Effects of microRNAs on invasion, migration and apoptosis of lung cancer cell lines

HAN Jing-Jun; ZHANG Yan-Wei

doi:10.16098/j.issn.0529-1356.2020.03.013

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (3) : 392-397. DOI: 10.16098/j.issn.0529-1356.2020.03.013

Cancer Biology

Effects of microRNAs on invasion, migration and apoptosis of lung cancer cell lines

HAN Jing-jun¹ ZHANG Yan-wei^2*ZHANG Lei¹ XU Ying-jie¹LI Hai-zhou¹ LIU Shu-guang³

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Abstract

Objective To investigate the effects of microRNAs(miRNAs) on invasion, migration and apoptosis of lung cancer cell lines.

Methods Target cells were infected with hsa-mir-933, hsa-mir-4700-3p, hsa-mir-3144-3p, hsa-mir-3972, and hsa-mir-548a-5p. Cell apoptosis was measured using flow cytomertry, Transwell invasion assay,cell migration were analyzed by lineation,miRNA was quantified using Real-time PCR. Results Compared with the vector and control, in hsa-mir-933, hsa-mir-4700-3p, hsa-mir-3144-3p, hsa-mir-397 and hsa-mir-548a-5p group the apoptosis significantly increased,cell invasion ability significantly decreased,cell migration ability significantly decreased, miRNAs expression significantly increased. Conclusion Five microRNAs can promote the apoptosis of lung cancer cell lines, change the high invasiveness of lung cancer cell lines, inhibit the migration of lung cancer cell lines, and increase the expression of corresponding miRNAs.

Key words

MicroRNA / Lung cancer cell line / Invasion / Migration / Real-time PCR

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HAN Jing-jun ZHANG Yan-wei ZHANG Lei XU Ying-jie LI Hai-zhou LIU Shu-guang. Effects of microRNAs on invasion, migration and apoptosis of lung cancer cell lines[J]. Acta Anatomica Sinica. 2020, 51(3): 392-397 https://doi.org/10.16098/j.issn.0529-1356.2020.03.013

References

［1］ Calin GA, Croce CM. MicroRNA signatures in human cancers ［J］.Nat Ｒev Cancer, 2006, 6(11): 857-866.

［2］ Ambros V. The functions of animal microRNAs ［J］. Nature,2004, 431(7006): 350-355.

［3］ Yang WD, Wang H, Wang J. miRNAs: a new potential biomarker for tumor diagnosing and therapying ［J］. Journal of Modern Oncology, 2013, 21(8): 1873-1879.

［4］ Kaduthanam S, Gade S, Meister M, et al. Serum miR-142-3p is associated with early relapse in operable lung adenocarcinoma patients［J］. Lung Cancer, 2013, 80(2):223-227.

［5］ Liu XL, Wang H, Tian X, et al. Correlation between mirna-142-3p expression and prognosis in non-small cell lung cancer tissues［J］. Modern Cancer Medicine, 2017, 25(7): 1051-1053. (in Chinese)

刘新莉,王慧,田雪,等.非小细胞肺癌组织miRNA-142-3p表达及预后的相关性［J］.现代肿瘤医学, 2017, 25(7): 1051-1053.

［6］ Li ChF, Jing W, Xiang DSh, et al. Expression of microrna-613 in human glioma and its effect on proliferation and metastasis ［J］. Acta Anatomica Sinica, 2018, 49(5):579-583. (in Chinese)

李传芬, 荆文, 向冬生, 等. MicroRNA-613在人胶质瘤中的表达及其对增殖与转移的影响［J］. 解剖学报, 2018, 49(5):579-583.

［7］ Deng XJ, Gong ShX, Yang ShH, et al. Targeted regulation of E2F transcription factor 3 inhibits the occurrence and development of liver cancer ［J］. Acta Anatomica Sinica, 2018,49(4):469-474. (in Chinese)

邓学军, 龚邵新, 杨胜辉, 等. MicroRNA-539靶向调控E2F转录因子3抑制肝癌的发生与发展［J］. 解剖学报, 2018,49(4):469-474.

［8］ UI Hussain M. Micro-RNAs (miRNAs): genomic organisation, biogenesis and mode of action［J］. Cell Tissue Res, 2012, 349(2):405-413.

［9］ Taylor MA, Schiemann WP. Therapeutic opportunities for targeting microRNAs in cancer［J］. Mol Cell Ther, 2014, 2(30): 1-13.

［10］ Zhu Z, Zhang X, Wang G, et al. Role of microRNAs in hepatocellular carcinoma［J］. Hepat Mon, 2014, 14(8):e18672.

［11］ Lin L. Study on targets of microRNA and human papillomavirus type 16 ［D］. Fudan University,2013. (in Chinese)

林琳. microRNA与人乳头瘤病毒16型的作用靶点研究［D］.复旦大学,2013.

［12］ Luo ZhP. Role and molecular mechanism of mir-548c-3p in the proliferation, migration and invasion of osteosarcoma cells ［D］. Southern Medical University,2016. (in Chinese)

罗展鹏. miR-548c-3p在骨肉瘤细胞增殖、迁移和侵袭中的作用和分子机制研究［D］.南方医科大学,2016.

［13］ Zhang Y, Ma Y, Xu W, et al. Association of microRNA-933 variant with the susceptibility to gastric cancer［J］. J BUON, 2017, 22(2):390-395.