Expression and mechanism of microRNA-27a in cervical cancer

ZHANG Hong-Ping; LI Feng; NIU Zhan-Jie

doi:10.16098/j.issn.0529-1356.2021.01.012

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (1) : 78-83. DOI: 10.16098/j.issn.0529-1356.2021.01.012

Cancer Biology

Expression and mechanism of microRNA-27a in cervical cancer

ZHANG Hong-ping¹ LI Feng^1* NIU Zhan-jie²

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Abstract

Objective To investigate the effects of microRNA (miR)-27a on proliferation, apoptosis and invasion of cervical cancer cells by targeting F-box and WD repeat domain containing protein 7( FBXW7) expression. Methods Thirty cases of cervical cancer and paracancerous tissues and 30 cases of normal tissues were used in the experiment. The expression of miR-27a in cervical cancer, paracancerous tissue, normal cervical tissue, cervical cancer cells (SiHa, Caski, HeLa, HCC94) and cervical squamous epithelial immortalized cell H8 were detected by Real-time PCR. MiR-27a inhibitor and its negative control were transfected into SiHa cells by liposome transfection. CCK-8 assay, flow cytometry and Transwell assay were used to detect the effects of miR-27a on the proliferation, cell cycle, apoptotic rate and invasive ability of SiHa cells.Bioinformatics was used to predict the targeting gene of miR-27a. Double luciferase reporter gene assay combined with Western blotting was used to verify the targeting regulation of miR-27a on FBXW7. Results Compared with the normal cervical tissues and the adjacent tissues, the expression of miR-27a was higher in cervical cancer tissues (P<0.05); Compared with the cervical squamous epithelial immortalized cells H8, the expression of miR-27a in cervical cancer cells SiHa, Caski, HeLa and HCC94 was higher (P<0.05). Inhibiting the expression of miR-27a in SiHa cells could significantly reduce the proliferation activity of cells (P<0.05), increase the proportion of G0/G1 cells (P<0.05), decrease the proportion of G2/M cells (P<0.05), increase the apoptosis rate (P<0.05), and inhibit the invasive ability of cells (P<0.05). Bioinformatics predicted that FBXW7 might be a target regulatory gene of miR-27a. Double luciferase reporter gene assay showed that miR-27a could specifically bind to the 3’UTR region of FBXW7 (P<0.05), and negatively regulate the expression of FBXW7 protein (P<0.05). Conclusion MiR-27a plays an oncogene role in the occurrence and development of cervical cancer. Inhibiting the expression of miR-27a can significantly inhibit the malignant biological behavior of cervical cancer cells, and its mechanism may be related to targeted regulation of FBXW7 expression.

Key words

Micro RNA-27a / Cervical cancer / F-box and WD repeat domain containing protein 7 / Real-time PCR / Flow cytometry / Western blotting / Human

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ZHANG Hong-ping LI Feng NIU Zhan-jie. Expression and mechanism of microRNA-27a in cervical cancer[J]. Acta Anatomica Sinica. 2021, 52(1): 78-83 https://doi.org/10.16098/j.issn.0529-1356.2021.01.012

References

［1］ Leva GD, Garofalo M, Croce CM. MicroRNAs in cancer［J］. Annu Rev Pathol, 2014, 9: 287-314.

［2］ Wang JY, Chen LJ. The role of microRNAs in the invasion and metastasis of cervical cancer［J］. Biosci Rep, 2019, 39(3): BSR20181377.

［3］ Sun YD, Zhou J, Zhao ZhQ, et al. Advances in the study of microRNA-27a in regulating vascular system diseases ［J］.Chinese Journal of Vascular Surgery (Electronic Edition), 2018,10(2): 150-153, 160.(in Chinese)

孙羽东,周建,赵志青,等. miR-27a参与调控血管系统疾病研究进展［J］.中国血管外科杂志(电子版),2018,10(2):150-153, 160.

［4］ Bray F, Ferlay J, Soerjomataram Ⅰ, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］. CA Cancer J Clin, 2018, 68(6):394-424.

［5］ Farh KK, Grimson A, Jan C, et al. The widespread impact of mammalian MicroRNAs on mRNA repression and evolution［J］.Science, 2005, 310(5755):1817-1821．

［6］ Ji W, Sun B, Su C. Targeting microRNAs in cancer gene therapy［J］. Genes (Basel), 2017, 8(1): 21.

［7］ Li ChF, Jing W, Xiang DSh, et al. Expression of microRNA-613 in human glioma and its effect on proliferation and metastasis ［J］.Acta Anatomica Sinica, 2018,49 (5):579-583. (in Chinese)

李传芬,荆文,向冬生,等.MicroRNA-613在人胶质瘤中的表达及其对增殖与转移的影响［J］.解剖学报,2018,49(5):579-583.

［8］ Zhang Y, Yang Y, Liu R, et al. Downregulation of microRNA-425-5p suppresses cervical cancer tumorigenesis by targeting AIFM1［J］. Exp Ther Med, 2019, 17(5):4032-4038.

［9］ Wang L, Wang W, Wu Y. MicroRNA-26b acts as an antioncogene and prognostic factor in cervical cancer［J］. Oncol Lett, 2019, 17(3):3418-3424.

［10］ Kong Q, Tang Z, Xiang F, et al. Diagnostic value of serum hsa-mir-92a in patients with cervical cancer［J］. Clin Lab, 2017, 63(2): 335-340.

［11］ Zhang LY, Chen Y, Jia J, et al. MiR-27a promotes EMT in ovarian cancer through active Wnt/β-catenin signalling by targeting FOXO1［J］. Cancer Biomark, 2019, 24(1):31-42.

［12］ Gao W, Hong Z, Huang H,et al. miR-27a in serum acts as biomarker for prostate cancer detection and promotes cell proliferation by targeting Sprouty2［J］. Oncol Lett, 2018, 16(4):5291-5298.

［13］ Ba S, Xuan Y, Long ZW,et al. MicroRNA-27a promotes the proliferation and invasiveness of colon cancer cells by targeting SFRP1 through the Wnt/β-catenin signaling pathway［J］. Cell Physiol Biochem, 2017, 42(5):1920-1933.

［14］ Wu F, Li J, Guo N, et al. MiRNA-27a promotes the proliferation and invasion of human gastric cancer MGC803 cells by targeting SFRP1 via Wnt/β-catenin signaling pathway［J］. Am J Cancer Res, 2017, 7(3):405-416.

［15］ Chen S, Sun YY, Zhang ZX, et al. Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway［J］. Oncotarget, 2017, 8(9):14708-14718.

［16］ Lin T, Ma Q, Zhang Y, et al. MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1［J］. Oncol Lett, 2018, 15(1):1067-1071.

［17］ Gocze K, Gombos K, Juhasz K, et al. Unique microRNA expression profiles in cervical cancer［J］. Anticancer Res, 2013, 33(6):2561-2567.

［18］ Gocze K, Gombos K, Kovacs K, et al. MicroRNA expressions in HPV-induced cervical dysplasia and cancer［J］. Anticancer Res, 2015, 35(1):523-530.

［19］ Jiang G, Shi W, Fang H,et al. miR 27a promotes human breast cancer cell migration by inducing EMT in a FBXW7 dependent manner［J］. Mol Med Rep, 2018, 18(6):5417-5426.

［20］ Liu Z, Liu X, Liu S,et al. Cholesterol promotes the migration and invasion of renal carcinoma cells by regulating the KLF5/miR-27a/FBXW7 pathway［J］. Biochem Biophys Res Commun,2018, 502(1):69-75.

［21］ Wu XZ, Wang KP, Song HJ, et al. MiR-27a-3p promotes esophageal cancer cell proliferation via F-box and WD repeat domain-containing 7 (FBXW7) suppression［J］. Int J Clin Exp Med, 2015, 8(9):15556-15562.

［22］ Yeh CH, Bellon M, Nicot C. FBXW7: a critical tumor suppressor of human cancers［J］. Mol Cancer, 2018, 17(1):115.

［23］ Xu Y, Yu J, Liu T,et al. Loss of FBXW7 is related to the susceptibility and poor prognosis of cervical squamous carcinoma［J］. Biomarkers, 2016, 21(4):379-385.

［24］ Zhou C, Shen L, Mao L, et al. miR-92a is upregulated in cervical cancer and promotes cell proliferation and invasion by targeting FBXW7［J］. Biochem Biophys Res Commun, 2015, 458(1):63-69.