Effect of PHLDA2 on proliferation, migration, invasion and apoptosis in hepatocellular carcinoma cells

MA Zhan; JIANG Zheng; JIANG Zhong-Xiang

doi:10.16098/j.issn.0529-1356.2020.03.014

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (3) : 398-404. DOI: 10.16098/j.issn.0529-1356.2020.03.014

Effect of PHLDA2 on proliferation, migration, invasion and apoptosis in hepatocellular carcinoma cells

MA Zhan¹ JIANG Zhong-xiang² JIANG Zheng^1*

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Abstract

Objective To observe the expression of pleckstrin homology like domain family A member 2 (PHLDA2) in hepatocellular carcinoma (HCC) and investigate the effects of PHLDA2 on cell proliferation, migration, invasion and apoptosis. Methods To analyze the expression of PHLDA2 in 369 cases of HCC tissues and 160 cases of adjacent normal tissues and the effect of PHLDA2 expression on overall survival rate of HCC patients by gene expression profiling interactive analysis (GEPIA) online database. Cell proliferation was determined by cell counting kit-8 (CCK-8) and colony formation assay. Invasion and migration were detected by Transwell assay. The percentage of apoptosis was measured by flow cytometry. The levels of Bax and cleaved Caspase-3 were measured by Western blotting. Results The expression of PHLDA2 was upregulated in HCC, and high expression of PHLDA2 reduced the overall survival of HCC patients. Low-expression of PHLDA2 inhibited proliferation, invasion and migration, and increased apoptosis of HCC cells. Conclusion PHLDA2 promotes the occurrence and development and may act as a tumor promoter in HCC.

Key words

PHLDA2 / Hepatocellular carcinoma / Proliferation / Colony formation / Flow cytometry

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MA Zhan JIANG Zhong-xiang JIANG Zheng. Effect of PHLDA2 on proliferation, migration, invasion and apoptosis in hepatocellular carcinoma cells[J]. Acta Anatomica Sinica. 2020, 51(3): 398-404 https://doi.org/10.16098/j.issn.0529-1356.2020.03.014

References

［1］ El-Serag HB. Hepatocellular carcinoma［J］. N Engl J Med, 2011, 365(12): 1118-1127.

［2］ Villanueva A, Llovet JM. Liver cancer in 2013: Mutational landscape of HCC-the end of the beginning［J］. Nat Rev Clin Oncol, 2014, 11(2): 73-74.

［3］ Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in Globocan 2012［J］. Int J Cancer, 2015, 136(5): E359-E386.

［4］ Baran Y, Subramaniam M, Biton A, et al. The landscape of genomic imprinting across diverse adult human tissues［J］. Genome Res, 2015, 25(7): 927-936.

［5］ Mohindra V, Tripathi RK, Yadav P, et al. Hypoxia influences expression profile of pleckstrin homology-like domain, family A, member 2 in Indian catfish, Clarias batrachus (Linnaeus, 1758): a new candidate gene for hypoxia tolerance in fish［J］. J Biosci, 2014, 39(3): 433-442.

［6］ Lv Y, Dai H, Yan G, et al. Downregulation of tumor suppressing STF cDNA 3 promotes epithelial-mesenchymal transition and tumor metastasis of osteosarcoma by the Wnt/GSK-3β/β-catenin/Snail signaling pathway［J］. Cancer Letters, 2016, 373(2): 164-173.

［7］ Wang X, He H, Zhang K, et al. The expression of TSSC3 and its prognostic value in patients with osteosarcoma［J］. Biomed Pharmacother, 2016, 79: 23-26.

［8］ Moon H, Oh K, Lee J, et al. Prognostic and functional importance of the engraftment-associated genes in the patient-derived xenograft models of triple-negative breast cancers［J］. Breast Cancer Res Treat, 2015, 154(1): 13-22.

［9］ Idichi T, Seki N, Kurahara H, et al. Molecular pathogenesis of pancreatic ductal adenocarcinoma: Impact of passenger strand of pre-miR-148a on gene regulation［J］. Cancer Sci, 2018, 109(6): 2013-2026.

［10］ Yan GN, Tang XF, Zhang XC, et al. TSSC3 represses self-renewal of osteosarcoma stem cells and Nanog expression by inhibiting the Src/Akt pathway［J］. Oncotarget, 2017, 8(49): 85628-85641.

［11］ Dai J, Lin X, Guo MY, et al. Effect and molecular mechanism of trefoil factor family 3 on the cell cycle of thyroid papillary carcinoma cell［J］. Acta Anatomica Sinica, 2019, 50(2): 211-219.(in Chinese)

代金，林旭，郭梦姚，等. 三叶因子3对甲状腺乳头状癌细胞周期的影响及机制［J］. 解剖学报, 2019, 50(2): 211-219.

［12］ Xue ShH, Lu ZhY, Zhang TCh, et al. Expression and mechanism of micro RNA-940 in breast cancer［J］. Acta Anatomica Sinica, 2019, 50(1): 56-62.(in Chinese)

薛世航，陆振一，张同成，等. MicroRNA-940在乳腺癌中的表达变化及作用机制［J］. 解剖学报, 2019, 50(1): 56-62.

［13］ Goldgar DE, Easton DF, Cannon-Albright LA, et al. Systematic population-based assessment of cancer risk in first-degree relatives of cancer probands［J］. J Natl Cancer Inst, 1994, 86(21): 1600-1608.

［14］ Cohen JH, Kristal AR, Stanford JL. Fruit and vegetable intakes and prostate cancer risk［J］. J Natl Cancer Inst, 2000, 92(1): 61-68.

［15］ Bianchini F, Kaaks R, Vainio H. Overweight, obesity, and cancer risk［J］. Lancet Oncol, 2002, 3(9): 565-574.

［16］ Kolonel LN, Altshuler D, Henderson BE. The multiethnic cohort study: exploring genes, lifestyle and cancer risk［J］. Nat Rev Cancer, 2004, 4(7): 519-527.

［17］ Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells［J］. J Clin Invest, 2008, 118(12): 3917-3929.

［18］ Fresno VJ, Casado E, de Castro J, et al. PI3K/Akt signalling pathway and cancer［J］. Cancer Treat Rev, 2004, 30(2): 193-204.

［19］ Yang J, Weinberg RA. Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis［J］. Dev Cell, 2008, 14(6): 818-829.

［20］ Takao T, Asanoma K, Tsunematsu R, et al. The maternally expressed gene Tssc3 regulates the expression of MASH2 transcription factor in mouse trophoblast stem cells through the Akt-Sp1 signaling pathway［J］. J Biol Chem, 2012, 287(51): 42685-42694.