Effect of Astragalus, Epimedium and Pueraria compound on Alzheimer’s disease mice behaviors and hippocampus disintegrin and metalloprotease 10 expression#br#

MA Dong-Xue; GAO Wei-Juan; HE Xiao-Ping; XU Li-Jun; LIU Ya-Lei; DONG Xian-Hui

doi:10.16098/j.issn.0529-1356.2020.06.003

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (6) : 821-826. DOI: 10.16098/j.issn.0529-1356.2020.06.003

Effect of Astragalus, Epimedium and Pueraria compound on Alzheimer’s disease mice behaviors and hippocampus disintegrin and metalloprotease 10 expression#br#

MA Dong-xue1^,2 GAO Wei-juan²HE Xiao-ping²XU Li-jun² LIU Ya-lei² DONG Xian-hui^2*

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Abstract

Objective To investigate the effects of a combination of Astragalus, Epimedium and Pueraria effective extracts on behaviors and the expression of a disintegrin and metalloprotease 10 (ADAM10) in hippocampal CA3 region of APPswe/PS1dE9 double transgenic Alzheimer’s disease (AD) model mice. Methods Thirty 10-month-old male APPswe/PS1dE9 double transgenic model mice were randomly divided into compound group, model group and the deferox (DFX) group, and 10 age-matched male C57BL/6 J mice were used as normal controls. After the medication was over, the learning and memory ability of mice in each group was measured by Morris water maze, and the brain tissue was taken out after the water maze test. The expression of ADAM10 in hippocampal CA3 region of mice in each group was detected by immunofluorescence, Real-time PCR and Western blotting. Results Compared with the normal control group, the escape latency, the swimming distance and the swimming time of the model group mice were significantly prolonged (P<0.05),crossing-target number and target-quadrant abidance were significantly decreases (P<0.05)，escape latency for first crossing the platform was significantly prolonged(P<0.05)；The escape latency, swimming distance and swimming time of astragalus, epimedium and pueraria effective component compound group and DFX group were significantly shorter than those of APPswe/PS1dE9 double transgenic AD model mice (P<0.05). Crossing-target number and target-quadrant abidance were significantly prolonged (P<0.05)，escape latency for first crossing the platform was significantly decreases (P<0.05)；There was no significant differences in escape latency, swimming distance, swimming time and cross times between the compound group and the DFX group (P>0.05). Crossing-target number，target-quadrant abidance and escape latency for first crossing the platform wer e no significant difference. Compared with the normal control group mice, AD model group increased the expression of hippocampal CA3 area ADAM10 (P<0.05), and the expression of ADAM10 in the hippocampal CA3 region of the compound group and DFX group was lower than that of the model group (P<0.05), but there was no significant difference in the expression of ADAM10 between the compound group and the DFX group. Conclusion The effective components of Herba Epimedii, Astragalus membranaceus and Pueraria lobata could improve the learning and memory ability of APPswe/PS1dE9 double transgenic AD model mice, and the mechanism might be related to the down-regulation of ADAM10 expression and the reduction of neuron damage and death.

Key words

Alzheimer’s disease / Epimedium / Astragalus / Radix puerariae / A disintegrin and metalloprotease 10 / Western blotting / APPswe/PS1dE9 double transgenic model mouse

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MA Dong-xue GAO Wei-juan HE Xiao-ping XU Li-jun LIU Ya-lei DONG Xian-hui. Effect of Astragalus, Epimedium and Pueraria compound on Alzheimer’s disease mice behaviors and hippocampus disintegrin and metalloprotease 10 expression#br#[J]. Acta Anatomica Sinica. 2020, 51(6): 821-826 https://doi.org/10.16098/j.issn.0529-1356.2020.06.003

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