Sudan black B reducing the intestinal autofluorescence of rats and its application in immunofluorescence

LI Yong; ZHOU Li; ZHANG Yang; LIU Zhen; YAO Nian; SHEN Feng-Ge; WANG Zhi-Yong

doi:10.16098/j.issn.0529-1356.2020.03.024

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (3) : 450-455. DOI: 10.16098/j.issn.0529-1356.2020.03.024

Technology and Methodology

Sudan black B reducing the intestinal autofluorescence of rats and its application in immunofluorescence

LI Yong¹ ZHOU Li¹ ZHANG Yang¹ LIU Zhen² YAO Nian² SHEN Feng-ge¹ WANG Zhi-yong^1*

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Abstract

Objective To develop a feasible method to reduce the rat intestinal autofluorescence (AF). Methods Five rats were sacrificed. After cryostat section, intestinal AF was investigated by immunofluorescence (IF) before and after Sudan black B (SBB) treatment. Results The AF within the different rat intestinal segments was easily detected in both FITC- and TRITC-channel spectrum, which was not affected by serum blocking. After 0.3% SBB treatment for 10 minutes, a significant reduction of AF was observed in the rat intestinal tissue. Moreover, the SBB treatment did not affect the expression of CD45 in the rat intestine detected by IF staining, but reduced the AF remarkedly. Conclusion The AF in rat intestinal frozen section can be effectively reduced with 0.3% SBB treatment for 10 minutes.

Key words

Intestine / Autofluorescence / Sudan black B / Immunofluorescence / Rat

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LI Yong ZHOU Li ZHANG Yang LIU Zhen YAO Nian SHEN Feng-ge WANG Zhi-yong. Sudan black B reducing the intestinal autofluorescence of rats and its application in immunofluorescence[J]. Acta Anatomica Sinica. 2020, 51(3): 450-455 https://doi.org/10.16098/j.issn.0529-1356.2020.03.024

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