Inhibitory effect of recombinant human semaphorin 3A on angiogenesis of gastric cancer and the associated mechanisms#br#

FENG Pin; FAN Wen-Jing; LIU Lei; XU Qian; LI Yu-Hong; ZUO Yan-Zhen; ZHOU Bo; ZHAO Xiang-Yang

doi:10.16098/j.issn.0529-1356.2020.02.012

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 220-227. DOI: 10.16098/j.issn.0529-1356.2020.02.012

Inhibitory effect of recombinant human semaphorin 3A on angiogenesis of gastric cancer and the associated mechanisms#br#

FENG Pin¹ FAN Wen-jing¹ LIU Lei² XU Qian³ LI Yu-hong⁴ ZUO Yan-zhen⁵ ZHOU Bo⁶ ZHAO Xiang-yang^7*

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Abstract

Objective To investigate the expression of semaphorin 3A (Sema3A) and its receptor neuropilin-1 (NRP-1) in gastric cancer and its correlation with microvessel density (MVD)，and then to explore the effect of recombinant human Sema3A on angiogenesis of gastric cancer and the associated mechanisms. Methods Forty cases of gastric cancer tissues and its corresponding adjacent normal tissues were used to detecte the expression of Sema3A, NRP-1 and MVD in tissues by immunohistochemistry method . The expression level of Sema3A in serum of gastric cancer patient group and normal control group were measured by Enzyme-linked immuno-sorbent assay (ELISA). Western blotting was used to detect the expression of Sema3A and NRP-1 in five gastric cancer cell lines (MGC-803,HGC-27,MKN-28,SGC-7901,MKN-45) and human gastric mucosal epithelial cell (GES-1). Transwell chamber was used to construct non-contact in vitro co-culture system, in which the effects of different concentrations of recombinant human Sema3A on angiogenesis in gastric cancer were analyzed by tube formation assay preliminarily. The expression levels of vascular endothelial growth factor receptor 2 (VEGFR2) and NRP-1 in co-culture system were detected by Western blotting. Results The expression levels of Sema3A in gastric cancer tissues, cell lines and patient serum were significantly lower than that in the control group（P<0.05）, while the expression of NRP-1 in gastric cancer tissues and MKN-28 cells was significantly increased, and both of them were associated with TNM staging of gastric cancer（P<0.05）. In vitro co-culture system, The tube forming abilities of human umbilical vein endothelial cell (HUVEC) were decreased in recombinant human Sema3A treated group, and this phenomenon was concentration dependent. The expression of VEGFR2 protein was down-regulated by recombinant human Sema3A. Conclusion The expression of Sema3A was decreased in gastric cancer tissues, cell lines and patient serum, and negatively correlated with microvessel density. The recombinant human Sema3A could inhibit the angiogenesis of gastric cancer in vitro, which may be related to down-regulation of VEGFR2 protein expression.

Key words

Gastric cancer / / Semaphorin3A / / Umbilical vein endothelial cell / / Co-culture system / Tube formation assay / / Western blotting / / Human

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FENG Pin FAN Wen-jing LIU Lei XU Qian LI Yu-hong ZUO Yan-zhen ZHOU Bo ZHAO Xiang-yang. Inhibitory effect of recombinant human semaphorin 3A on angiogenesis of gastric cancer and the associated mechanisms#br#[J]. Acta Anatomica Sinica. 2020, 51(2): 220-227 https://doi.org/10.16098/j.issn.0529-1356.2020.02.012

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