Protective effect of Rutin on myelin toxicity in the corpus callosum of rats induced by acrylamide

JIA Hui

doi:10.16098/j.issn.0529-1356.2020.02.006

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 184-188. DOI: 10.16098/j.issn.0529-1356.2020.02.006

Neurobiology

Protective effect of Rutin on myelin toxicity in the corpus callosum of rats induced by acrylamide

JIA Hui ZHANG Chun-mei GU Zi-ting LI Shi-qi LUO Li MA Yu-xin LIU Jing*

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Abstract

Objective To study the effect of rutin（Rut）on the expression of myelin basic protein (MBP) and myelin protein lipoprotein (PLP) in corpus callosum of rats infected with acrylamide(ACR). Methods Thirty-two SD adult male rats were randomly divided into 4 groups：control，20 mg/kg acrylamide poisoning group (ACR), 100 mg/kg Rut protection group (R1+ACR), 200 mg/kg Rut protection group (R2+ACR)，8 in each group，and were given gastric gavage for 21 days. The changes of the rats’ gait were recorded weekly; Immunohistochemistry and Western blotting were used to detect the changes in the expression levels of MBP and PLP in each group of rats. Results The gait score results showed that the gait score of the ACR group increased with the extension of exposure time compared with the control group. The gait score of the R1+ACR group and R2+ACR group also showed an increase trend compared with the control group, but the gait score was significantly lower than that of the ACR group (P<0.05). Immunohistochemistry and Western blotting results showed that the expression of MBP and PLP in the corpus callosum of the ACR group was significantly decreased compared with the control group (P<0.01), while the expression of MBP and PLP in the R1+ACR group and R2+ACR group increased (P<0.05). Conclusion Rutin has a protective effect on myelin sheath in rats infected with acrylamide, which may be related to the inhibition of MBP and PLP in corpus callosum induced by ACR infection.

Key words

Acrylamide / Rutin / Corpus callosum / Myelin basic protein / Myelin sheath protein lipoprotein / Immunohistochemistry / Western blotting / Rat

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JIA Hui ZHANG Chun-mei GU Zi-ting LI Shi-qi LUO Li MA Yu-xin LIU Jing. Protective effect of Rutin on myelin toxicity in the corpus callosum of rats induced by acrylamide[J]. Acta Anatomica Sinica. 2020, 51(2): 184-188 https://doi.org/10.16098/j.issn.0529-1356.2020.02.006

References

［1］ Erkekoglu P, Baydar T. Acrylamideneurotoxicity ［J］. Nutr Neurosci, 2014, 17(2):49-57.

［2］ Harry GJ. Acrylamide-induced alterations in axonal transport ［J］. Mol Neurobiol, 1992, 6(2-3):203-216.

［3］ Prineas J. The pathogenesis of dying-back polyneuropathies ［J］. Neuropathol ExpNeurol, 1969, 28(4): 571-597.

［4］ Jangir BL, Mahaprabhu R, Rahangadale S, et al. Neurobehavioral alterations and histopathological changes in brain and spinal cord of rats intoxicated with acrylamide ［J］. Toxicol Ind Health, 2016, 32(3):526-540. 

［5］ Parzefall W. Minireview on the toxicity of dietary acrylamide ［J］. Food Chem Toxicol, 2018, 46(4):1360-1364.

［6］ Zhang LJ, Yang LQ, Wang PP, et al. Research progress on toxicity of acrylamide ［J］. Chinese Journal of Food Science, 2018, 18(8):274-283. (in Chinese)

张璐佳, 杨柳青, 王鹏璞，等. 丙烯酰胺毒性研究进展［J］. 中国食品学报, 2018, 18(8):274-283.

［7］ Budzynska B, Faggio C, Kruk-Slomka M, et al. Rutin as neuroprotective agent:from bench to bedside［J］. Curr Med Chem, 2019,26(27):5152-5164

［8］ Li L, Sun HY, Liu W, et al. Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells ［J］. Food Chem Toxicol, 2017, 102:93-101.

［9］ O’Sullivan SA, Velasco-Estevez M, Dev KK. Demyelination induced by oxidative stress is regulated by sphingosine 1-phosphate receptors ［J］. Glia, 2017, 65(7):1119-1136.

［10］ Motamedshariaty VS, Amel Farzad S, Nassiri-Asl M, et al. Effects of rutin onacrylamide-inducedneurotoxicity ［J］. Daru, 2014, 22(1):27.

［11］ Yang DH, Lai ShM, Liu J, et al. Effect of acrylamide poisoning on expression of myelin basic protein and myelin protein in corpus callosum of rats during pregnancy and feeding period ［J］. Health Research, 2017, 46(5):843-846. (in Chinese)

杨德慧, 赖胜敏, 刘靖，等.丙烯酰胺染毒对孕哺期大鼠胼胝体髓鞘碱性蛋白和髓鞘蛋白脂蛋白表达的影响［J］.卫生研究,2017, 46(5):843-846.

［12］ Bishnoi M, Chopra K, Kulkarni SK. Protective effect of rutin, a polyphenolic flavonoid against haloperidol-induced orofacial dyskinesia and associated behavioural, biochemical and neurochemical changes ［J］. Fundam Clin Pharmacol, 2007, 21(5):521-529.

［13］ Han Sh, Li YH, Zhou YD, et al. Research progress on neuronal axon signal regulating myelin sheath development ［J］. Progress in Physiological Sciences, 2018, 49(3):181-186. (in Chinese)

韩硕, 李云鸿, 周煜东，等. 神经元轴突信号调节髓鞘发育的研究进展［J］. 生理科学进展, 2018, 49(3):181-186.

［14］ Gruenenfelder FI, Thomson G, Penderis J, et al. Axon-glial interaction in the CNS: what we have learned from mouse models of Pelizaeus-Merzbacher disease ［J］. J Anat, 2011, 219(1):33-43.

［15］ Saab AS, Nave KA. A mechanism for myelin injury ［J］. Nature, 2016, 529(7587):474-475.

［16］ Doyle S, Hansen DB, Vella J, et al. Vesicular glutamate release from central axons contributes to myelin damage ［J］. Nat Commun, 2018, 9(1):1032.

［17］ Wang Y, Sun P, Wang Q, et al. Differentiation and quantification of inflammation, demyelination and axon injury or loss in multiple sclerosis ［J］. Brain, 2015, 138(5):1223-1238.

［18］ Caprariello AV, Mangla S, Miller RH, et al. Apoptosis of oligodendrocytes in the central nervous system results in rapid focal demyelination［J］. Ann Neurol, 2012, 72(3): 395-405.

［19］ Perfeito R, Pereira J, Oliveira CR, et al. Trolox protection of myelin membrane in hydrogen peroxide-treated mature oligodendrocytes ［J］. Free Radic Res, 2007, 41(4):444-451.

［20］ Gruber RC, Rocca D, Minchenberg SB, et al. The control of reactive oxygen speci-es production by SHP-1 in oligodendrocytes ［J］. Glia, 2015, 63(10):1753-1771.

［21］ O’Sullivan SA, Velasco-Estevez M, Dev KK. Demyelination induced by oxidative stress is regulated by sphingosine 1-phosphate receptors ［J］. Glia,2017,65(7):1119-1136.

［22］ Yu XL, Li YN, Zhang H, et al. Rutin inhibits amylin-induced neurocytotoxicity and oxidative stress ［J］. Food Funct, 2015, 6(10):3296-3306.

［23］ Ahmed MM, Hussein MMA. Neurotoxic effects of silver nanoparticles and the protective role of rutin ［J］. Biomed Pharmacother, 2017, 90:731-739.