Effects of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression

YAN Li WANG Jing YONG Jun XIAO Hong-mei ZENG Qing-fan*

doi:10.16098/j.issn.0529-1356.2019.06.003

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (6) : 718-721. DOI: 10.16098/j.issn.0529-1356.2019.06.003

Effects of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression

YAN Li WANG Jing YONG Jun XIAO Hong-mei ZENG Qing-fan*

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Abstract

Objective To evaluate the role of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression. Methods Neurons were separated and purificated and identificated by microtubule associated protein-2 (MAP-2) antibody. Rats were divided into normal control group, propofol group(add 12 mg/L propofol), and propofol + dexmedetomidine group(add 12 mg/L propofol and 0.25 mg/L dexmedetomidine). The expression of Bcl-2 test by Real-time PCR and Western blotting; cell synapse was detected by transmission electron microscope. Results Rat hippocampal neurons were separated successfuly. Hippocampal nerve cells in control group were given priority to with multiple processes of pyramidal cell, the cell body was triangular or elliptic, large cell body bright and pervious to light quality, had a halo around, abundant cytoplasm, nuclear. Compared with the control group, in propofol group the Bcl-2 expression decreased obviously. Compared with the propofol group, the expression of Bcl-2 increased obviously (P<0.05). Conclusion Dexmedetomidine can influence the expression of Bcl-2 so as to play a protective role of hippocampal neurons.

Key words

Propofol / Dexmedetomidine / Bcl-2 / Hippocampal neuron / Real-time PCR / Rat

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YAN Li WANG Jing YONG Jun XIAO Hong-mei ZENG Qing-fan. Effects of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression[J]. Acta Anatomica Sinica. 2019, 50(6): 718-721 https://doi.org/10.16098/j.issn.0529-1356.2019.06.003

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