Dynamic expression of insulin-like growth factor 2 during critical period of visual development in lateral geniculate body of mice

LUO Shi-Shi; SU Qiang; SUN Qiu-Min; XU Jing; CAO Wen-Yu; XU Yang; LIU Zheng-Hai; WAN Wei

doi:10.16098/j.issn.0529-1356.2020.03.005

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (3) : 338-343. DOI: 10.16098/j.issn.0529-1356.2020.03.005

Dynamic expression of insulin-like growth factor 2 during critical period of visual development in lateral geniculate body of mice

LUO Shi-shi¹ SU Qiang¹ SUN Qiu-min² XU Jing¹ CAO Wen-yu¹ XU Yang³ LIU Zheng-hai^1* WAN Wei^1*

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Abstract

Objective To detect the dynamic expression of insulin-like growth factor 2 (IGF2) in lateral geniculate body(LGB) during the critical period of visual development. Methods Three groups of Kunming mice of different ages were selected for testing, which were 3 weeks old, 5 weeks old and 7 weeks old, twelve in each group. The forepaw-reaching reflex test was used to detect whether the visual function of the mice was normal in each group. Immunohistochemical technique was used to detect the expression of IGF2 protein and its receptor in the lateral geniculate body of normal mice at week 3, 5 and 7 postnatal, and to analyze the expression of the protein of IGF2 and its receptor in each part of the lateral geniculate body. Results The expression of IGF2 protein in the dorsal lateral geniculate nucleus decreased significantly at week 5 postnatal and increased significantly at week 7 postnatal, and increased gradually over time at week 5 and week 7 postnatal in the ventral geniculate nucleus. The expression of IGF2 receptor protein in the dorsal lateral geniculate nucleus and ventral nucleus increased significantly at week 5 postnatal, and at week 7 postnatal, the expression of IGF2 receptor decreased to week 3 level in lateral geniculate body of mice. Conclusion The expression of IGF2 and its receptor in lateral geniculate body of mice during critical period of visual development changed dynamically, and the expression patterns of IGF2 and its receptor in different parts of LGB were not completely consistent. The expression of IGF2 and its receptors may be related to the plasticity of visual development in mice.

Key words

Visual critical period / Lateral geniculate body / Insulin-like growth factor 2 / Neuroplasticity / Immunohistochemistry / Mouse

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LUO Shi-shi SU Qiang SUN Qiu-min XU Jing CAO Wen-yu XU Yang LIU Zheng-hai WAN Wei. Dynamic expression of insulin-like growth factor 2 during critical period of visual development in lateral geniculate body of mice[J]. Acta Anatomica Sinica. 2020, 51(3): 338-343 https://doi.org/10.16098/j.issn.0529-1356.2020.03.005

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