Effects of all-trans retinoic acid on parenchymal organ injury in developmental rats

WANG Xia; BAI Hao-Di; SHEN Qin; LIU Xing

doi:10.16098/j.issn.0529-1356.2020.04.018

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (4) : 576-582. DOI: 10.16098/j.issn.0529-1356.2020.04.018

Histology,Embryology and Developmental Biology

Effects of all-trans retinoic acid on parenchymal organ injury in developmental rats

WANG Xia^1,2 BAI Hao-di^1,2SHEN Qin^1,2 LIU Xing^1*

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Abstract

Objective To investigate the effects of overdose all-trans retinoic acid (ATRA) on brain, heart, lung, liver, kidney and spleen in developmental SD rats. Methods Forty-eight male SD rats, 3-week-old, were divided into control group and three treatment groups at the doses of 40, 60, and 80 mg/(kg·d) of ATRA, respectively, 12 rats each group, the rats were administrated by gavage every day for 10 days. The rats body weight were weighed every day. After treatment, the rats were sacrificed and then rat heart, lung, liver, kidney, and spleen tissues were weighed, respectively. The organ index was calculated. The organs were then stained with HE staining. Results After ATRA gavage, compared with the control group, the 40 mg/(kg·d) ATRA group had higher kidney index and body weight change was not statistically significant, the 60 mg/(kg·d) ATRA group had lower body weight, higher heart and kidney index, and lower spleen weight, and the 80 mg/(kg·d) ATRA group had significantly lower body weight, higher brain, heart and kidney index, and lower brain and spleen weight. HE staining showed that compared with the control group, SD rats in the 40, 60 and 80 mg/(kg·d) ATRA groups had thickened alveolar wall, vacuol-like changes in renal tubular epithelial cells, and more macrophages in the spleen, but no significant histological changes in the brain, liver and heart. Conclusion Excessive all-trans retinoic acid in vivo could damage the lungs, kidneys and spleen in developmental SD rats.

Key words

All-trans retinoic acid / Growth / Development / Organ damage / HE staining / Rat

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WANG Xia BAI Hao-di SHEN Qin LIU Xing. Effects of all-trans retinoic acid on parenchymal organ injury in developmental rats[J]. Acta Anatomica Sinica. 2020, 51(4): 576-582 https://doi.org/10.16098/j.issn.0529-1356.2020.04.018

References

［1］ Veldhoen M, Ferreira C. Influence of nutrient-derived metabolites on lymphocyte immunity［J］. Nat Med, 2015, 21(7): 709-718.

［2］ Homma Y, Otani N, Ishimatsu S. A case report of acute vitamin A intoxication due to ocean perch liver ingestion［J］. J Emerg Med, 2015, 49(1):15-17.

［3］ Garc ía-Cortés M, Robles-Díaz M, Ortega-Alonso A, et al. Hepatotoxicity by dietary supplements: a tabular listing and clinical characteristics［J］. Int J Mol Sci, 2016, 17(4):537-560.

［4］ Marosz A, Chlubek D. The risk of abuse of vitamin supplements［J］. Ann Acad Med Stetin Supl, 2014, 60(1):60-64.

［5］ Lionikaite Ⅴ, Westerlund A, Conaway HH, et al. Effects of retinoids on physiologic and inflammatory osteoclastogenesis in vitro［J］. J Leukoc Biol, 2018, 104(6):1133-1145.

［6］ Chen XP, Qin YH. Research advances in the protective effect of all-trans retinoic acid against podocyte injury［J］. Zhongguo Dang Dai Er Ke Za Zhi, 2017, 19(6):719-723.

［7］ Xia L, Li R, Wang Y, et al. Efficacy, safety, and cost-effectiveness of all-trans retinoic acid/Clobetasol Propionate Compound Ointment in the treatment of mild to moderate psoriasis vulgaris: a randomized, single-blind, multicenter clinical trial［J］. Dermatol Ther, 2018, 31(5):e12632.

［8］ Gold LS, Baldwin HE, Lin T. Management of severe acne vulgaris with topical therapy［J］. J Drugs Dermatol, 2017, 16(11):1134-1138.

［9］ Bouriez D, Giraud J, Gronnier C, et al. Efficiency of all-trans retinoic acid on gastric cancer: a narrative literature review［J］. Int J Mol Sci, 2018, 19(11): 3388.

［10］ Nguyen PH, Giraud J, Staedel C, et al. All-trans retinoic acid targets gastric cancer stem cells and inhibits patient-derived gastric carcinoma tumor growth［J］. Oncogene, 2016, 35(43): 5619-5628.

［11］ Wei S, Kozono S, Kats L, et al. Active Pin1 is a key target of all-trans retinoic acid in acute promyelocytic leukemia and breast cancer［J］. Nat Med, 2015, 21(5):457-466.

［12］ Schweich LC, Oliveira EJT, Pesarini JR, et al. All-trans retinoic acid induces mitochondria-mediated apoptosis of human adipose-derived stem cells and affects the balance of the adipogenic differentiation［J］. Biomed Pharmacother, 2017, 96: 1267-1274.

［13］ Obrochta KM, Krois CR, Campos B, et al. Insulin regulates retinol dehydrogenase expression and all-trans retinoic acid biosynthesis through FoxO1［J］. J Biol Chem, 2015, 290(11):7259-7268.

［14］ Qian N, Wu ShG, Guo KN, et al. Measuring of major organs and the correlation in Guizhou miniature pigs［J］. Laboratory Animal Science, 2007，24(4):27-29. (in Chinese)

钱宁，吴曙光，郭科男, 等.幼龄贵州小型猪主要脏器重量及脏器系数的测定［J］.实验动物科学，2007，24(4):27-29.

［15］ Duquette SC, Fischer CD, Feener TD, et al. Anti-inflammatory effects of retinoids and carotenoid derivatives on caspase-3-dependent apoptosis and efferocytosis of bovine neutrophils［J］. Am J Vet Res, 2014, 75(12):1064-1075.

［16］ Luo XT, Li J, Yang YJ, et al. Research progress in phosphine poisoning of forensic toxicology［J］. Modern Preventive Medicine, 2018, 45(18):35-38. (in Chinese)

罗谢添，李健，杨玉洁, 等.磷化氢中毒法医毒理学研究进展［J］.现代预防医学，2018，45(18):35-38.

［17］ He LH, Chen Ch, Li ChY, et al. Effects of heroin on the renal tissue structures and functions of mice［J］. Acta Anatomica Sinica, 2018,49(6):759-764. (in Chinese)

何玲慧, 陈诚, 李重阳,等. 海洛因对小鼠肾结构与功能的影响［J］.解剖学报,2018,49(6):759-764.

［18］ Molina-Jijón E, Rodríguez-Mu?oz R, Namorado Mdel C, et al. All-trans retinoic acid prevents oxidative stress-induced loss of renal tight junction proteins in type-1 diabetic model［J］. J Nutr Biochem, 2015, 26(5):441-454.

［19］ Dai Y, Chen A, Liu R, et al. Retinoic acid improves nephrotoxic serum-induced glomerulonephritis through activation of podocyte retinoic acid receptor α［J］. Kidney Int, 2017, 92(6):1444-1457.

［20］ Chen YCh, Chen GJ, Chen H. Advances in the relationship between M1/M2 macrophages and cardiovascular disease［J］. Current Immunology, 2015, 35(6): 507-510. (in Chinese)

陈浥尘，陈广洁，陈红. M1/M2型巨噬细胞与心血管疾病关系的研究进展［J］.现代免疫学，2015，35(6)：507-510.