Dose-effect relationship between L-glutamic acid and duodenal movement in the vagus pathway

ZHANG Jin ZHANG Ying-li LI Xiang DU Yi-nan ZHAO Jian-shuai ZHANG Liu-ning XU Yong-ping*

doi:10.16098/j.issn.0529-1356.2019.06.004

PDF(197 KB)

Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (6) : 722-728. DOI: 10.16098/j.issn.0529-1356.2019.06.004

Neurobiology

Dose-effect relationship between L-glutamic acid and duodenal movement in the vagus pathway

ZHANG Jin¹ ZHANG Ying-li¹ LI Xiang² DU Yi-nan¹ZHAO Jian-shuai¹ZHANG Liu-ning² XU Yong-ping^1*

Author information +

History +

Abstract

Objective To establish a mathematical model of the injection by dorsal motor nucleus of vagus(DMV), the dose of L-glutamic acid（L-Glu）and the duodenal myoelectric activity index of female rabbits was established to provide a theoretical basis for studying the mechanism of action of hormones, cytokines and other vagal activity. Methods In this experiment, we used microinjection technique to inject L-Glu to DMV by doses of 0 mol/L, 0.05 mol/L, 0.10 mol/L, 0.15 mol/L and 0.20 mol/L,monitoring the duodenal myoelectric activity of ovarian abducted rabbits,the number of samples per dose group was 5, and construct a mathematical model between the dose of L-Glu and the index of duodenal myoelectric activity. Results After t-test analysis, the amplitude of myoelectric activity increased gradually between adjacent dose groups, and the difference was extremely significant (P<0.01).The frequency was gradually strengthened, and the difference between 0 mol/L and 0.05 mol/L, 0.10 mol/L and 0.15 mol/L was significant (P<0.05).The index of myoelectric activity increased gradually, and the difference between groups was extremely significant (P<0.01). With L-Glu concentration as the independent variable x, the duodenal myoelectric activity index was the dependent variable y, the constructor relationship was y=13.71/1 + exp ［-22.35 × (x-0.082)］, function fitting accuracy was R²=0.9948，P<0.01，belonging to growth type S logic function. Function analysis showed that the L-Glu dose of 0.082 mol/L was the inflection point of the logic function. As the dose range of L-Glu was 0 mol/L 0.082 mol/L,duodenal myoelectric activity index showed an exponential growth pattern,as the L-Glu dose was greater than 0.082 mol/L,the duodenal myoelectric activity index showed a logarithmic growth pattern with a theoretical limit of 13.71. Conclusion L-Glu has a significant dose-effect relationship on the promotion of duodenal myoelectric activity in rabbits by DMV, and have the effect of interval,the mathematical model laid the theoretical foundation for further research on the role of hormones and cytokines on this basis.

Key words

L-glutamic acid / Dorsal motor nucleus of the vagus / Myoelectric activity index / Mathematical model / Duodenum / Stereotactic method of brain / Female rabbit

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHANG Jin ZHANG Ying-li LI Xiang DU Yi-nan ZHAO Jian-shuai ZHANG Liu-ning XU Yong-ping. Dose-effect relationship between L-glutamic acid and duodenal movement in the vagus pathway[J]. Acta Anatomica Sinica. 2019, 50(6): 722-728 https://doi.org/10.16098/j.issn.0529-1356.2019.06.004

References

［1］ Simth IJ, Kellett DO, Jordan D, et al. Oxytocin-immunoreactive innervation of identifiedneurons in the rat dorsal vagal complex［J］. Neurogastroenterol Motil, 2012, 24(3):136-14.

［2］ Bülbül M, Babygirija R, Zheng J, et al. Central orexin-A changes the gastrointestinal motor pattern from interdigestive to postprandial in rats［J］. Auton Neurosci, 2010, 158(1-2):24-30.

［3］ Fontán JJ, Diec CT, Velloff CR, et al. Bilateral distribution of vagal motor ad sensory nerve fibers in the rat's lungs and airways［J］. Am J Physiol Regul Integr Comp Physiol, 2000, 279(2):713-728.

［4］ Paterson DS, Darnall R. 5-HT2A receptors are concentrated in regions of the human infant medulla involved in respiratory and autonomic control［J］. Auton Neurosci, 2009, 147(1):48-55.

［5］ Machhada A, Ang R, Ackland GL, et al. Control of ventricular excitability by neurons of the dorsal motor nucleus of the vagus nerve［J］. Heart Rhythm, 2015, 12(11):2285-2293.

［6］ Talman WT, Robertson SC, Cassell MD, et al. Mechanisms of cardiovascular responses to glycine injected into the dorsal vagal motor nucleus in rat［J］. Hypertension, 1992, 19(2):187-192.

［7］ Mussa BM, Verberne AJ. The dorsal motor nucleus of the vagus and regulation of pancreatic secretory function［J］.Exp Physiol,2013,98(1):25-37.

［8］ Sun HZ, Yu KN, Ai HB. Role of hydrogen sulfide within the dorsal motor nucleus of the vagus in the control of gastric function in rats［J］.Neurogastroenterol Motil,2015,27(5):618-626.

［9］ Yoon SH, Sim SS, Hahn SJ, et al. Stimulatory role of the dorsal motor nucleus of the vagus in gastrointestinal motility through myoelectromechanical coordination in cats ［J］. J Auton Nerv Syst, 1996, 57(2): 22-28.

［10］ Wang J, Liu J, Liu C, et al. Effects of nitric oxide in the dorsal motor nucleus of the vagus on the extrahepatic biliary system in rabbits［J］. Auton Neuroscience, 2001, 87(1):46-51.

［11］ Cruz MT, Murphy EC, Sahibzada N, et al. A reevaluation of the effects of stimulation of the dorsal motor nucleusof the vagus on gastric motility in the rat［J］. Am J Physiol Regul Integr Comp Physiol, 2007, 292(1):R291-307.

［12］ Sun N, Liu J, Qi W, et al. Effects of the vagus nerve dorsal nucleus on the electromyographic activity of the digestive small intestine ［J］. Journal of Neuroanatomy, 2014, 30(5):524-528. (in Chinese)

孙娜, 刘江, 齐巍,等. 迷走神经背核对消化间期小肠肌电活动的影响［J］. 神经解剖学杂志, 2014, 30(5):524-528.

［13］ Zhang XY. Comparative study on the effects of rat nucleus and vagus dorsal nucleus on gastric acid and bicarbonate secretion ［D］. Shandong Normal University, 2004. (in Chinese)

张学英. 大鼠疑核和迷走神经背核对胃酸、碳酸氢盐分泌影响的比较研究［D］. 山东师范大学,2004.

［14］ Li H, He T, Xu Q, et al. Acupuncture and regulation of gastrointestinal function ［J］. World J Gastroenterol, 2015, 21(27):8304-8313.

［15］ Browning KN, Travagli RA. Central nervous system control of gastrointestinal motility and secretion and modulation of gastrointestinal functions ［J］. Compr Physiol, 2014, 4(4):1339-1368.

［16］ Guo X, Li Q, Chen WD, et al. Expression of progesterone receptors in the cervical and thoracic ganglia of female goats［J］.Acta Anatomica Sinica,2014,45(4):465-468.(in Chinese)

郭晓,李强,陈文东,等. 孕激素受体在雌性山羊颈胸神经节的表达［J］. 解剖学报,2014,45(4):465-468.

［17］ Dong W, Li Q, Xu YP, et al.Distribution of gonadotropin-releasing hormone receptor in cervical and thoracic ganglia of female goats［J］.Acta Anatomica Sinica,2014,45(2):166-170.(in Chinese)

董伟,李强,徐永平,等. 促性腺激素释放激素受体在雌性山羊颈胸神经节中的分布［J］. 解剖学报, 2014,45(2):166-170.

［18］ Sun M, Jin XF, Huang Sh, et al. Expression of γ-interferon receptor in carotid body of goat［J］. Chinese Journal of Anatomy, 2016, 39(5):568-571.(in Chinese)

孙曼,金秀芳,黄双,等. γ-干扰素受体在山羊颈动脉体的表达［J］. 解剖学杂志, 2016,39(5):568-571.

［19］ Du YN. Mechanism of estradiol on the electrical activity of duodenum in female rabbits ［D］. Northwest A&F University, 2018.(in Chinese)

杜宜楠. 雌二醇对雌性家兔十二指肠肌电活动作用的机制研究［D］. 西北农林科技大学, 2018.

［20］ Palomba S, Dicello A, Riccio E, et al. Ovarian function and gastrointestinal motor activity［J］. Minerva Endocrinol, 2011, 36(4):295-310.

［21］ Bouyou J, Gaujoux S, Marcellin L, et al. Abdominal emergencies during pregnancy ［J］. J Visc Surg, 2015, 152(6):105-115.

［22］ Matos JF, Americo MF, Sinzato YK, et al. Role of sex hormones in gastrointestinal motility in pregnant and non-pregnant rats［J］. World J Gastroenterol, 2016, 22(25): 5761-5768.

［23］ He F, Ai HB. Effects of electrical stimulation at different locations in the central nucleus of amygdala on gastric motility and spike activity［J］. Physiol Res 2016, 65(4): 693-700.

［24］ Cao Q, Qian XJ, Zhao LZh, et al. Observation of the reduction of intragastric pressure by nucleus of the vagus nerve and the reversal of the paraventricular nucleus［J］. Journal of Anhui Medical University, 1994, 29(1): 22-24. (in Chinese)

曹琦, 钱雪景, 赵乐章, 等. 损毁迷走神经背核翻转室旁核降低胃内压的观察［J］. 安徽医科大学学报, 1994,29(1): 22-24.

［25］ Krowicki ZK, Arimura A, Nathan NA, et al. Hindbrain effects of PACAP on gastric motor function in the rat ［J］. Am J Physiol, 1997, 272(5): 1221-1229.

［26］ Ni LH, Xie CW, Wu DP, et al. Prediction of the effects of different immunization strategies on the pathogenesis of varicella using mathematical models［J］. South China Journal of Preventive Medicine, 2018, 44(3): 261-264.(in Chinese)

倪莉红, 谢才文, 吴德平, 等. 运用数学模型预测不同免疫策略对水痘发病的影响［J］. 华南预防医学, 2018, 44(3): 261-264.

［27］ Tang R, Jiang ZhL, Xie YC, et al. Application of mathematical model to screen coronary heart disease ［J］. Chinese Prescription Drugs, 2017, 15(11): 1-3. (in Chinese)

唐蓉, 江志兰, 谢永财, 等. 应用数学模型筛查冠心病的研究［J］. 中国处方药, 2017, 15(11): 1-3.

［28］ Zhang R, Kavana M.Quantitative analysis of receptor allosterism and its implication for drug discovery ［J］. Expert Opin Drug Discov, 201510(7): 763-780.