&nbsp;MicroRNA-423-5p reducing the injury of human umbilical vascular endothelial cells induced by lipopolysaccharide through targeting aldehyde dehydrogenase 2

LIU Wen-Fang

doi:10.16098/j.issn.0529-1356.2020.06.010

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (6) : 868-876. DOI: 10.16098/j.issn.0529-1356.2020.06.010

MicroRNA-423-5p reducing the injury of human umbilical vascular endothelial cells induced by lipopolysaccharide through targeting aldehyde dehydrogenase 2

LIU Wen-fang*

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Abstract

Objective To investigate the protective effect of microRNA-423-5p(miR-423-5p) on injury of human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide(LPS) and its potential mechanism. Methods The HUVECs were induced by 1 mg/L LPS for 24 hours, then expression levels of miR-423-5p and aldehyde dehydrogenasel 2 (ALDH2) in HUVECs induced by LPS were determined by Real-time PCR and Western blotting. The miR-423-5p was down-regulated and ALDH2 was up-regulated by transfected with anti-miR-423-5p and pcDNA-ALDH2. The apoptotic rate of HUVECs was detected by flow cytometry, and the expression levels of Bcl-2 and Bax were measured by Western blotting. The levels of interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in supernatant of HUVECs induced by LPS were determined by the kits. The relationship between miR-423-5p and ALDH2 was validated by dual-luciferase reporter assay system. Results Compared with the control group, LPS induced apoptosis and injury of HUVECs. After LPS induction, the levels of miR-423-5p, Bax, IL-6 and TNF-α in HUVECs increased significantly (P<0.05), and the levels of Bcl-2, ALDH2 mRNA and ALDH2 protein decreased remarkably (P<0.05). Either down-regulation of miR-423-5p or over-expression of ALDH2 alleviated the injury of HUVECs induced by LPS and inhibited apoptosis. MiR-423-5p targeted and negatively regulated the expression of ALDH2. Inhibition of ALDH2 reversed the effects of miR-423-5p down-regulation on the injury of HUVECs induced by LPS.

Conclusion MiR-423-5p alleviates the injury of HUVECs induced by LPS and inhibits apoptosis by targeting ALDH2.

Key words

Umbilical vein endothelial cell / Lipopolysaccharide / MicroRNA-423-5p / Aldehyde dehydrogenase 2 / Real-time PCR / Flow cytometry / Human

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LIU Wen-fang. MicroRNA-423-5p reducing the injury of human umbilical vascular endothelial cells induced by lipopolysaccharide through targeting aldehyde dehydrogenase 2[J]. Acta Anatomica Sinica. 2020, 51(6): 868-876 https://doi.org/10.16098/j.issn.0529-1356.2020.06.010

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