Trichostatin A promotes the migration of esophageal squamous carcinoma cells by protein kinase C signaling pathway#br#

LIU Dan-Hui; LIU Yu-Zhen; CHEN Yan-Min; CHEN Zhi; ZHAO Bao-Sheng

doi:10.16098/j.issn.0529-1356.2020.02.013

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 228-232. DOI: 10.16098/j.issn.0529-1356.2020.02.013

Cancer Biology

Trichostatin A promotes the migration of esophageal squamous carcinoma cells by protein kinase C signaling pathway#br#

LIU Dan-hui^1,2 LIU Yu-zhen^1,2,3 CHEN Yan-min^1,2CHEN Zhi^1,2 ZHAO Bao-sheng^1,2*

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Abstract

Objective To investigate the effect of histone deacetylase inhibitor trichostatin A (TSA) on the migration of human esophageal squamous carcinoma cells(ESCC) and the possible mechanism. Methods KYSE-150 cells and EC9706 cells were cultured and Transwell assay was performed to detect the role of TSA alone and combined with protein kinase C (PKC) inhibitor AEB071 on cell migration; the images of morphology after cells treatment with TSA or combination of AEB071 with TSA; Western blotting was conducted to examine the protein level of epithelial-mesenchymal transition (EMT) related signaling molecules. Results TSA promoted the migration of ESCC cells significantly, and PKC inhibitor AEB071 partly inhibited the effect of TSA-promoted ESCC cells migration. Treatment with TSA resulted in the cell morphology transitioned from epithelia oval-like to mesenchymal spindle-like, indicating the EMT. AEB071 partially rescued ESCC cells morphological changes which TSA induced. Western blotting showed that TSA reduced the expression of E-cadherin and augmented the expression of vimentin, β-catenin, Slug and acH3, whereas AEB071 obviously blocked the EMT-related protein level changes which induced by TSA. Conclusion TSA promotes ESCC cells migration via inducing EMT process and the mechanism may be mediated by PKC signaling pathway.

Key words

Histone deacetylase inhibitor / Trichostatin A / Protein kinase C / Epithelial-mesenchymal transition / Esophageal squamous carcinoma cells / Western blotting / Human

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LIU Dan-hui LIU Yu-zhen CHEN Yan-min CHEN Zhi ZHAO Bao-sheng. Trichostatin A promotes the migration of esophageal squamous carcinoma cells by protein kinase C signaling pathway#br#[J]. Acta Anatomica Sinica. 2020, 51(2): 228-232 https://doi.org/10.16098/j.issn.0529-1356.2020.02.013

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