Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai

WANG Ru; ZHUANG Wen-Ting; WANG Xiang-Lin; LI Hong-Rong; LI Chang-Xing; KONG De-Xia; LI Jian-Hua

doi:10.16098/j.issn.0529-1356.2020.02.024

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 294-299. DOI: 10.16098/j.issn.0529-1356.2020.02.024

Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai

WANG Ru¹ ZHUANG Wen-ting¹WANG Xiang-lin² LI Hong-rong³ LI Chang-xing¹ KONG De-xia⁴ LI Jian-hua^1*

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Abstract

Objective To investigate the relationship between angiotensin converting enzyme (ACE) and angiotensinogen (AGT) gene expression, gene polymorphism and pregnancy-induced hypertension in Qinghai. Methods A total of 210 pregnant hypertensive patients (HDCP group) and 220 normal pregnant women (CK group) were enrolled. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect AGT M235T and ACE I/D gene polymorphism. Results The proportions of ACE gene DD, ID, and Ⅱ in CK group were 28.15%, 47.73%, and 24.09%, respectively. The HDCP group was 33.81%, 51.90%, and 14.29%, respectively (P<0.05). The frequency distribution of ACE I/D polymorphic alleles I and D was different between HDCP group and CK group(P<0.05). D allele frequency was higher in HDCP group than in CK group (χ2=5.188, P<0.05). ACE genotype distribution was accorded with Hardy-Weinberg genetic balance(χ2=0.423, df=2, check table χ2 boundary value table, P>0.05, reach genetic balance). The proportions of MM, MT and TT in CK group was 24.09%, 43.64% and 32.27%, respectively. The HDCP group was 15.71%, 42.86%, and 41.43% respectively (P<0.05). HDCP group and CK group AGT M235T polymorphic alleles M and T frequency distribution were different (P<0.05). HDCP group T allele frequency was higher than the CK group(χ2=6.796, P<0.05). AGT gene were genotype distribution accorded with Hardy-Weinberg genetic balance (χ2=3.242, df=2, check table χ2 boundary value table, P>0.05, reaching genetic balance). Conclusion The ACE I/D polymorphism and AGT M235T polymorphism are associated with hypertensive disorder of pregnancy in Han nationality in Qinghai Province. D allele and T allele may be susceptible genes of pregnancy-induced hypertension.

Key words

Qinghai / Angiotensin-converting enzyme / Angiotensinogen / Pregnancy-induced hypertension / Gene polymorphism / Polymerase chain reaction-restriction fragment length polymorphism / Pregnant woman

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WANG Ru ZHUANG Wen-ting WANG Xiang-lin LI Hong-rong LI Chang-xing KONG De-xia LI Jian-hua. Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai[J]. Acta Anatomica Sinica. 2020, 51(2): 294-299 https://doi.org/10.16098/j.issn.0529-1356.2020.02.024

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