Effect of sodium glycosaminodeoxycholate on apoptosis of hepatic stem cells in cholestatic microenvironment

LI Zhi-peng WANG Jian XIAO Cheng KANG Quan* LUO Qing

doi:10.16098/j.issn.0529-1356.2019.06.008

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (6) : 747-753. DOI: 10.16098/j.issn.0529-1356.2019.06.008

Cell and Molecules Biology

Effect of sodium glycosaminodeoxycholate on apoptosis of hepatic stem cells in cholestatic microenvironment

LI Zhi-peng ^1,2 WANG Jian ^1,2 XIAO Cheng ^1,2 KANG Quan ^1* LUO Qing²

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Abstract

Objective To investigate the effects of hepatic stem cells survival in the pathological microenvironment of cholestatic cirrhosis, and the effects of sodium glycosaminodeoxycholate (GCDC) on apoptosis of hepatic stem cells in vitro. Methods Balb/c mice were subjected to bile duct ligation (BDL)to simulate the pathological environment of cholestatic cirrhosis；Liver stem cells HP14-19 were transplanted back into liver by the splenic vein and survival of cell colonization was detected；Effects of sodium glycosaminodeoxycholate on the viability of HP14-19 cells at different concentrations by cell counting kit-8(CCK-8) and crystal violet staining, flow cytometry was used to detect the apoptosis of HP14-19 cells treated with 600 μmol/L GCDC 24 hours later, and the expression of Bax，Bcl-2，Capase-3，phosphorylated adenosine 5’-monophosphate-activated protein kinase α(p-AMPKα) and adenosine 5’-monophosphate-activated protein kinase α(AMPKα) were detected by Western blotting. Results The results of CCK-8 and crystal violet staining showed that the proliferation of HP14-19 cells was inhibited with the increase of the concentration of sodium glycosaminodeoxycholate. Flow cytometry showed that the apoptosis rate of GCDC treated group was higher than that of control group. Compared with the control group, the expression of Bax, Capase-3 was up-regulated and the expression of Bcl-2 protein was down-regulated in the experimental group. The results showed that GCDC could induce apoptosis of HP14-19 (P<0.05) AMPK was activated. Conclusion Microenvironment of cholestatic cirrhosis induced apoptosis of liver stem cells.

Key words

Cholestatic cirrhosis / Sodium glycosaminodeoxycholate / Liver stem cell / Western blotting / Mouse

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LI Zhi-peng WANG Jian XIAO Cheng KANG Quan LUO Qing. Effect of sodium glycosaminodeoxycholate on apoptosis of hepatic stem cells in cholestatic microenvironment[J]. Acta Anatomica Sinica. 2019, 50(6): 747-753 https://doi.org/10.16098/j.issn.0529-1356.2019.06.008

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