Expression of miR-145-5p in colorectal cancer and its relationship with multidrug resistance

YUAN Ya-Nan; DU Meng-Nan; YAN Xiao-Li; HUANG Yan-Jie; ZHENG Ji-Ning

doi:10.16098/j.issn.0529-1356.2020.04.013

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (4) : 553-556. DOI: 10.16098/j.issn.0529-1356.2020.04.013

Cancer Biology

Expression of miR-145-5p in colorectal cancer and its relationship with multidrug resistance

YUAN Ya-nan DU Meng-nan YAN Xiao-li HUANG Yan-jie ZHENG Ji-ning*#br#

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Abstract

Objective To investigate the expression and relationship between miR-145-5p and multidrug resistance gene (MDR1) protein P-glycoprotein(P-gp) in colorectal cancer. Methods SP, Real-time PCR and Western blotting were used to detect the expression of P-gp colorectal carcinoma (CRC) in 50 patients and in 30 paracancerous normal tissue patients, the effect of miR-145-5p expression on MDR1 mRNA and P-gp and the correlation between them and clinicopathological features. Results The expression level of miR-145-5p in CRC was significantly lower than that in paracancerous normal tissue, and the expression level of MDR1 mRNA and P-gp was significantly higher than that in paracancerous normal tissue (r=-0.403, P<0.01).MiR-145-5p could inhibit the expression of MDR1 mRNA and P-gp in HCT-15 cells(P<0.05). Conclusion MiR-145-5p plays a regulatory role in the expression of multidrug resistance genes and proteins in colorectal cancer, and is involved in the occurrence, development and multidrug resistance of colorectal cancer.

Key words

Colorectal carcinoma / MiR-145-5p / P-glycoprotein / Multidrug resistance / Immunohistochemistry / Real-time PCR / Western blotting / Human

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YUAN Ya-nan DU Meng-nan YAN Xiao-li HUANG Yan-jie ZHENG Ji-ning. Expression of miR-145-5p in colorectal cancer and its relationship with multidrug resistance[J]. Acta Anatomica Sinica. 2020, 51(4): 553-556 https://doi.org/10.16098/j.issn.0529-1356.2020.04.013

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