Screening and identification the hub genes of cardiac hypertrophy in mice

SHI Zhuo; LI Jia-Hui; GAO Jing; DONG Yan-Li; REN Fu; WEN Pu-Shuai

doi:10.16098/j.issn.0529-1356.2021.03.010

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (3) : 391-397. DOI: 10.16098/j.issn.0529-1356.2021.03.010

Cell and Molecules Biology

Screening and identification the hub genes of cardiac hypertrophy in mice

SHI Zhuo^1,2,3 LI Jia-hui⁴ GAO Jing⁵ DONG Yan-li⁶ REN Fu^1,2* WEN Pu-shuai^2,7*

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Abstract

Objective To screen and identify the hub genes closely related to cardiac hypertrophy by using bioinformaticsmethod and biological experiments. Methods The chip data related to cardiac hypertrophy in mice were downloaded from the Gene Expression Omnibus(GEO) database, and the GEO2R online tool was adopted to screen for differentially expressed genes; DAVID 6.7, String 11.0 and Cytoscape 3.7.0 softwares were used to analyze differentially expressed genes; Kunming mice were randomly divided into a normal saline group (n=6) and an angiotensin Ⅱ (Ang Ⅱ) group (n=6) to establish a cardiac hypertrophy model, the expression of hub genes in Kunming mouse model of cardiac hypertrophy induced by AngⅡ was detected by Real-time PCR method . Results A total of 202 common differentially expressed genes and 12 hub genes were selected; the Real-time PCR result demonstrated that decorin(Dcn), HADHA and heat shock protein(HSP)90αA1 were significantly down-regulated in the AngⅡ group. Conclusion The selected hub genes can influence the development of cardiac hypertrophy in Kunming mice through extracellular matrix and transforming growth factor β(TGF-β).

Key words

Cardiac hypertrophy / Bioinformatics / Differentially expressed gene / Real-time PCR / Kunming mouse

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SHI Zhuo LI Jia-hui GAO Jing DONG Yan-li REN Fu WEN Pu-shuai. Screening and identification the hub genes of cardiac hypertrophy in mice[J]. Acta Anatomica Sinica. 2021, 52(3): 391-397 https://doi.org/10.16098/j.issn.0529-1356.2021.03.010

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