Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland

FAN Ya-Nan; LI Chao-Hong; JIA Xiang-Lei; LIU Yu-Zhen

doi:10.16098/j.issn.0529-1356.2020.02.002

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (2) : 162-166. DOI: 10.16098/j.issn.0529-1356.2020.02.002

Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland

FAN Ya-nan LI Chao-hong JIA Xiang-lei LIU Yu-zhen*

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Abstract

Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in rat adrenal gland and to detect the effect of cyclic intermittent hypoxia (CIH) on the expression of BACE1. Methods The expression and localization of BACE1 in rat adrenal gland were detected by Western blotting and immunohistochemistry. Sixteen male Sprague-Dawley (SD) rats were randomly divided into two groups: control group and CIH group, 8 rats in each group. The protein levels of BACE1 and tyrosine hydroxylase (TH) in rat adrenal medulla were detected by Western blotting after CIH 2 weeks treatment. Results BACE1 was mainly localized in rat adrenal medullary nerve fibers. Compared with the control group, BACE1 protein level decreased and TH protein level increased in the adrenal medulla in the CIH group.

Conclusion BACE1 is located in rat adrenal medullary nerve fibers. The decreased level of BACE1 may participate in slowing down the excessive enhancement of sympathetic activity induced by CIH.

Key words

β-site amyloid precursor protein cleaving enzyme 1 / Adrenal gland / Tyrosine hydroxylase / Cyclic intermittent hypoxia / Western blotting / Rat

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FAN Ya-nan LI Chao-hong JIA Xiang-lei LIU Yu-zhen. Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland[J]. Acta Anatomica Sinica. 2020, 51(2): 162-166 https://doi.org/10.16098/j.issn.0529-1356.2020.02.002

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