Expression of RUNX1T1 and neuronal differentiation in radial glial cells after deafferented injury of hippocampus in vitro

ZOU Lin-Qing; YI Xin; HE Hui; DAN Bai-Quan; JIN Guo-Hua; LI Hao-Ming

doi:10.16098/j.issn.0529-1356.2020.06.001

PDF(8068 KB)

Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (6) : 809-814. DOI: 10.16098/j.issn.0529-1356.2020.06.001

Expression of RUNX1T1 and neuronal differentiation in radial glial cells after deafferented injury of hippocampus in vitro

ZOU Lin-qing YI Xin HE Hui SHAN Bo-quan JIN Guo-hua LI Hao-ming*

Author information +

History +

Abstract

Objective To investigate the expression of runt-related transcription factor 1 translocated to 1 (RUNX1T1) and neuronal differentiation in hippocampal radial glial cells after deafferented injury of hippocampus in vitro. Methods After physical transecting of fimbriafornix in 6 rats to establish a model of deafferented hippocampus, the extract of hippocampus was prepared. Hippocampal radial glial cells were cultured into 6 pieces of 24 holes cell culture plates in vitro and divided into deafferented injury, normal and blank groups, 48 holes in each group. The expression of RUNX1T1 and proportion of microtubule associated protein 2 (MAP-2) positive neurons in each group were detected by immunofluorescence, Real-time PCR and Western blotting. Results Normally hippocampal radial glial cells had thin and long processes and expressed RUNX1T1. The fluorescence intensity of RUNX1T1 in deafferented injury group was about 1.8 times higher than that in normal and blank group, and the cell process was also longer. The mRNA and protein expression of RUNX1T1 in the deafferented injury group were increased by 2.9 and 2.4 times respectively, and 39.33% cells expressed MAP-2. Compared with normal and blank group, the proportion of MAP-2 positive neurons increased more. Conclusion The expression of RUNX1T1 is up-regulated in hippocampal radial glial cells after deafferented injury, and they could differentiate into more neurons in vitro.

Key words

Hippocampus / Neurogenesis / Neuron / Radial glial cell / Runt-related transcription factor 1 translocated to 1 / Western blotting / Rat

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZOU Lin-qing YI Xin HE Hui SHAN Bo-quan JIN Guo-hua LI Hao-ming. Expression of RUNX1T1 and neuronal differentiation in radial glial cells after deafferented injury of hippocampus in vitro[J]. Acta Anatomica Sinica. 2020, 51(6): 809-814 https://doi.org/10.16098/j.issn.0529-1356.2020.06.001

References

［1］ Wang JW, Stifani S. Roles of runx genes in nervous system development ［J］. Adv Exp Med Biol, 2017，962:103-116.

［2］ Linqing Z, Guohua J, Haoming L, et al. Runx1t1 regulates the neuronal differentiation of radial glial cells from the rat hippocampus ［J］. Stem Cells Transl Med, 2015，4(1):110-116.

［3］ Zhang X, Jin G, Tian M, et al. The denervated hippocampus provides proper microenvironment for the survival and differentiation of neural progenitors ［J］. Neurosci Lett, 2007,414(2):115-120．

［4］ Zou L, Jin G, Zhang X, et al. Proliferation, migration, and neuronal differentiation of the endogenous neural progenitors in hippocampus after fimbria fornix transection ［J］. Int J Neurosci, 2010,120(3):192-200.

［5］ Zou LQ, Jin GH, Wang HQ, et al. Changes of RUNX1T1 mRNA and protein expression in rat hippocampus after fimbria/fornix transection ［J］. Acta Anatomica Sinica, 2013,44(6):768-772. (in Chinese)

邹琳清, 金国华, 王海琴, 等. 穹窿海马伞切割后大鼠海马内RUNX1T1 mRNA和蛋白的表达变化［J］. 解剖学报, 2013,44(6):76-772.

［6］ Than-Trong E, Bally-Cuif L. Radial glia and neural progenitors in the adult zebrafish central nervous system ［J］. Glia, 2015,63(8):1406-1428.

［7］ Allen NJ, Lyons DA. Glia as architects of central nervous system formation and function ［J］. Science, 2018,362(6411):181-185.

［8］ Ghosh HS. Adult neurogenesis and the promise of adult neural stem cells ［J］. J Exp Neurosci, 2019, 13: 1179069519856876.

［9］ Pollen AA, Nowakowski TJ, Chen J, et al. Molecular identity of human outer radial glia during cortical development ［J］. Cell, 2015,163(1):55-67.

［10］ Wang CQ, Mok MM, Yokomizo T, et al. Runx family genes in tissue stem cell dynamics ［J］. Adv Exp Med Biol, 2017,962:117-138.

［11］ Aaker JD, Patineau AL, Yang HJ, et al. Interaction of MTG family proteins with NEUROG2 and ASCL1 in the developing nervous system ［J］. Neurosci Lett, 2010,474(1):46-51.

［12］ Zhang L, Tümer Z, Mllgrd K, et al. Characterization of a t(5;8)(q31;q21) translocation in a patient with mental retardation and congenital heart disease: implications for involvement of RUNX1T1 in human brain and heart development ［J］. Eur J Hum Genet, 2009, 17(8):1010-1018.

［13］ Sood R, Kamikubo Y, Liu P. Role of RUNX1 in hematological malignancies ［J］. Blood, 2017,129(15):2070-2082.

［14］ Alfayez M, Vishnubalaji R, Alajez NM. Runt-related transcription factor 1 (RUNX1T1) suppresses colorectal cancer cells through regulation of cell proliferation and chemotherapeutic drug resistance ［J］. Anticancer Res, 2016,36(10):5257-5263.

［15］ Sun T, Yang P, Gao Y. Long non-coding RNA EPB41L4A-AS2 suppresses progression of ovarian cancer by sequestering microRNA-103a to upregulate transcription factor RUNX1T1 ［J］. Exp Physiol, 2020,105(1):75-87.

［16］ Alishahi A, KoyanoNakagawa N, Nakagawa Y. Regional expression of MTG genes in the developing mouse central nervous system ［J］. Dev Dyn, 2009,238(8):2095-2102.