Effect of chronic compression injury of trigeminal nerve root on expression of myelin basic protein and P75 in trigeminal ganglion cells

LIN Ren LUO Li-li LI Qiu-hua CHEN Ting QIU Rong-hui LUO Dao-shu*

doi:10.16098/j.issn.0529-1356.2019.06.001

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (6) : 707-712. DOI: 10.16098/j.issn.0529-1356.2019.06.001

Effect of chronic compression injury of trigeminal nerve root on expression of myelin basic protein and P75 in trigeminal ganglion cells

LIN Ren^1,3 LUO Li-li ^1,2 LI Qiu-hua ^1,2 CHEN Ting^1,2 QIU Rong-hui ^1,3LUO Dao-shu^1,2*

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Abstract

Objective To investigate the effect of the interaction between satellite glial cells and neurons in the trigeminal ganglion of trigeminal neuralgia (TN) rat model.Methods In the TN rat model (n=24) induced by trigeminal root entry zone chronic compression and sham group (n=24), immunofluorescence and Western blotting were used to detect the expression of myelin basic protein (MBP), glial fibrillary acidic protein (GFAP) and of P75 neurotrophic factor receptor (P75NTR) in the trigeminal ganglion. Results The expression of P75NTR in TN group was higher than that in the sham operation group, especially on the post-operation day (POD) 7 and 14 (P<0.01). The expression of MBP in the TN group was decreased after operation, which were significant lower than that in the sham operation group on POD 7 and 14(P<0.05). While the expression of immunoreactive GFAP in the satellite glial cells in the TN group was higher than that in the sham operation group from POD 7 to POD 21 after operation (P<0.01). Conclusion The changes of MBP and P75NTR expression in neurons and the activation of satellite glial cells in trigeminal ganglion of the TN animal model may affect the interaction of neuron-satellite glial cells, which may be involved in the nociceptive information transmission from orofacial area to the central nervous system in the TN rats.

Key words

Trigeminal neuralgia / Trigeminal ganglion / Satellite glial cell / Animal model / Western blotting / Immunofluorescence / Rat

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LIN Ren LUO Li-li LI Qiu-hua CHEN Ting QIU Rong-hui LUO Dao-shu. Effect of chronic compression injury of trigeminal nerve root on expression of myelin basic protein and P75 in trigeminal ganglion cells[J]. Acta Anatomica Sinica. 2019, 50(6): 707-712 https://doi.org/10.16098/j.issn.0529-1356.2019.06.001

References

［1］ Majeed MH, Arooj S, Khokhar MA, et al. Trigeminal neuralgia: a clinical review for the general physician［J］. Cureus, 2018, 10(12): e3750.

［2］ Maarbjerg S, Di Stefano G, Bendtsen L, et al. Trigeminal neuralgia - diagnosis and treatment［J］. Cephalalgia, 2017, 37(7): 648-657.

［3］ Hossain MZ, Unno S, Ando H, et al. Neuron-glia crosstalk and neuropathic pain: involvement in the modulation of motor activity in the orofacial region［J］. Int J Mol Sci, 2017, 18(10): 2051.

［4］ Pannese E, Procacci P. Ultrastructural localization of NGF receptors in satellite cells of the rat spinal ganglia［J］. J Neurocytol, 2002, 31(8-9): 755-763.

［5］ Zhou XF, Rush RA, Mclachlan EM. Differential expression of the p75 nerve growth factor receptor in glia and neurons of the rat dorsal root ganglia after peripheral nerve transection［J］. J Neurosci, 1996, 16(9): 2901-2911.

［6］ Tomita K, Kubo T, Matsuda K, et al. The neurotrophin receptor p75NTR in Schwann cells is implicated in remyelination and motor recovery after peripheral nerve injury［J］. Glia, 2007, 55(11): 1199-1208.

［7］ Zhang H, Ren NT, Zhou FQ, et al. Effects of hindlimb unweighting on MBP and GDNF expression and morphology in rat dorsal root ganglia neurons［J］. Neurochem Res, 2016, 41(9): 2433-2442.

［8］ Luo DS, Zhang T, Zuo CX, et al. An animal model for trigeminal neuralgia by compression of the trigeminal nerve root［J］. Pain Physician, 2012, 15(2): 187-196.

［9］ Pannese E. Biology and pathology of perineuronal satellite cells in sensory ganglia［M］. Germany: Heidelberg, 2018: 1-63.

［10］Chiang CY, Dostrovsky JO, Iwata K, et al. Role of glia in orofacial pain［J］. Neuroscientist, 2011, 17(3): 303-320.

［11］Katagiri A, Shinoda M, Honda K, et al. Satellite glial cell P2Y12 receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats［J］. Mol Pain, 2012, 8(23): 1-8.

［12］Xu M, Aita M, Chavkin C. Partial infraorbital nerve ligation as a model of trigeminal nerve injury in the mouse: behavioral, neural, and glial reactions［J］. J Pain, 2008, 9(11): 1036-1048.

［13］Lin R, Luo L, Gong Y, et al. Immunohistochemical analysis of histone H3 acetylation in the trigeminal root entry zone in an animal model of trigeminal neuralgia［J］. J Neurosurg, 2018, 11(1): 1-11.

［14］Costa FA, Moreira Neto FL. ［Satellite glial cells in sensory ganglia: its role in pain］［J］. Rev Bras Anestesiol, 2015, 65(1): 73-81.

［15］Vassall KA, Bamm VV, Harauz G. MyelStones: the executive roles of myelin basic protein in myelin assembly and destabilization in multiple sclerosis［J］. Biochem J, 2015, 472(1): 17-32.

［16］Ma HY, Yu B, Kong L,et al. Brain derived newotrophic factor gene-modified cortical neural stem cells of rat embryo and their effect on differentiation in vitro［J］. Acat Anatomica Sinica, 2011, 42(1): 70-74.（in Chinese）

马海英，喻博，孔力，等. 脑源性神经营养因子基因修饰的胚胎大鼠皮质神经干细胞及其在体外的分化［J］. 解剖学报, 2011, 42(1): 70-74.

［17］Cosgaya JM, Chan JR, Shooter EM. The neurotrophin receptor p75NTR as a positive modulator of myelination［J］. Science, 2002, 298(5596): 1245-1248.

［18］Saxena S, Howe CL, Cosgaya JM, et al. Differences in the surface binding and endocytosis of neurotrophins by p75NTR［J］. Mol Cell Neurosci, 2004, 26(2): 292-307.

［19］Fukui Y, Ohtori S, Yamashita M, et al. Low affinity NGF receptor (p75 neurotrophin receptor) inhibitory antibody reduces pain behavior and CGRP expression in DRG in the mouse sciatic nerve crush model［J］. J Orthop Res, 2010, 28(3): 279-283.