Effcts of adenosine preconditioning on the expression of tumor necrosis factor α&nbsp; and nuclear factor κB in rats after cerebral ischemia-reperfusion injury

YIN Xue-Yan; GUO Shao-Kai; TAN Jun; FAN Wen-Tong; LI Ming

doi:10.16098/j.issn.0529-1356.2020.06.006

PDF(10415 KB)

Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (6) : 839-847. DOI: 10.16098/j.issn.0529-1356.2020.06.006

Effcts of adenosine preconditioning on the expression of tumor necrosis factor α and nuclear factor κB in rats after cerebral ischemia-reperfusion injury

YIN Xue-yan¹ GUO Shao-kai¹ TAN Jun^2* FAN Wen-tong³ LI Ming¹

Author information +

History +

Abstract

Objective To observe the effect of adenosine preconditioning on the expression of tumor necrosis factor α（TNF-α）and nuclear factor κB (NF-κB)in rats after cerebral ischemia-reperfusion(IR) injury,and to explore the protective mechanism of adenosine on focal cerebral ischemia-reperfusion injury in rats. Methods Total sixty Sprague-Dawley rats were randomly divided into sham group (F group),ischemia-reperfusion group (IR group) and adenosine preconditioning ischemia-reperfusion group (AP group). Each group was randomly divided into 5 mice at 2 hour, 6 hour, 24 hour and 48 hour after ischemia-reperfusion. The rat moadel of middle cerebral artery occlusion (MCAO) was established by suppository method . 48 hours after ischemia-reperfusion, line head MRI, HE staining and for more than of 60 neursl function defect scale in rats，integral optical density of the software system by immunohistochemical method in the observation group rats TNF-α and NF-κB average absorbance values of rats’brain tissues to further verify the adenosine preconditioning on rat cerebral ischemia-reperfusion injury，adenosine on rat focal cerebral ischemia-reperfusion injury of protection mechanism. Results No signs of neurological defects was found in the rats in F after surgery, and obvious signs of neurological defects were found in the rats of AP and IR groups.The neurological defect scores of the AP group and the IR group were significantly higher than those of the F group, and the neurological defect scores of the AP group were significantly lower than those of the IR group, with statistically significant differences(P<0.05).Postoperative MRI images of T1WI and T2WI of rats in group F showed no obvious abnormalities. The cerebral infarction volume of rats in the AP group and the IR group was significantly higher than those of the F group, and the cerebral infarction volume of rats in the AP group was significantly lower than those of the IR group,with statistically significant differences(P<0.05).The expressions of TNF-α and NF-κB in the brain tissues of the AP group and the IR group were significantly higher than those of the F group, and the expressions of TNF-α and NF-κB in the brain tissues of the AP group was significantly lower than those of the IR group, with statistically significant differences(P<0.05). Conclusion Adenosine preconditioning can protect the brain by descreasing the expression of TNF-α and NF-κB in rats with cerebral ischemia-reperfusion injury.

Key words

Adenosine / Pretreatment / Ischemia-reperfusion / Tumor necrosis factor α / Nuclear factor κB / Immunohistochemistry / Rat

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

YIN Xue-yan GUO Shao-kai TAN Jun FAN Wen-tong LI Ming. Effcts of adenosine preconditioning on the expression of tumor necrosis factor α and nuclear factor κB in rats after cerebral ischemia-reperfusion injury[J]. Acta Anatomica Sinica. 2020, 51(6): 839-847 https://doi.org/10.16098/j.issn.0529-1356.2020.06.006

References

［1］ Zhang W, Fan YW, Gao J, et al. Review of relevant literature on epidemiological investigation of stroke ［J］. Chinese Clinical Neuroscience, 2014,22 (6):699-703.(in Chinese)

张薇,范宇威,高静,等.脑卒中流行病学调查相关文献复习［J］.中国临床神经科学,2014,22（6）：699-703.

[2］ Liu L, Liu LH, Ma YK. Research progress in the mechanism of cerebral ischemia-reperfusioninjury ［J］. Pharmaceutical Research,2016-35 (9):542-544. (in Chinese)

刘磊,刘丽华,马玉奎.脑缺血再灌注损伤机制研究进展［J］.药学研究,2016-35（9）：542-544.

［3］ Dong MZh, Deng Y, Liu F, et al. Research progress in the mechanism of cerebral ischemia-reperfusion injury ［J］. Grass-Roots Medical Forum, 2018,22 (14):1981-1982. (in Chinese)

董梦珍,邓云,刘飞,等.脑缺血再灌注损伤机制研究进展［J］.基层医学论坛,2018,22（14）：1981-1982.

［4］ Zhang BY, Ma LL, Zhu L, et al. Research status of autophagy in ischemia reperfusioninjury ［J］. World Latest Medical Information Digest,2017,17 (26):65-66. (in Chinese)

张冰缘,马丽丽,朱琳,等.自噬在缺血再灌注损伤中的研究现状［J］.世界最新医学信息文摘,2017,17（26）：65-66.

［5］ Huang TT, Teng L, Huang H, et al. Effects of butylphthalide pretreatment on neurological deficit score, oxidative damage and morphology in rats with cerebral ischemia-reperfusion injury ［J］. Acta Anatomica Sinica, 2014, 45 (5):622-626. (in Chinese)

黄婷婷,滕蕾,黄惠,等，丁苯酞预处理对脑缺血再灌注损伤大鼠神经功能缺损评分、氧化损伤和形态学的影响［J］.解剖学报，2014,45（5）：622-626.

[6］ Eitzschig HK, Sitkovsky MV, Robson SC. Purinergic signaling during inflammation ［J］.N Eng J Med, 2013,368(13):1260.

［7］ Ohta A, Sitkovsky M. Role of protein-coupled adenosine receptors in downregulation of inflammation and peotection from tissue damage ［J］.Nature,2001,414(6866):916-920.

［8］ Yu N, Tan J, Lu T. Protective effect and mechanism of adenosine preconditioning on ischemia reperfusion injury in rats ［J］. Chinese Journal of Applied Neurological Diseases,2011,14 (3): 1-3. (in Chinese)

尉娜,谭军,路坦.腺苷预处理对大鼠缺血再灌注损伤的保护作用和机制［J］.中国实用神经疾病杂志,2011,14（3）：1-3.

［9］ Tao X, Kuang PG, Ming J, et al. Protective effects of adenosine on lipid peroxidation during ischemia-reperfusion injury ［J］. Journal of Brain and Nervous Diseases, 1996, 4 (1):1-3. (in Chinese)

陶昕,匡培根,名晶，等.腺苷对缺血再灌注损伤时的保护作用——抗脂质过氧化［J］.脑与神经疾病杂志,1996，4（1）：1-3.

［10］ Tan J, Sun ZhY. Effects of adenosine preconditioning on glial neurotrophic factor expression after focal cerebral ischemia-reperfusion injury in rats ［J］. Chinese Journal of Practical Medicine, 2008,35 (21): 1-3. (in Chinese)

谭军,孙兆印.腺苷预处理对大鼠局灶性脑缺血再灌注损伤后胶质源性神经营养因子表达的影响［J］.中国实用医刊，2008，35（21）:1-3.

［11］ Zhang Y, Chen J, Han YY, et al. Effects of adenosine preconditioning on hypoxia-inducer-1 expression in brain tissue of rats with focal cerebral ischemia-reperfusion injury ［J］. Journal of Xinxiang Medical College, 2014, 31(4): 272-274. (in Chinese)

张妤,陈娟,韩艳艳,等.腺苷预处理对局灶性脑缺血再灌注损伤大鼠脑组织中低氧诱导因子-1α表达的影响［J］.新乡医学院校报，2014,31(4)：272-274．

［12］ Yu N, Wang JP, Shen XL, et al. Adenosine preconditioning on VEGF expression after cerebral ischemia-reperfusion injury in rats ［J］. Journal of Stroke and Neurological Disease, 2014, 31 (2):107-109. (in Chinese)

尉娜,王建平,申小龙,等.腺苷预处理对大鼠脑缺血再灌注损伤后VEGF的表达［J］.中风与神经疾病杂志，2014,31（2）：107-109.

［13］ Wang J, Han YY, Li J, et al. Changes of CX3CL1 and glt-1 expression in astrocytes after adenosine pretreatment with oxyglucose deprivation reoxygenation ［J］. Chinese Journal of Geratology,2017, 37(2):288-291. (in Chinese)

王洁,韩艳艳,李娟,等.腺苷预处理星形胶质细胞氧糖剥夺复氧糖后CX3CL1、GLT-1表达的变化［J］.中国老年学杂志,2017,37(2):288-291.

［14］ Niu CY, Hu YN,Wang J, et al. Effects of adenosine preconditioning on expression of astrocytes and lipid carrier protein-2 induced by glucose deprivation/reoxygenation ［J］. Chinese Journal of Anatomy, 2016,39(2):192-195. (in Chinese)

牛草原,胡亚男,王洁,等.腺苷预处理对氧糖剥夺/复氧糖诱导的星形胶质细胞及脂质运载蛋白2表达的影响［J］.解剖学杂志,2016,39(2):192-195.

［15］ Zhao X, Pei KY, Tan J. Adenosine preconditioning on focal cerebral ischemia reperfusion in rats after mitochondrial reactive oxygen content and mitochondrial respiratory chain complexes Ⅰ, Ⅲ activity of brain tissue and total antioxidant capacity influence ［J］. Journal of Xinxiang Medical College, 2019, 36(4): 305-308. (in Chinese)

赵欣,裴科阳,谭军.腺苷预处理对大鼠局灶性脑缺血再灌注后线粒体活性氧含量及线粒体呼吸链复合体Ⅰ、Ⅲ活性和脑组织总抗氧化能力的影响［J］.新乡医学院学报,2019,36(4):305-308.

［16］ Pei KY, Tan J, Xia YH, et al. Adenosine preconditioning on mitochondrial function of nerve cells in rats with focal cerebral ischemia-reperfusion injury ［J］. Shandong Medical Journal, 2008,58(27):43-46. (in Chinese)

裴科阳,谭军,夏艳红,等.腺苷预处理的局灶性脑缺血再灌注损伤大鼠神经细胞线粒体功能观察［J］.山东医药,2018,58(27):43-46.

［17］ Yu N, Zhao JH, Li J, et al. Effects of adenosine preconditioning on Wnt1 expression in rats with focal cerebral ischemia-reperfusion injury ［J］. Journal of Stroke and Neurological Disease, 2015,32(8):691-694. (in Chinese)

尉娜,赵建华,李娟,等.腺苷预处理对局灶性脑缺血再灌注损伤大鼠脑内Wnt1表达的影响［J］.中风与神经疾病杂志,2015,32(8):691-694.

［18］ Read MA, Whitley MZ ,Williams AJ, et al.NF-Kappa B and I kappa Bα：an inducible regulatory system in endothelial activation［J］.J Exp Med,1994,179（2）:503-512.

［19］ Feng ChSh, Wang HSh, Li S. Expression of nuclear transcription factor B after transient focal cerebral ischemia/reperfusion in rats ［J］. Journal of Stroke and Neurological Diseases, 2003,20(1):11-13. (in Chinese)

冯春生,王虎山,李淞.大鼠短暂局灶性脑缺血再灌注后核转录因子Κb的表达［J］.中风与神经疾病杂志,2003,20(1):11-13.

［20］ Zhang DM,Liu JX, Chen HB, et al. Expression of NF- B and il-6 in the brain of rats with focal cerebral ischemia-reperfusion injury ［J］. Chinese Journal of Geratology, 2005, 25 (11): 1364-1366. (in Chinese)

张冬梅,刘举祥,陈红兵,等.NF-κB和IL-6在局灶性脑缺血再灌注损伤大鼠脑组织中的表达［J］.中国老年学杂志,2005,25(11):1364-1366.

［21］ Mecocci P, Mariani E, Polidori MC, et al. Antioxidant agents in Alzheimer’s disease［J］.Cent Nerv Syst Agents Med Chem,2008,8(1):48-63.

［22］ Li YSh, Ding SJ, Ni CR. Effects of nuclear transcription factor -B on apoptosis of rat nerve cells after cerebral ischemia-reperfusion and its mechanism ［J］. Practical Journal of Medicine & Pharmacy, 2005,22(8):706-709. (in Chinese)

李永生,丁素菊,倪灿荣.核转录因子-κB对鼠脑缺血再灌注后神经细胞凋亡的影响及机制［J］.实用医药杂志,2005,22(8):706-709.

［23］ Taylor CT,Dzus Aland ,Colgan SP. Autocrine regulation of epithelial permeability by hypoxia：role for polarized release of tumor necrosis factor alpha［J］. Gastroenterology, 1998, 114(4):657-668.

［24］ Trickler WJ, Mayhan WG, Miller DW. Brain microvessel endothelial cell responses to tumor necrosis factor-alpha involve a nuclear factor kappaB （NF-kappaB）signal transduction pathway［J］.Brain Res, 2005,1048(1-2):24-31.

［25］ Li JJ, Jiang HY, Shao JL. Expression of tnf-a, il-6, il-1 in brain tissue of rats with cerebral ischemia-reperfusion injury ［J］. Journal of Kunming Medical University, 2016,37 (9):31-35. (in Chinese)

李俊杰,蒋海燕,邵建林.脑缺血-再灌注损伤大鼠脑组织中TNF-a,IL-6,IL-1β的表达［J］.昆明医科大学学报,2016,37（9）:31-35.