MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer&nbsp; cell migration and invasion

WANG Li-Na; CUI Na

doi:10.16098/j.issn.0529-1356.2021.01.011

PDF(4562 KB)

Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (1) : 73-77. DOI: 10.16098/j.issn.0529-1356.2021.01.011

MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer cell migration and invasion

WANG Li-na¹ LIU Yong-juan¹ ZHANG Ying¹ LIU Rong-xia¹ LIANG Shan¹ SONG Chun-hong² CUI Na^3*

Author information +

History +

Abstract

Objective To investigate the expression of microRNA (miR)-513c-5p in cervical cancer and the mechanism of targeting histone deacetylase 1 (HDAC1) regulating cervical cancer cell migration and invasion. Methods Clinically collected 86 patients with cervical cancer. The levels of miR-513c-5p in tumor tissues and adjacent tissues were detected by Real-time PCR. The relationship between miR-513c-5p and pathological characteristics of cervical cancer was analyzed. It was verified that miR-513c-5p targets HDAC1 by a dual luciferase report. Cervical cancer HeLa cells were divided into four groups: control group, mimic group, mimic+HDAC1 group and HDAC1 group. MiR-513c-5p and(or) HDAC1 were overexpressed by plasmid transfection technology. Real-time PCR and Western blotting were used to detect the expression level of RNA or protein, respectively. The cell growth, migration, and invasion capabilities of each group were measured by CCK-8 method , cell scratch test, and Transwell test. Results The level of miR-513c-5p in cervical cancer tissues was significantly lower than that in adjacent tissues. Low levels of miR-513c-5p were associated with higher local invasion, lymphatic metastasis, and distal metastasis (P<0.05). MiR-513-5p targeted HDAC1 expression. Overexpression of miR-513c-5p inhibited significantly the growth, migration and invasion of cervical cancer cells (P<0.05). Overexpression of HDAC1 promoted growth, migration and invasion (P<0.05), and reversed the inhibitory effect of miR-513c-5p (P<0.05). Conclusion Low levels of miR-513c-5p might be related to cervical cancer metastasis, and miR-513c-5p could inhibit the growth, migration and invasion of cervical cancer HeLa cells by targeted inhibition of HDAC1 protein expression.

Key words

Cervical cancer / MicroRNA-513c-5p / Histone deacetylase 1 / Migration / Invasion / Dual luciferase report / Real-time PCR / Western blotting / Human

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WANG Li-na LIU Yong-juan ZHANG Ying LIU Rong-xia LIANG Shan SONG Chun-hong CUI Na. MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer cell migration and invasion[J]. Acta Anatomica Sinica. 2021, 52(1): 73-77 https://doi.org/10.16098/j.issn.0529-1356.2021.01.011

References

［1］ Gu XY, Zheng RS, Sun KX, et al. Incidence and mortality of cervical cancer in China, 2014［J］. Zhonghua Zhong Liu Za Zhi, 2018, 40(4): 241-246.

［2］ Kim SY, Kawaguchi T, Yan L, et al. Clinical relevance of microRNA expressions in breast cancer validated using the cancer genome atlas (TCGA)［J］. Ann Surg Oncol, 2017, 24(10): 1-7.

［3］ Zhang K, Zhao Z, Yu J, et al. LncRNA FLVCR1-AS1 acts as miR-513c sponge to modulate cancer cell proliferation, migration, and invasion in hepatocellular carcinoma［J］. J Cell Biochem, 2018, 119(7): 6045-6056.

［4］ Xiao L, Wan YZ, Wang B, et al. Association between expression of HDAC1 protein and HPV16/18 infection in cervical intraepithelial neoplasia and cervical squamous cell carcinoma［J］. Journal of China Medical University, 2015, 44(5):464-467. (in Chinese)

肖莉, 万义增, 王宝, 等. 宫颈上皮内瘤变及鳞癌组织中组蛋白去乙酰化酶1的表达及与HPV16/18感染的关系［J］. 中国医科大学学报, 2015, 44(5):464-467.

［5］ Tomao F, Maruccio M, Preti EP, et al. Conization in early stage cervical cancer: pattern of recurrence in a 10-year single-institution experience［J］. Int J Gynecol Cancer, 2017, 27(5): 1001-1008.

［6］ Kong Q, Tang Z, Xiang F, et al. Diagnostic value of serum hsa-mir-92a in patients with cervical cancer［J］. Clin Lab, 2017, 63(2): 335-337.

［7］ Chen J, Jiang CC, Jin L, et al. Regulation of PD-L1: a novel role of pro-survival signaling in cancer［J］. Ann Oncol, 2015, 27(3): 615-617.

［8］ Xia HL, Lv Y, Xu CW, et al. MiR-513c suppresses neuroblastoma cell migration, invasion, and proliferation through direct targeting glutaminase (GLS)［J］. Cancer Biomark, 2017, 20(4): 589-596.

［9］ Dong P, Xiong Y, Yue J, et al. miR-34a, miR-424 and miR-513 inhibit MMSET expression to repress endometrial cancer cell invasion and sphere formation［J］. Oncotarget, 2018, 9(33): 23253-23263.

［10］ Mosakhani N, Pazzaglia, Benassi MS, et al. MicroRNA expression profiles in metastatic and non-metastatic giant cell tumor of bone［J］. Histol Histopathol, 2012, 28(5): 671-678.

［11］ Qiao W, Liu H, Liu R, et al. Prognostic and clinical significance of histone deacetylase 1 expression in breast cancer: a meta-analysis［J］. Clin Chim Acta, 2018, 483(1):209-215.

［12］ Cao LL, Song X, Pei L, et al. Histone deacetylase HDAC1 expression correlates with the progression and prognosis of lung cancer: a meta-analysis［J］. Medicine, 2017, 96(31): e7663-7666.

［13］ Gupta R, Bhatt LK, Momin M. Potent antitumor activity of Laccaic acid and phenethyl isothiocyanate combination in colorectal cancer via dual inhibition of DNA methyltransferase-1 and histone deacetylase-1 ［J］. Toxicol Appl Pharmacol, 2019, 19(377): e114631-114633.

［14］ Lee JW, Yang DH, Park S, et al. Trichostatin A resistance is facilitated by HIF-1α acetylation in HeLa human cervical cancer cells under normoxic conditions［J］. Oncotarget, 2017, 9(2): 2035-2049.

［15］ Paydar I, Velena A, Grindrod S, et al. Abstract 5855: radiosensitization of cervical cancer cells by HDAC inhibitors［J］. Cancer Res, 2017, 77(13 Suppl): 5855.