical significance［J］. China Journal of Modern Medicine, 2019, 29(10):33-38. (in Chinese)

郭红艳, 高勇, 张春晶, 等. 血清microRNA-4286表达与胃癌临床病理特征的关系及其临床意义［J］. 中国现代医学杂志, 2019, 29(10):33-38.

［8］ Zhang M, Tian H, Li R, et al. MicroRNA-4286 promotes esophageal carcinoma development by targeting INPP4A to evoke the JAK2/STAT3 pathway activation［J］. Pharmazie,2018,73(6):342-348.

［9］ Chen X, Yu XT, Shen E. Overexpression of CDKN2B is involved in poor gastric cancer prognosis［J］. J Cell Biochem, 2019,120(12):19825-19831.

［10］ Lederer WH, Gerstbrein HL. Creatine kinase isoenzyme BB activity in serum of a patient with gastric cancer［J］. Clin Chem,1976,22(10）:1748-1749.

［11］ Witwer KW. Circulating microRNA biomarker studies: pitfalls and potential solutions［J］. Clin Chem,2015,61(1):56-63.

［12］ Hoon BJ, Qing Z, Feng L, et al. The landscape of microRNA, Piwi-interacting RNA, and circular RNA in human saliva［J］. Clin Chem,2015,61(1):221-230.

［13］ Lena F, Sarah TH, Daniel S, et al. MicroRNA profiles of MS gray matter lesions identify modulators of the synaptic protein synaptotagmin-7［J］. Brain pathol, 2020,30(3):524-540.

［14］ Ling C, Wang X, Zhu J, et al. MicroRNA-4286 promotes cell proliferation, migration, and invasion via PTEN regulation of the PI3K/Akt pathway in non-small cell lung cancer［J］. Cancer Med, 2019, 8(7):3520-3531.

［15］ Anna K, Nadezhda P, Mariya A, et al. Antiproliferative and Pro-apoptotic effects of miR-4286 inhibition in melanoma cells［J］. PLoS One, 2016, 11(12):e0168229.

［16］ Sung JY, Na K, Kim HS. Down-regulation of inositol polyphosphate 4-phosphatase type Ⅱ expression in colorectal carcinoma［J］. Anticancer Res, 2017, 37(10):5525-5531.

［17］ Wang H, Loerke D, Bruns C, et al. Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway［J］. J Biol Chem, 2020, 295(4):1091-1104.