Objective To explore the feasibility and effects of teaching with tissue construction thinking of organ in histology and embryology. Methods One hundred twenty students from four year 2017 clinical major classes in Mudanjiang medical university, who participated in the study of histology and embryology, were consecutively selected. Traditional teaching mode which is lecture-base learning was used in the control class，while the experimental class was taught by tissue construction thinking of organ,the evaluations were taken at the end，including questionnaires and exam scores of four classes. Results The experimental class was distinctly superior to the control class in learning interest, innovation ability, self-study ability, manipulative ability,psychological quality and team spirit (P＜0.01）, and in exam scores also (P＜0.05）. Conclusion Tissue construction thinking of organ is particulary adapted to the teaching of histology and embryology.The thinking can play a vital role in resolving the problems in teaching of Histology and embryology. And it will also help to cultivate high-quality applied medical talents with innovative ability.