Preparation and identification of thyroid gland decellularized biologic scaffold

WENG Jie HUANG Tao-tao WANG Zhi-yi* CHEN Chan WAN Xin-long ZHOU Xiao-ming MEI Jin WANG Zhi-bin

doi:10.16098/j.issn.0529-1356.2019.06.022

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (6) : 827-830. DOI: 10.16098/j.issn.0529-1356.2019.06.022

Bioengineering

Preparation and identification of thyroid gland decellularized biologic scaffold

WENG Jie¹HUANG Tao-tao² WANG Zhi-yi^1,3,4* CHEN Chan⁵ WAN Xin-long³ ZHOU Xiao-ming⁴ MEI Jin³ WANG Zhi-bin³

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Abstract

Objective To prepare thyroid gland decullularized biologic scaffolds by soaking with rotating and to assess the decullularized scaffolds. Methods According to random number table method, twenty New Zealand white rabbits were divided into control group and decullularized group, 10 cases in each group. The thyroid glands in decullularized group were immersed in 1% SDS and rotated at 37 ℃ for 24 hours. All thyroid gland decullularized scaffolds were rotated in deionized water to wipe off the decullularized reagent and residual DNA. HE staining, DNA concentration examination, electron microscope analysis and immunofluorescence technique were performed to indentify the characteristics of decullularized scaffolds. Results Compared to control group, the DNA concentration in the decellularized scaffold decrease significantly (P<0.01), the removal rate of DNA exceeded 90%; there were no visible cell nuclei remained, the 3D space structure and the protein components preserved well. Conclusion Soaking and rotating method can effectively wipe off the cellular components from the thyroid gland tissue, and preserve the extracellular matrix and three-dimensional structure.

Key words

Decullularization / Thyroid gland / Extracellular matrix / Immunofluorescence / Rabbit

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WENG Jie HUANG Tao-tao WANG Zhi-yi CHEN Chan WAN Xin-long ZHOU Xiao-ming MEI Jin WANG Zhi-bin. Preparation and identification of thyroid gland decellularized biologic scaffold[J]. Acta Anatomica Sinica. 2019, 50(6): 827-830 https://doi.org/10.16098/j.issn.0529-1356.2019.06.022

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