Preparation and biocompatibility of acellular matrix grafts of human foreskin

doi:10.16098/j.issn.0529-1356.2020.03.023

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (3) : 446-449. DOI: 10.16098/j.issn.0529-1356.2020.03.023

Bioengineering

Preparation and biocompatibility of acellular matrix grafts of human foreskin

LIN Jun-shan¹ ZHAO Xing² LI Du-miao¹ XU Ya-li^1*

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Abstract

Objective To prepare human foreskin tissue acellular matrix graft(AMGs), and explore the toxicity of the extracts on rabbit urethral mucosal cells. Methods After preparing the decellularized matrix of human foreskin tissue, the characteristics of decellularized matrix were observed by Masson staining, and the pore size was observed by scanning electron microscope.

Results Masson staining showed that the cells were removed, and the scaffold was composed of collagen. Scanning electron microscopy showed that the pore size of the scaffold was about 20-50 μm, which was uniformly distributed. The appropriate concentration of AMGs matrix extract had no obvious toxicity to urethral mucosal cells. Conclusion The acellular matrix of human foreskin tissue is an ideal AMGs scaffold material. The prepared AMGs material has low cytotoxicity and has practical value.

Key words

Acellular matrix graft / Urethral mucosa cell / Biocompatibility / Scanning electron microscopy / Human

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LIN Jun-shan ZHAO Xin LI Du-miao XU Ya-li. Preparation and biocompatibility of acellular matrix grafts of human foreskin[J]. Acta Anatomica Sinica. 2020, 51(3): 446-449 https://doi.org/10.16098/j.issn.0529-1356.2020.03.023

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