Effects of up-regulation of zinc finger and BTB domain containing 20 expression on learning and memory of APPswe/PSE9 transgenic Alzheimer disease mice

WANG Yue-Jing; SUN Li-Hui

doi:10.16098/j.issn.0529-1356.2021.03.006

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (3) : 365-369. DOI: 10.16098/j.issn.0529-1356.2021.03.006

Effects of up-regulation of zinc finger and BTB domain containing 20 expression on learning and memory of APPswe/PSE9 transgenic Alzheimer disease mice

WANG Yue-jing¹ ZHANG Meng¹ LANG Wei-ya¹ ZHANG Hai-yan¹LIAN Jie¹ FENG Hua-jie² SUN Li-hui^1*

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Abstract

Objective To investigate the effects of up-regulation of zinc finger and BTB domain containing 20（A20）expression on learning and memory and apoptosis of hippocampal neurons in APPswe/PSE9 transgenic Alzheimer disease（AD）mice. Methods Forty APPswe/PSE9 transgenic mice were divided into four groups: control group, AD group, small interfering RNA(siRNA）-AD group and A20-AD group. Adenoviruses carrying high expression of A20 were selected as interfering drugs and Morris water maze was used to observe spatial learning and memory. Western blotting was used to detect the expression of A20. Immunohistochemistry was used to observe the expression of P53 in the CA1 region. Results Compared with the AD group, the learning and memory function was mproved, the apoptosis rate of hippocampal CA1 neurons decreased significantly and the expression of P53 in the CA1 region decreased significantly in A20-AD group mice. Conclusion Upregulation of A20 expression can improve the learning and memory ability of transgenic mice, reduce the expression of P53 in hippocampal CA1 region of the transgenic mice.

Key words

Zinc finger and BTB domain containing 20 / Dementia / Immunohistochemistry / Water maze test / Mouse

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WANG Yue-jing ZHANG Meng LANG Wei-ya ZHANG Hai-yan LIAN Jie FENG Hua-jie SUN Li-hui. Effects of up-regulation of zinc finger and BTB domain containing 20 expression on learning and memory of APPswe/PSE9 transgenic Alzheimer disease mice[J]. Acta Anatomica Sinica. 2021, 52(3): 365-369 https://doi.org/10.16098/j.issn.0529-1356.2021.03.006

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