Effect of spinal cord decellularized scaffold on the repair of spinal cord defects in rats#br#

ZHU Jun-Yi; ZHANG Yi-Jia; DU Sheng-Hu; YU Fang-Zheng; LI Pei-Feng; LU Ying-Feng; WANG Zhi-Bin; MEI Jin; WANG Jian

doi:10.16098/j.issn.0529-1356.2020.04.005

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (4) : 502-506. DOI: 10.16098/j.issn.0529-1356.2020.04.005

Neurobiology

Effect of spinal cord decellularized scaffold on the repair of spinal cord defects in rats#br#

ZHU Jun-yi¹ ZHANG Yi-jia² DU Sheng-hu¹ YU Fang-zheng¹ LU Ying-feng¹ LI Pei-feng¹ WANG Zhi-bin³ MEI Jin³ WANG Jian^1*#br#
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Abstract

Objective Exploring the effect of spinal cord decellularized scaffold on spinal cord defects and observing the behavior and regeneration of rats after operation. Methods The spinal cords of 30 SD rats were treated with 3% Triton X-100 and 2% sodium deoxycholate on oscillator. The cell residue and the spatial structure of the tissue were compared before and after treatment, in order to understand the tissue structure of the stent itself. 90 SD rats were randomly divided into control group, simple injury group and stent transplantation group. Excision of the spinal cord 9-10 segments in the simple injury group and the stent graft group the acellular scaffold was transplanted to the stent graft group. Behavioral scores were observed postoperatively. At 4, 8, and 12 weeks, the spinal cords of the injured part of the rats were taken for HE staining and immunofluorescence detection of nerve regenerationrelated proteins. Results After decellularization of the spinal cord, the nerve cells and axons were completely removed, and the extracellular matrix of the spinal cord was preserved. Scanning electron microscopy revealed that the scaffold retained a certain porous network scaffold structure. In the experiment of decellularized scaffold in vivo, the Basso-Beattie-Bresnahan(BBB) score showed that the recovery of hindlimb motor function in rats with decellularized scaffolds was better than that in rats with simple injury. HE staining showed that the decellularized scaffold could fill the defect of the spinal cord segment and accelerate the repair process of the injured spinal cord. Immunofluorescence showed that there was a certain axonal regeneration in the injured part of the stent transplantation group. Conclusion The spinal cord decellularized scaffold retains the extracellular matrix and has a certain spatial structure, which can accelerate the process of spinal cord defect repair to a certain extent, and has a certain promoting effect on nerve regeneration.

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ZHU Jun-yi ZHANG Yi-jia DU Sheng-hu YU Fang-zheng LU Ying-feng LI Pei-feng WANG Zhi-bin MEI Jin WANG Jian. Effect of spinal cord decellularized scaffold on the repair of spinal cord defects in rats#br#[J]. Acta Anatomica Sinica. 2020, 51(4): 502-506 https://doi.org/10.16098/j.issn.0529-1356.2020.04.005

References

［1］ Wang Q, Zhang C, Zhang L, et al. The preparation and comparison of decellularized nerve scaffold of tissue engineering ［J］. J Biomed Mater Res A, 2014, 102(12): 4301-4308.

［2］ Guo SZ, Ren XJ, Wu B, et al. Preparation of the acellular scaffold of the spinal cord and the study of biocompatibility ［J］. Spinal Cord, 2010,48(7):576-581.

［3］ Hosseini SM, Sharafkhah A, Koohihosseinabadi O, et al. Transplantation of neural stem cells cultured in alginate scaffold for spinal cord injury in rats［J］. Asian Spine J, 2016, 10(4):611-618.

［4］ Xue H, Zhang XY, Liu JM, et al. Development of a chemically extracted acellular muscle scaffold seeded with amniotic epithelial cells to promote spinal cord repair［J］. J Biomed Mater Res A, 2013, 101A(1); 145-156

［5］ Xu HL, Tian FR, Lu CT, et al. Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury ［J］. Sci Rep, 2016, 6:38332.

［6］ Changyu L, Xiangtong Z, Ronglong C, et al. Allografts of the acellular sciatic nerve and brain-derived neurotrophic factor repair spinal cord injury in adult rats［J］. PLoS One, 2012, 7(8):e42813.

［7］ Chen PB, Xu XF, Zhou HG. Release of SHH by acellular spinal cord scaffold promotes function recovery after spinal cord injury in rats［J］.Chinese Journal of Neuroanatomy,2018, 34(2): 203-209. (in Chinese)

陈平波, 徐晓峰, 周焕高.SHH缓释的脊髓去细胞支架促进大鼠脊髓损伤修复［J］. 神经解剖学杂志, 2018, 34(2): 203-209.

［8］ Chen J, Zhang Z, Liu J, et al. Acellular spinal cord scaffold seeded with bone marrow stromal cells protects tissue and promotes functional recovery in spinal cord-injured rats ［J］. J Neurosci Res, 2014,92(3): 307-317.

［9］ Zhang XY, Xue H, Liu JM, et al. Chemically extracted acellular muscle: a new potential scaffold for spinal cord injury repair ［J］. J Biomed Mater Res A, 2012, 100(3): 578-587.

［10］ Zhang JS，Wang H，Shao PG. Preparation and identification of a whole kidney decellularized bio-derived scaffold［J］. Acta Anatomica Sinica，2012，43(2): 253-257. (in Chinese)

张建色, 王辉, 邵培刚. 去细胞全肾生物支架的制备与鉴定［J］. 解剖学报, 2012, 43(2):253-257.

［11］ Zhao LN，Yu YL，Lin KZh. Programmed analysis of the method for the decellularization of intact rat kidney in vivo ［J］. Acta Anatomica Sinica，2014，45(2): 267-272. (in Chinese)

赵丽娜，余雅玲，林刻智. 在体制备大鼠全肾去细胞支架的程序性分析［J］. 解剖学报，2014，45(2): 267-272.

［12］ Peng MM, Lin XF, Shao PG. Preparation of heart decellularized matrix in rats by perfusing method.［J］ Acta Anatomica Sinica, 2012, 43(4):559-563. (in Chinese)

彭蒙蒙, 林贤丰, 邵培刚. 灌注法制备离体大鼠心脏脱细胞基质［J］. 解剖学报, 2012, 43(4):559-563.

［13］ Lin XF, Shao YK, Wang H. Preparation and identification of the pancreatic decellularized bio-derived scaffold in isolated rats［J］. Acta Anatomica Sinica, 2012, 43(5):717-722. (in Chinese)

林贤丰, 邵营宽, 王辉. 离体大鼠胰腺去细胞生物支架的制备与鉴定［J］. 解剖学报, 2012, 43(5): 717-722

［14］ Wang H, Lin XF, Wang LR, et al. Decellularization technology in CNS tissue repair ［J］. Expert Rev Neurother, 2015,15(5): 493-500.

［15］ Li YP， Chen XY，Zhu X. Acellular spinal cord scaffold: preparation and biological characteristics［J］. Chinese Journal of Tissue Engineering Research, 2015, 19(21): 3398-3402. (in Chinese)

李一鹏, 陈旭义, 朱祥. 脱细胞脊髓基质支架的制备及生物特性［J］. 中国组织工程研究, 2015, 19(21):3398-3402.

［16］ Yin WH, Jin DD, Deng XY. Effects of mechanical vibration on the morphology of the acellular scaffold for the spinal cord［J］.Journal of First military Medical University, 2008, 28(10): 17481751.(in Chinese)

尹文化, 金大地, 邓许勇. 机械振荡对脊髓去细胞支架形态学的影响［J］. 南方医科大学学报, 2008, 28(10): 1748-1751

［17］ Yin H, Jiang T, Deng X, et al. Acellular spinal cord scaffold seeded with rat adipose-derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats［J］. Mol Med Rep, 2018,17(2):2998-3004

［18］ Liu J, Chen J, Liu B, et al. Acellular spinal cord scaffold seeded with mesenchymal stem cells promotes long-distance axon regeneration and functional recovery in spinal cord injured rats［J］. J Neurol Sci, 2013, 325(12):127-136.

［19］ Crapo PM, Medberry CJ, Reing JE, et al. Biologic scaffolds composed of central nervous system extracellular matrix ［J］. Biomaterials, 2012, 33(13): 3539-3547.

［20］ Kim M, Park SR, Choi BH. Biomaterial scaffolds used for the regeneration of spinal cord injury (SCI)［J］. Histol Histopathol, 2014, 29(11):1395-408.

［21］ Zamani F, AmaniTehran M, Latifi M, et al. Promotion of spinal cord axon regeneration by 3D nanofibrous core-sheath scaffolds［J］. J Biomed Mater Res A, 2014, 102(2):506-513.

［22］ Shahriari D, Koffler J, Lynam DA, et al. Characterizing the degradation of alginate hydrogel for use in multilumen scaffolds for spinal cord repair［J］. J Biomed Mater Res A, 2016, 104(3):611-619.