Targeted metabolomics analysis of amino acid during rat liver regeneration

YANG Hui; XU Cun-Shuan

doi:10.16098/j.issn.0529-1356.2021.02.008

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (2) : 210-215. DOI: 10.16098/j.issn.0529-1356.2021.02.008

Cell and Molecules Biology

Targeted metabolomics analysis of amino acid during rat liver regeneration

YANG Hui^{1, 2} XU Cun-shuan^{1, 2*}

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Abstract

Objective To explore the regulation of amino acid metabolism during rat liver regeneration (LR). Methods Rats were randomly divided into 10 groups with 5 rats in each group, including nine partial hepatectomy (PH) groups and one normal control group. Selective reaction monitoring/multi reaction monitoring(SRM/MRM) was employed in the targeted metabolomics identification of 20 amino acids at 10 time points in rat liver regeneration. The change of amino acid content was analyzed by hierarchical clustering. Results Alanine was up-regulated at 30, 36 and 72 hours; arginine was up-regulated at 6 and 12 hours; aspartic acid was up-regulated at 6, 12 and 36 hours; glutamate was up-regulated at 6, 12, 30, 36, 72 and 120 hours; histidine was up-regulated at 12, 24, 30, 36, 72 and 120 hours; glutamine was up-regulated at 72 hours, and isoleucine was down-regulated at 24 hours. Through hierarchical clustering analysis of amino acids，these amino acids can be grouped into three clusters. Conclusion Many amino acids have changed during the liver regeneration, and throughout the whole process of rat liver regeneration. Changes in amino acids content play an important role in hepatocyte proliferation during the liver regeneration.

Key words

Liver regeneration / Amino acids / Targeted metabolomics / Hierarchical clustering / Rat

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YANG Hui XU Cun-shuan. Targeted metabolomics analysis of amino acid during rat liver regeneration[J]. Acta Anatomica Sinica. 2021, 52(2): 210-215 https://doi.org/10.16098/j.issn.0529-1356.2021.02.008

References

［1］ Zang XY, Geng XF, Zhang ChY, et al. Role of microRNAs in liver regeneration ［J］. Acta Anatomica Sinica, 2017, 48 (2): 230-235. (in Chinese)

臧夏炎, 耿小芳, 张春艳, 等. MicroRNAs在肝再生中的作用研究进展［J］. 解剖学报, 2017, 48 (2): 230-235.

［2］ Xing XK, Li MH, Xu CSh. Expression changes of AKT,ATM,D4-GDI and p53 cell apoptosis pathway genes in the process of rat liver regeneration ［J］. Acta Anatomica Sinica, 2016, 47 (3): 315-322. (in Chinese)

邢雪琨, 李梦华, 徐存拴. AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在大鼠肝再生过程中的表达变化［J］. 解剖学报, 2016, 47 (3): 315-322.

［3］ Chou KC. Prediction of tight turns and their types in proteins ［J］. Anal Biochem, 2000, 286(1):1-16.

［4］ Chou KC. Modelling extracellular domains of GABA-A receptors: subtypes 1, 2, 3, and 5 ［J］. Biochem Biophys Res Commun, 2004, 316(3): 636-642.

［5］ Chou KC, Shen HB. Signal-CF: a subsite-coupled and window-fusing approach for predicting signal peptides ［J］. Biochem Biophys Rese Commun, 2007, 357(3): 633-640.

［6］ Xu C, Chen X, Chang C, et al. Transcriptome analysis of hepatocytes after partial hepatectomy in rats ［J］. Deve Genes Evol, 2010, 220(9-10): 263-274.

［7］ Sonntag D, Scandurra FM, Friedrich T, et al. Targeted metabolomics for bioprocessing［J］. BMC Proc, 2011, 5(Suppl 8): 27.

［8］ Ito K, Ozasa H, Noda Y, et al. Effect of non-essential amino acid glycine administration on the liver regeneration of partially hepatectomized rats with hepatic ischemia/reperfusion injury［J］. Clin Nutr, 2008, 27(5): 773-780.

［9］ Yuan Y, Wu X, Ou Q, et al. Differential expression of the genes involved in amino acids and nitrogen metabolisms during liver regeneration of mice ［J］. Hepatology Res, 2009, 39(3): 301-312.

［10］ Chiba T, Kawai K, Ikenaka K, et al. Effect of an exogenous energy source and amino acids on DNA synthesis in regenerating rat liver ［J］. Biochim Biophysica Acta, 1983, 755(3): 420-427.

［11］ Mead JE, Braun L, Martin DA, et al. Induction of replicative competence (“priming”) in normal liver ［J］. Cancer Res, 1990, 50(21): 7023-7030.

［12］ Nelsen CJ, Rickheim DG, Tucker MM, et al. Amino acids regulate hepatocyte proliferation through modulation of cyclin D1 expression ［J］. J Biol Chem, 2003, 278(28): 25853-25858.

［13］ Holecek M, Vodenicarovova M. Phenylbutyrate exerts adverse effects on liver regeneration and amino acid concentrations in partially hepatectomized rats ［J］. Int J Exp Pathol, 2016, 97(3): 278-284.

［14］ Yin L, Chang C, Xu C. Expressions profiles of the proteins associated with carbohydrate metabolism in rat liver regeneration ［J］. Biomed Rese Int, 2017, 2017(7): 8428926.

［15］ Tanaka T, Ando M, Yamashita T, et al. Effects of alanine and glutamine administration on the inhibition of liver regeneration by acute ethanol treatment ［J］. Alcohol Alcohol Suppl, 1993, 1B: 41-45.

［16］ Yoshida S, Yunoki T, Aoyagi K, et al. Effect of glutamine supplement and hepatectomy on DNA and protein synthesis in the remnant liver ［J］. J Surg Res, 1995, 59(4): 475-481.

［17］ Schmitt B, Vicenzi M, Garrel C, et al. Effects of N-acetylcysteine, oral glutathione (GSH) and a novel sublingual form of GSH on oxidative stress markers: a comparative crossover study ［J］. Redox Biol, 2015, 6(12):198-205.

［18］ Kurokawa T, An J, Tsunekawa K, et al. Effect of L-arginine supplement on liver regeneration after partial hepatectomy in rats ［J］. World J Surg Oncology, 2012, 10:99.

［19］ Chang CF, Fan JY, Zhang FC, et al. Transcriptome atlas of eight liver cell types uncovers effects of histidine catabolites on rat liver regeneration ［J］. J Genet, 2010, 89(4): 425-436.

［20］ Patel D, Menon D, Bernfeld E, et al. Aspartate rescues S-phase arrest caused by suppression of glutamine utilization in KRas-driven cancer cells ［J］. J Biol Chem, 2016, 291(17): 9322-9329.

［21］ Spolter PD, Harper AE. Effect of leucine-isoleucine and valine antagonism and comparison with the effect of ethionine on rat liver regeneration ［J］. Arch Biochem Biophys, 1963, 100(3):369-377.