Antagonism of resveratrol on oxidative stress injury of follicular granulosa cells

XING Peng; ZHANG Na

doi:10.16098/j.issn.0529-1356.2020.02.007

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 189-194. DOI: 10.16098/j.issn.0529-1356.2020.02.007

Cell and Molecules Biology

Antagonism of resveratrol on oxidative stress injury of follicular granulosa cells

XING Peng^1,2 ZHANG Na^1* ZHAO Jing-yun¹ZHANG Yi¹ Lü Cui-ting¹ XU Bi-sha¹

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Abstract

Objctive To investigate the protective effect of resveratrol on oxidative stress damage of follicular granulosa cells induced by hydrogen peroxide. Methods Granulosa cells were collected from the follicular fluid of in vitro fertilization(IVF) patients after oocyte retrieval and cultured. The cultured granulosa cells were divided into four groups: control group, injury model group, 10 μmol/L resveratrol group and 50 μmol/L resveratrol group. Cell viability was determined by CCK-8 assay, malondialdehyde(MDA) content by thiobarbituric(TBA) assay, superoxide dismutase(SOD) level by water soluble tetrazdium-1(WST-1) assay, apoptosis by AnnexinV-FITC/PI double-staining flow cytometry, Bcl-2 and Caspase-3 protein expression by Western blotting, and progesterone secretion by competitive ELISA. Resutls Compared with the control group, the cell viability, SOD level, Bcl-2 protein expression and progesterone secretion were significantly decreased in the injury model group, while MDA content, apoptosis rate and Caspase-3 apoptotic protein expression were significantly increased (P<0.05). Compared with the injury model group, the 10 μmol/L resveratrol group showed no statistically significant differences in various parameters; however, the cell viability, SOD level, progesterone secretion, and Bcl-2 and silent information regulator factor 2 related enzyme 1(SIRT1) protein expression were significantly increased, and the MDA content, apoptosis rate, and Caspase-3 apoptotic protein expression were significantly decreased in the 50 μmol/L resveratrol group (P<0.05). Conclusion 50 μmol/L resveratrol can increase the activity of SIRT1, enhance the anti-oxidation and anti-apoptosis ability of granulosa cells and improve the function of granulosa cells.

Key words

Resveratrol

/ Hydrogen peroxide / Follicular granulosa cells / Oxidative stress / Antioxidants / Western blotting / Human

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XING Peng ZHANG Na ZHAO Jing-yun ZHANG Yi Lü Cui-ting XU Bi-sha. Antagonism of resveratrol on oxidative stress injury of follicular granulosa cells[J]. Acta Anatomica Sinica. 2020, 51(2): 189-194 https://doi.org/10.16098/j.issn.0529-1356.2020.02.007

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