Effects of moderate intensity exercise on osteoporosis and bone morphogenetic protein 2 signaling pathway in ovariectomized rats

ZHANG Shuai-Jun

doi:10.16098/j.issn.0529-1356.2020.06.020

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (6) : 934-939. DOI: 10.16098/j.issn.0529-1356.2020.06.020

Histology,Embryology and Developmental Biology

Effects of moderate intensity exercise on osteoporosis and bone morphogenetic protein 2 signaling pathway in ovariectomized rats

ZHANG Shuai-jun*

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Abstract

Objective To investigate the effects of moderate intensity exercise on bone mineral density, bone metabolism, bone biomechanics and bone morphogenetic protein 2(BMP-2) signal pathway in ovariectomized rats. Methods Twenty-four adult female SD rats were randomly divided into sham operation group, operation group and exercise group with 8 rats in each group. The sham operation group only had bilateral peri-ovarian adipose tissue excised, while the operation group and the exercise group had bilateral ovaries excised. One week after ovariectomy, the exercise group received moderate intensity exercise training for 12 weeks. After 12 weeks, bone mineral density（BMD）, biomechanics, bone metabolism and histology of femur were measured, and the expression of BMP-2, Runt-related transcription factor 2(Runx2), Osterix and SMAD1 / 5 / 8 in femur were detected by Western blotting. Results The levels of serum calcium, phosphorus and tartrate resistant acid phosphatase in operation group and exercise group were higher than those in sham operation group (P<0.05), while those in exercise group were lower than those in operation group (P<0.05).BMD in operation group and exercise group was lower than that in sham operation group (P<0.05), while that in exercise group was higher than that in operation group(P<0.05).The biomechanical properties of bone tissue in operation group and exercise group were lower than that in sham operation group(P<0.05), while that in exercise group was higher than that in operation group(P<0.05).Histology showed that the degree of osteoporosis in exercise group was less than that in operation group, and the expression of BMP-2 in bone tissue was more than that in operation group. The expression of BMP-2, Runx2, Osterix and SMAD1/5/8 in operation group and exercise group was lower than that in sham operation group (P<0.05) but the expression of these proteins in exercise group was higher than that in operation group. Conclusion Moderate intensity exercise can improve bone mineral density, biomechanical properties and bone metabolism of femur in ovariectomized rats, which may be related to BMP-2 signaling pathway.

Key words

Osteoporosis / Moderate intensity exercise training / Bone mineral density / Bone metabolism / Bone biomechanics / Bone morphogenetic protein 2 signaling pathway / Western blotting / Rat

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ZHANG Shuai-jun. Effects of moderate intensity exercise on osteoporosis and bone morphogenetic protein 2 signaling pathway in ovariectomized rats[J]. Acta Anatomica Sinica. 2020, 51(6): 934-939 https://doi.org/10.16098/j.issn.0529-1356.2020.06.020

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