Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons

YIN Hai-Yan; WANG Ze-Yu; HAO Chang-Jiang; ZHANG Hui; WANG Feng-Qin; LI Xiu-Guo; CHEN Jing

doi:10.16098/j.issn.0529-1356.2021.02.003

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (2) : 175-181. DOI: 10.16098/j.issn.0529-1356.2021.02.003

Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons

YIN Hai-yan¹ WANG Ze-yu¹ HAO Chang-jiang¹ ZHANG Hui¹ WANG Feng-qin¹ LI Xiu-guo^2* CHEN Jing^3*

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Abstract

Objective To study the temporal and spatial expressions of G protein-coupled receptor, putative receptor protein related to angiotensin type 1 receptor (APJ), in mammal cochlea postnatal development. Methods The cochlear tissues of each group 11 C57BL/6 mice at postnatal day 7 (P7), P14, P28 and postnatal month 2(P2M) were taken out under a stereo microscope. Real-time PCR, Western blotting and immunofluorescent staining were used to detect the expressions of APJ in hair cells and spiral ganglion neurons. Results The expression pattern of APJ in cochleae showed an upward trend during the period from P7 to P2M. The temporal expressions of APJ in hair cells and spiral ganglion neurons increased obviously at P14 and P2M. The spatial expression patterns of APJ in hair cells and spiral ganglion neurons followed a declined gradient from base turns to apex turns at P14. Conclusion APJ expression exhibits a specific spatial and temporal pattern during mouse cochlea postnatal development, and may play a role in cochleae maturation and hearing formation.

Key words

Putative receptor protein related to angiotensin type 1 receptor / Hair cell / Spiral ganglion neuron / Immunofluorescence / Mouse

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YIN Hai-yan WANG Ze-yu HAO Chang-jiang ZHANG Hui WANG Feng-qin LI Xiu-guo CHEN Jing. Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons[J]. Acta Anatomica Sinica. 2021, 52(2): 175-181 https://doi.org/10.16098/j.issn.0529-1356.2021.02.003

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