Enhancing effect of Lupeol on migration and invasion abilities of ST3GalⅢ-silenced MDA-MB-231 cells#br#
&nbsp;#br#

WANG Ming

doi:10.16098/j.issn.0529-1356.2020.01.005

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (1) : 26-31. DOI: 10.16098/j.issn.0529-1356.2020.01.005

Cancer Biology

Enhancing effect of Lupeol on migration and invasion abilities of ST3GalⅢ-silenced MDA-MB-231 cells#br# #br#

WANG Ming CUI Hong-xia LIU Ji-cheng*

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Abstract

Objective In this study, we expored the enhancing effect of Lupeol on migration and invasion abilities of ST3GalⅢ-silenced MDA-MB-231 cells. Methods Human breast cancer cell line ST3GalⅢ -silenced MDA-MB-231 was cultured in vitro. The cell adhesion, Transwell and woud healing test were utilized to test the effect of Lupeol on ST3GalⅢ-silenced MDA-MB-231 cells. The expression of matrix metalloproteinase(MMP)-2, -9, phosphatidylinositol 3-kinase/protein kinase B/nuclear factor κB(PI3K/Akt/NF-κB) signaling pathway were examined by Western blotting. Results No significant influence of the decreased expression of ST3GalⅢ and Lupeol（5μmol/L）on proliferation and apoptosis of MDA-MB-231 cells were found. Lupeol inhibited the migration and invasion of ST3GalⅢ-silenced MDA-MB-231 cells in vitro(P<0.05). Furthermore, the expression of NF-κB p65, p-Akt, Akt, p85, MMP-2 and MMP-9 levels were significantly down-regulated. Conclusion These observations suggest that Lupeol may inhibit the abilities of migration and invasion of ST3GalⅢ-silenced MDA-MB-231 cells in vitro by inhibiting the protein expression of MMP-2, -9 and effect the signaling pathway of PI3K/Akt/NF-κB.

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WANG Ming CUI Hong-xia LIU Ji-cheng. Enhancing effect of Lupeol on migration and invasion abilities of ST3GalⅢ-silenced MDA-MB-231 cells#br# #br#[J]. Acta Anatomica Sinica. 2020, 51(1): 26-31 https://doi.org/10.16098/j.issn.0529-1356.2020.01.005

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