Effects of picropodophyllin on proliferation and cell cycle of human colorectal cancer HCT-15 cells and its mechanism#br#

ZHOU Li-Li; WANG Rui-Ting; SHEN Xing-Bin; ZHAO Yang; XUE Jing; LIU Sai-Xuan

doi:10.16098/j.issn.0529-1356.2020.02.014

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 233-238. DOI: 10.16098/j.issn.0529-1356.2020.02.014

Effects of picropodophyllin on proliferation and cell cycle of human colorectal cancer HCT-15 cells and its mechanism#br#

ZHOU Li-li¹ WANG Rui-ting² ZHAO Yang¹ XUE Jing³ LIU Sai-xuan¹ SHEN Xing-bin^1*#br#

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Abstract

Objective To investigate the effect of picropodophyllin(PPP) on proliferation and cell cycle of human colorectal cancer cell line HCT-15 and to clarify the related mechanism. Methods Different concentrations of PPP were used to treat HCT-15 cells, the proliferative activity of HCT-15 cells was detected by cell courting kit-8(CCK-8). Morphological changes of HCT-15 cells were observed under inverted microscope. Flow cytometry was used to detect the changes of HCT-15 cell cycle and Western blotting was used to detect the expression of proliferating cell nuclear antigen (PCNA) and cell cyclinD1. Results The result of CCK-8 assay showed that PPP inhibited the proliferation of HCT-15 cells in a dose-time-dependent manner. Under the inverted microscope, it was found that the HCT-15 cells lost their original shape and became round, the refractive index decreased, and the number of living cells decreased significantly. Flow cytometry showed that the proportion of G₂/M cells increased significantly, and the proportion of G₀/G₁ phase and S phase cells decreased significantly. Western Blot result showed that the expression of PCNA, cyclinD1 protein was significantly decreased. Conclusion PPP significantly inhibited the proliferation of human colorectal cancer HCT-15 cells, suggesting that the mechanism might be to block the cells in G₂/M phase by down-regulating the expression of PCNA, cyclinD1 protein.

Key words

Picropodophyllin / Colorectal cancer cells HCT-15 / Flow cytometry / Western blotting / Human

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ZHOU Li-li WANG Rui-ting ZHAO Yang XUE Jing LIU Sai-xuan SHEN Xing-bin. Effects of picropodophyllin on proliferation and cell cycle of human colorectal cancer HCT-15 cells and its mechanism#br#[J]. Acta Anatomica Sinica. 2020, 51(2): 233-238 https://doi.org/10.16098/j.issn.0529-1356.2020.02.014

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