&nbsp;Transforming growth factor β signaling pathway regulating the function of follicle stimulating hormone in ovarian granulosa cells

WANG Yu-Tong; WEI Xiao-Hong; GE Ling; YANG Yun-Feng; XU Jian

doi:10.16098/j.issn.0529-1356.2021.01.019

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (1) : 118-123. DOI: 10.16098/j.issn.0529-1356.2021.01.019

Histology,Embryology and Developmental Biology

Transforming growth factor β signaling pathway regulating the function of follicle stimulating hormone in ovarian granulosa cells

WANG Yu-tong WEI Xiao-hong GE Ling YANG Yun-feng XU Jian*

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Abstract

Objective To explore the interaction between follicle stimulating hormone (FSH) and transforming growth factor Beta (TGF-β) signaling pathway in rat ovarian granulosa cells. Methods The granulosa cells isolated from the follicles of 21 days SD rats. The experiments were divided into three groups: control group, FSH treated group and transforming growth factor beta receptor Ⅱ (TGF-β RⅡ) neutralizated group. Immunocytochemistry (ICC) and Western blotting were then used to locate and detect the expression level of TGF-β RⅡ and proliferating cell nuclear antigen (PCNA). The proliferation index (PI), cell cycle and percentage of apoptotic cells were assessed by flow cytometry (FCM), and the level of estradiol (E2) was determined by ELISA. Results FSH increased the expression of PCNA and PI, changed the cell cycle and inhibited apoptosis of GCs, and these actions were reduced significantly when TGF-βsignaling pathway was inhibited(P<0.05); FSH stimulated the secretion of E2, and the increase in E2 levels was not regulated by inhibition of the TGF-β signaling pathway(P>0.05). Conclusion The effects of FSH on ovarian granulosa cells are partly affected by the TGF-β signaling pathway.

Key words

Granulosa cells culture / Follicle stimulating hormone / Transforming growth factor β / Immunohistochemistry / Western blotting / Rat

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WANG Yu-tong WEI Xiao-hong GE Ling YANG Yun-feng XU Jian. Transforming growth factor β signaling pathway regulating the function of follicle stimulating hormone in ovarian granulosa cells[J]. Acta Anatomica Sinica. 2021, 52(1): 118-123 https://doi.org/10.16098/j.issn.0529-1356.2021.01.019

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