Role of hippocampal sortilin in streptozocin inducing diabetic cognitive impairment mice

LUO Dan; YANG Hui; LUO Shi-Shi; NIU Lei; LI Wei; SONG Guang-Ming; XIE Liang-Zhe; XU Yang; CAO Wen-Yu; HE Jie

doi:10.16098/j.issn.0529-1356.2020.01.002

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Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (1) : 9-14. DOI: 10.16098/j.issn.0529-1356.2020.01.002

Neurobiology

Role of hippocampal sortilin in streptozocin inducing diabetic cognitive impairment mice

LUO Dan¹ YANG Hui¹ LUO Shi-shi² NIU Lei² LI Wei² SONG Guang-ming² XIE Liang-zhe² XU Yang³ CAO Wen-yu^2* HE Jie^1*

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Abstract

Objective To investigate the role of hippocampal sortilin in streptozotocin (STZ) inducing diabetic cognitive impairment in mice.

Methods Twenty four adult male ICR mice were randomly divided into control group (NS group) and experimental group (STZ group). The animal model of diabetic cognitive impairment was induced by STZ treatment. The blood glucose was measured by blood glucose meter every other week. The cognitive function was determined by novelty object recognition test 8 weeks after STZ injection. The expressions of sortilin in hippocampus of mice were determined by immunohistochemistry. The expressions of sortilin and brain-derived neurotrophic factor (BDNF）protein in hippocampus of mice were determined by Western blotting. The expressions of sortilin and BDNF mRNA in hippocampus of mice were determined by Real-time PCR. Results STZ treated mice showed significantly increased blood glucose compared with the NS group (P<0.01). Compared with the NS group, the STZ group showed significantly reduced discrimination index in the novelty object recognition test (P<0.05). The result of immunohistochemistry assay showed that the positive score of NS group was significantly higher than STZ group (P<0.05).The results of Western blotting and Real-time PCR showed that the expression level of sortilin protein (P<0.01) and mRNA (P<0.05) in the hippocampus of STZ group decreased significantly compared with the NS group. The results of Western blotting and Real-time PCR showed that the expression level of BDNF protein (P<0.05) and mRNA (P<0.01) in the hippocampus of STZ group decreased significantly compared with the NS group.Conclusion The STZ-induced cognitive impairment in mice may be related to the down-regulation of sortilin in the hippocampus.

Key words

Streptozotocin / Hippocampus / Diabetes / Cognitive impairment / Sortilin / Immunohistochemistry / Mouse

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LUO Dan YANG Hui LUO Shi-shi NIU Lei LI Wei SONG Guang-ming XIE Liang-zhe XU Yang CAO Wen-yu HE Jie. Role of hippocampal sortilin in streptozocin inducing diabetic cognitive impairment mice[J]. Acta Anatomica Sinica. 2020, 51(1): 9-14 https://doi.org/10.16098/j.issn.0529-1356.2020.01.002

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