&nbsp;Acupoint catgut embedding inhibiting neuroinflammation in vascular dementia rats

ZHU Shi-Jie; HU Xue-Mei; TANG Zhong-Sheng; XIE Gao-Yu; WU Chun-Peng; KOU Yun-Fang; LUO Ya-Fei

doi:10.16098/j.issn.0529-1356.2021.03.002

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (3) : 337-343. DOI: 10.16098/j.issn.0529-1356.2021.03.002

Neurobiology

Acupoint catgut embedding inhibiting neuroinflammation in vascular dementia rats

ZHU Shi-jie¹ SUN Qiu-ying²TANG Zhong-sheng^1* LUO Ya-fei¹ XIE Gao-yu¹ WU Chun-peng¹ KOU Yun-fang¹ FAN Rui-juan¹

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Abstract

Objective To observe the effect of acupoint catgut embedding on the expression of inflammatory factor mRNA in cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2) signal pathway of vascular dementia (VD) rats, and to explore the protective mechanism of acupoint catgut embedding on the brain inflammatory response of VD rats. Methods VD model was established by the modified Pulsinelli’s four vessel blocking method . Totally 148 male rats were randomly divided into VD model group, non acupoint catgut embedding group and acupoint catgut embedding group. On the 7th day after operation, catgut embedding at acupoints and catgut embedding at non acupoints were performed in the two treatment groups respectively, and materials were taken out 15 days later. Western blotting was used to detect the expression of COX-2 and PGE2, and real-time PCR was used to detect the mRNA expression of tumor necrosis factor α(TNF-α), intercellular cell adhesion molecule 1 (ICAM-1), interleukin(IL)-6, macrophage inflammatory protein 2 (MIP-2), IL-1β, and monocyte chemotactic protein 1 (MCP-1) in rat hippocampus. Results Compared with the sham group, the expressions of COX-2, PGE2, TNF-α, ICAM-1, IL-6, MIP-2, IL-1β and MCP-1 in hippocampus of the other three groups were significantly higher (P<0.01). Compared with the model group, the expressions of COX-2, PGE2 protein and TNF-α, ICAM-1, IL-6, MIP-2, IL-1β, MCP-1 mRNA in the hippocampus of the acupoint catgut embedding group and the non acupoint catgut embedding group decreased significantly (P<0.01). Conclusion Acupoint catgut embedding can protect the brain from inflammatory injury by down-regulating the expression of related inflammatory factors in COX-2/PGE2 signaling pathway and reducing the inflammatory response induced by VD rats.

Key words

Acupoint catgut embedding / Vascular dementia / Inflammatory factor / Western blotting / Rat

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ZHU Shi-jie SUN Qiu-ying TANG Zhong-sheng LUO Ya-fei XIE Gao-yu WU Chun-peng KOU Yun-fang FAN Rui-juan. Acupoint catgut embedding inhibiting neuroinflammation in vascular dementia rats[J]. Acta Anatomica Sinica. 2021, 52(3): 337-343 https://doi.org/10.16098/j.issn.0529-1356.2021.03.002

References

[1］ Zhang XSh, Sun JN, Guo J, et al. Study on the effect of total alkaloids of Peganum harmala on learning and memory of vascular dementia rats［J］.Pharmacology and Clinic of Traditional Chinese Medicine,2017, 44（8）:75-78.（in Chinese）

张晓双，孙建宁，郭洁，等.骆驼蓬总碱对血管性痴呆大鼠学习记忆的研究［J］.中药药理与临床，2017，44（8）：75-78.

[2］ Tang ZhSh, Li X, Wu ChP, et al. Effect of acupoint catgut embedding on hemorheology and expression of p75NTR in hippocampal CA1 neurons of vascular dementia rats［J］. Journal of Practical Medicine,2017,（16）:2628-2631.（in Chinese）

唐中生，李霞，吴春朋，等.穴位埋线对血管性痴呆大鼠血液流变学和海马CA1区神经元p75NTR表达的影响［J］.实用医学杂志，2017，（16）：2628-2631.

[3］ Cui J, Chen PB, Yang XF, et al. Effect of the simple acupoint catgut embedding on the expression of ICAM-1, NF-kappaB and airway inflammation in rats with asthma［J］. Anesth Analg, 2017, 125(2):662-669.

［4］ Fang YN, Sui RB, Zhang L. Effect of acupuncture on inflammatory cytokines in hippocampus of vascular dementia rats ［J］.Journal of Xi’an Jiaotong University (Medical Edition), 2016, 37（4）:586-589.（in Chinese）

房雅楠，隋汝波，张磊.针刺完骨穴对血管性痴呆大鼠海马炎性细胞因子的影响［J］.西安交通大学学报（医学版），2016，37（4）：586-589.

［5］ Gr?sch S, Niederberger E, Geisslinger G.Investigational drugs targeting the prostaglandin E2 signaling pathway for the treatment of inflammatory pain［J］.Expert Opin Investig Drugs, 2017, 26(1):51-61.

［6］ Li WD, Jiang HB, Lian XQ, et al. Improvement of the method of making brain ischemia model by Pulsinelli four vessel method in rats［J］.Journal of Xinxiang Medical College,2010,27(3):237-239.（in Chinese）

李文东，姜洪波，连晓倩，等.Pulsinelli四血管法大鼠全脑缺血模型制作方法的改进［J］.新乡医学院学报，2010,27(3):237-239.

［7］ Li ZhR. Experimental Acupuncture Science［M］. BeiJing: China Press of Traditional Chinese Medicine,2003:184-185.（in Chinese）

李忠仁.实验针灸学［M］.北京：中国中医药出版社，2003:184-185.

［8］ Yang ChH, Wang YY. Pathogenesis and treatment of vascular dementia in traditional chinese medicine［J］.Traditional Chinese Medicinal Research,2005，18(5):6-7.（in Chinese）

杨辰华，王永炎.血管性痴呆的中医病机及辨治思路［J］.中医研究，2005，18(5):6-7.

［9］ Li HB, Tan ZH. Study on neurovascular unit of vascular dementia based on collateral disease theory［J］.Lishizhen Medicine and Materia Medica Research,2016,27（10）：2481-2483.（in Chinese）

李红兵，谭子虎.基于络病理论对血管性痴呆神经血管单元的研究［J］.时珍国医国药，2016,27（10）：2481-2483.

［10］ Yang ChH, Wang YY, Wang XZh. Syndrome elements and pathogenesis of vascular dementia［J］.Journal of Beijing University of Traditional Chinese Medicine,2006,29（10）：665-667.（in Chinese）

杨辰华，王永炎，王新志.血管性痴呆的证候要素与玄府病机［J］.北京中医药大学学报，2006,29（10）：665-667.

［11］ Kalaria RN.Neuropathological diagnosis of vascular cognitive impairment and vascular dementia with implications for Alzheimer’s disease［J］.Acta Neuropathol, 2016, 131 (5): 659-685.

［12］ Wang J, Chen Y, Liang H, et al. The role of disturbed small-world networks in patients with white matter lesions and cognitive impairment revealed by resting state function magnetic resonance images (rs-fMRI)［J］.Med Sci Monit, 2019, 25:341-356.

［13］ Lin CC, Hsieh HL, Liu SW, et al. BK induces cPLA2 expression via an autocrine loop involving COX-2-derived PGE2 in rat brain astrocytes［J］. Mol Neurobiol, 2015, 51(3):1103-1115.

［14］ Yang Y, Chen Q, Zhao Q, et al. Inhibition of COX-2/PGD2-related autophagy is involved in the mechanism of brain injury in T2DM rat［J］.Front Cell Neurosci, 2019, 13:68.

［15］ Shiow LR, Favrais G, Schirmer L,et al. Reactive astrocyte COX2-PGE2 production inhibits oligodendrocyte maturation in neonatal white matter injury［J］.Glia, 2017, 65(12):2024-2037.

［16］ Song Q, Fan C, Wang P, et al. Hippocampal CA1 βCaMKⅡ mediates neuroinflammatory responses via COX-2/PGE2 signaling pathways in depression［J］.J Neuroinflamm, 2018,15（1）:338.

［17］ Echizen K, Hirose O, Maeda Y,et al. Inflammation in gastric cancer: interplay of the COX-2/prostaglandin E2 and Toll-like receptor/MyD88 pathways［J］.Cancer Sci, 2016,107 (4):391-397.

［18］ Huang X, Pan Q, Mao Z,et al. Sinapic acid inhibits the IL-1β-induced inflammation via MAPK downregulation in rat chondrocytes［J］.Inflammation, 2018, 41(2):562-568.

［19］ Damlar Ⅰ, Esen E, Tatli U.Effects of glucosamine-chondroitin combination on synovial fluid IL-1β, IL-6, TNF-α and PGE2 levels in internal derangements of temporomandibular joint［J］. Med Oral Patol Oral Cir Bucal, 2015, 20(3):e278-283.

［20］ da Silva JB, Carvalho E, Covarrubias AE,et al. Induction of TNF-alfa and CXCL-2 mRNAs in different organs of mice infected with pathogenic Leptospira ［J］.Microb Pathog, 2012,52(4):206-216.

［21］ Liu CW, Sung HC,Lin SR,et al. Resveratrol attenuates ICAM-1 expression and monocyte adhesiveness to TNF-α-treated endothelial cells: evidence for an anti-inflammatory cascade mediated by the miR-221/222/AMPK/p38/NF-κB pathway［J］.Sci Rep, 2017,7:44689.

［22］ Dohgu S, Takata F, Matsumoto J,et al. Monomeric α-synuclein induces blood-brain barrier dysfunction through activated brain pericytes releasing inflammatory mediators in vitro［J］. Microvasc Res, 2019,124:61-66.