Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels

HUANG Yan-Yun; HUANG Hua-Tuo; LU Yu-Lan; WEI Ye-Sheng; LEI Ming; TAN Tan

doi:10.16098/j.issn.0529-1356.2020.02.022

PDF(1473 KB)

Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 284-288. DOI: 10.16098/j.issn.0529-1356.2020.02.022

Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels

HUANG Yan-yun^1,2 HUANG Hua-tuo¹ LU Yu-lan¹ LEI Ming¹ TAN Tan¹ WEI Ye-sheng^1*

Author information +

History +

Abstract

Objective To study the distribution characteristics of single nucleotide polymorphisms (SNP) of miR-107 gene rs2296616 C/T in Guangxi healthy population and comparison with that in different ethnic populations, and further to explore the correlation between rs2296616 C/T SNP and blood lipid level. Methods The polymorphisms of miR-107 gene rs2296616C/T among 372 Chinese healthy individuals of Guangxi were detected by multiplex SNaPshot and DNA sequencing method , and the blood lipidrelated indexes were detected by 7600 biochemical analyzer. The distribution of rs2296616 C/T polymorphism among different ethnic groups and the differences of blood lipid levels among different genotypes were compared by statistical method . Results MiR-107 gene rs2296616 C/T SNP contained TT（9.1%）, CT（8.9%）genotypes and T（95.6%）, C（4.4%）alleles in Guangxi healthy population. The frequencies of genotype and allele distribution of rs2296616 C/T were not significantly different among genders in Guangxi population（P>0.05）. However, there were significant differences in the genotype and allele frequency of miR-107 gene rs2296616 C/T in Guangxi healthy population compared with those of Europeans, Japanese, Africans, Mexicans and Indians published in HapMap(P<0.05), no significant difference compared with HapMap-HGB(P>0.05). When compared the blood lipid level among two genotypes in rs2296616 C/T, we found that the level of high density lipoprotein cholesterol(HDL-C) with TT genotype was significantly different from that of CT group(P<0.05). Conclusion There are different degrees of variation in the polymorphisms of rs2296616 C/T of miR-107 gene between Guangxi people and other ethnic populations. The polymorphism of rs2296616 C/T locus is related to the level of HDL-C.

Key words

MiR-107 gene / Single nucleotide polymorphism / rs2296616 C/T / Ethnic / Lipoprotein / Multiplex SNaPshot / DNA sequencing / Guangxi population

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

HUANG Yan-yun HUANG Hua-tuo LU Yu-lan LEI Ming TAN Tan WEI Ye-sheng. Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels[J]. Acta Anatomica Sinica. 2020, 51(2): 284-288 https://doi.org/10.16098/j.issn.0529-1356.2020.02.022

References

［1］ Luo Z, Zheng Y, Zhang W. Pleiotropic functions of miR107 in cancer networks［J］. Onco Targets Ther, 2018, 11: 4113-4124.

［2］ Bhatia H, Pattnaik BR, Datta M. Inhibition of mitochondrial beta-oxidation by miR-107 promotes hepatic lipid accumulation and impairs glucose tolerance in vivo［J］. Int J Obes (Lond), 2016, 40(5): 861-869.

［3］ Gao ZF, Ji XL, Gu J, et al. microRNA-107 protects against inflammation and endoplasmic reticulum stress of vascular endothelial cells via KRT1-dependent Notch signaling pathway in a mouse model of coronary atherosclerosis［J］. J Cell Physiol, 2019, 234(7): 12029-12041.

［4］ Altintas O, Ozgen Altintas M, Kumas M, et al. Neuroprotective effect of ischemic preconditioning via modulating the expression of cerebral miRNAs against transient cerebral ischemia in diabetic rats［J］. Neurol Res, 2016, 38(11): 1003-1011.

［5］ Yang ZB, Zhang Z, Li TB, et al. Up-regulation of brain-enriched miR-107 promotes excitatory neurotoxicity through down-regulation of glutamate transporter1 expression following ischaemic stroke［J］. Clin Sci (Lond), 2014, 127(12): 679-689.

［6］ Wang XL, Zhao DX, Zhuang WT, et al. Association between genetic polymorphism and pregnancy induced hypertension in Qinghai ［J］. Acta Anatomica Sinica, 2018, 49 (3): 379-386.(in chinese)

王香林, 赵得雄, 庄文婷, 等. 基因多态性与青海妊娠高血压疾病的相关性［J］. 解剖学报, 2018, 49(3): 379-386.

［7］ Zhu ShSh, Li SF. Association between D6S296, D8S264 polymorphism and schizophrenia of Han/Zhuang population in Guangxi ［J］. Acta Anatomica Sinica, 2014,45 (2): 286-290(in chinese)

朱莎莎, 李松峰. D6S296、D8S264基因座多态性与广西地区汉/壮族人群精神分裂症的关联性［J］. 解剖学报, 2014, 45(2): 286-290.

［8］ Wang S, Lü C, Jin H, et al. A common genetic variation in the promoter of miR-107 is associated with gastric adenocarcinoma susceptibility and survival［J］. Mutat Res, 2014, 769: 35-41.

［9］ Kroliczewski J, Sobolewska A, Lejnowski D, et al. microRNA single polynucleotide polymorphism influences on microRNA biogenesis and mRNA target specificity［J］. Gene, 2018, 640: 66-72.

［10］ Wu J, Jiang R. Prediction of Deleterious Nonsynonymous SingleNucleotide Polymorphism for Human Diseases［J］. Sci World J, 2013, 2013: 675851.

［11］ Locke JM, Lango Allen H, Harries LW. A rare SNP in pre-miR-34a is associated with increased levels of miR-34a in pancreatic beta cells［J］. Acta Diabetol, 2014, 51(2): 325-329.

［12］ Han BW, Li ZH, Liu SF, et al. A comprehensive review of microRNA-related polymorphisms in gastric cancer［J］. Genet Mol Res, 2016, 15(2). doi: 10.4238/gmr.15028289.

［13］ Ghanbari M, Ikram MA, de Looper HW, et al. Genome-wide identification of microRNA-related variants associated with risk of Alzheimer’s disease［J］. Sci Rep, 2016, 6: 28387.

［14］ Wang W, Jiang B, Sun H, et al. Prevalence, incidence, and mortality of stroke in China: results from a nationwide population-based survey of 480 687 adults［J］. Circulation, 2017, 135(8): 759-771.

［15］ Rahman R, Asombang AW, Ibdah JA. Characteristics of gastric cancer in Asia［J］. World J Gastroenterol, 2014, 20(16): 4483-4490.

［16］ Meng S, Cao J, Wang L, et al. MicroRNA 107 partly inhibits endothelial progenitor cells differentiation via HIF-1beta［J］. PLoS One, 2012, 7(7): e40323.

［17］ Li Y, Chang S, Li W, et al. cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice［J］. Stem Cell Res Ther, 2018, 9(1):139.

［18］ Ruan C, Shen Y, Chen R, et al. Endothelial progenitor cells and atherosclerosis［J］. Front Biosci (Landmark Ed), 2013, 18: 1194-1201.