High protein acetylation/succinylation levels and their correlation with high histone 2AX expression level in breast cancer

GAO Xiu-Li; YUE Li-Ling; ZHAO Yue-Sheng; LIU Li-Kun; LIU De-Shui; ZHU Wen-Bin; ZHOU Li

doi:10.16098/j.issn.0529-1356.2021.02.013

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (2) : 244-250. DOI: 10.16098/j.issn.0529-1356.2021.02.013

High protein acetylation/succinylation levels and their correlation with high histone 2AX expression level in breast cancer

GAO Xiu-li¹ YUE Li-ling^1*ZHAO Yue-sheng² LIU Li-kun¹ LIU De-shui¹ ZHU Wen-bin¹ ZHOU Li¹#br#

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Abstract

Objective To explore the protein acetylation/succinylation and histone 2AX(H2AX) expression levels in breast cancer, as well as their correlation. Methods By Western blotting and RT-PCR methods to detect the protein modification and H2AX expression levels in 11 breast cancer tissues and cells, as well as to explore the common regulation way of protein acetylation and succinylation by treatment of histone deacetylase inhibitors; To study the relationship between H2AX expression level and protein modification level through the construction and over-expression of indicated plasmids. Results Compared with the adjacent normal tissues, there existed an increase protein acetylation/succinylation levels in breast cancer tissues, and the protein acetylation and succinylation were both regulated by histone deacetylase(HDAC) members. The H2AX mRNA and protein expression levels were increased both in breast cancer cell and tissues, its expression level and the expression and modification level of represented protein nucleophosmin 1(NPM1) showed a positive correlation. Conclusion The breast cancer possesses a characteristic of high protein acetylation/succinylation levels and high H2AX expression level, the H2AX expression level and the modification level of partial proteins in breast cancer have a positive correlation.

Key words

Breast cancer / Acetylation / Succinylation / Histone 2AX / Nucleophosmin 1 / Western blotting / RT-PCR / / Human

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GAO Xiu-li YUE Li-ling ZHAO Yue-sheng LIU Li-kun LIU De-shui ZHU Wen-bin ZHOU Li. High protein acetylation/succinylation levels and their correlation with high histone 2AX expression level in breast cancer[J]. Acta Anatomica Sinica. 2021, 52(2): 244-250 https://doi.org/10.16098/j.issn.0529-1356.2021.02.013

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