Effects of Rab1A on proliferation and apoptosis of multiple myeloma cell line 8226

WU Han

doi:10.16098/j.issn.0529-1356.2021.01.009

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (1) : 60-66. DOI: 10.16098/j.issn.0529-1356.2021.01.009

Cancer Biology

Effects of Rab1A on proliferation and apoptosis of multiple myeloma cell line 8226

WU Han* WANG Xiu-hong WU Ting-ting YANG Su

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Abstract

Objective To investigate the effect of Rab1A on proliferation and apoptosis of multiple myeloma(MM) cell line 8226. Methods The siRNA interference was used to knockdown Rab1A gene. The multiple myeloma cell line 8226 was divided into blank control group, negative control group and Rab1A siRNA group. In the blank control group, the multiple myeloma cells were not treated. Multiple myeloma cells 8226 in the negative control group were transfected with negative control siRNA. The Rab1A siRNA group was transfected with Rabl A-targeted siRNA. The effect of Rab1A on multiple myeloma cell 8226 proliferation was analyzed by colony forming test and cell counting kit-8 (CCK-8) assay. The apoptosis of multiple myeloma cell 8226 was detected by flow cytometry. Western blotting and Real-time PCR were used to observe the effect of Rab1A siRNA on the expression of c-Myc, cyclin D1, Bcl-2 and Bax. Results The expressions of Rab1A mRNA and Rab1A protein in the Rab1A siRNA group were significantly down-regulated compared with those in the negative control group. The result of colony formation and CCK-8 assay showed that Rab1A siRNA inhibit the proliferation of multiple myeloma cells 8226. The early and late apoptosis ratio of multiple myeloma cell 8226 in Rab1A siRNA group increased significantly compared with the negative control group (P<0.05). The expression of cyclin D1 and Bcl-2 in the Rab1A siRNA group were significantly down-regulated compared with the negative control group (P<0.05), and the expression of c-Myc and Bax were significantly up-regulated compared with the negative control group (P<0.05). ConclusionRab1A may promote the proliferation of multiple myeloma cells 8226 by regulating the expression of c-Myc, cyclin D1, Bcl-2 and Bax, while RablA siRNA can effectively inhibit the expression of RablA in rpmi-8226 cells, thereby inhibiting its proliferation and promoting apoptosis.

Key words

Rab1A / Multiple myeloma cell / Proliferation / Western blotting / Human

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WU Han WANG Xiu-hong WU Ting-ting YANG Su. Effects of Rab1A on proliferation and apoptosis of multiple myeloma cell line 8226[J]. Acta Anatomica Sinica. 2021, 52(1): 60-66 https://doi.org/10.16098/j.issn.0529-1356.2021.01.009

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