Perfusion and cryopreservation of whole ovaries with vascular pedicles in common experimental animals

CHEN Wen-ming XU Zhi-hui*

doi:10.16098/j.issn.0529-1356.2019.05.022

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (5) : 672-676. DOI: 10.16098/j.issn.0529-1356.2019.05.022

Perfusion and cryopreservation of whole ovaries with vascular pedicles in common experimental animals

CHEN Wen-ming¹ XU Zhi-hui^2*

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Abstract

Objective Using the perfusion needle which is authorized and special perfusion technology to perfuse the whole ovary with vascular pedicle of common animals, such as rabbits, guinea pigs and rats, then comparative analysis of the adjustable perfusion needles whether can be applied to different animals, whether this kind of perfusion pressure and perfusion rate is appropriate for different sizes of ovaries, and using the classic cryopreservation protocol to freeze and thaw. Methods Collecting 12 ovaries respectively from 6 chinese sexual maturity rabbits, 6 Dunkan-Hartley guinea pigs and 6 SD rats to do experiments. Results Through HE staining to count the normal proportion of primordial follicles in each section, the result of HE staining in three animals indicated that there was no statistical significance (P＞0.05), vascular injury was mainly in the upper (far away from ovary), there was no obvious damage in lower (close to ovary) in three animals’ each group. Conclusion The experiment confirms that through adjusting straw needle size we can perfuse different size of animal organs and under the condition of the same perfusion pressure (60 mmHg) and rate (1 ml/min) it is suitable for rabbits’ ovaries, guinea pigs’ ovaries and rats’ ovaries at the same time.

Key words

Whole ovary / Perfusion / Straw needle / HE staining / Rabbit / Guinea pig / Rat

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CHEN Wen-ming XU Zhi-hui. Perfusion and cryopreservation of whole ovaries with vascular pedicles in common experimental animals[J]. Acta Anatomica Sinica. 2019, 50(5): 672-676 https://doi.org/10.16098/j.issn.0529-1356.2019.05.022

References

［1］ Sivasakthivel T, Lipeng X, Kalyanasundaram R, et al. Glycyrrhizin induces apoptosis in prostate cancer cell lines DU-145 and LNCaP ［J］. Oncol Rep, 2008, 20(6): 1387-1392.

［2］ Silber SJ, Derosa M, Goldsmith S, et al. Cryopreservation and transplantation of ovarian tissue: results from one center in the USA ［J］. J Assist Reprod Genet, 2018, 35(12): 2205-2213.

［3］ Donnez J, Silber S, Andersen CY, et al. Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births ［J］. Ann Med, 2011, 43(6): 437-450.

［4］ Donnez J, Jadoul P, Pirard C, et al. Live birth after transplantation of frozen-thawed ovarian tissue after bilateral oophorectomy for benign disease ［J］. Fertil Steril, 2012, 98(3): 720-725.

［5］ Kim SS, Lee WS, Chung MK, et al. Long-term ovarian function and fertility after heterotopic autotransplantation of cryobanked human ovarian tissue: 8-year experience in cancer patients ［J］. Fertil Steril, 2009, 91(6): 2349-2354.

［6］ Rosendahl M, Andersen CY, Ernst E, et al. Ovarian function after removal of an entire ovary for cryopreservation of pieces of cortex prior to gonadotoxic treatment: a follow-up study ［J］. Hum Reprod, 2008, 23(11): 2475-2483.

［7］ Andersen CY, Rosendahl M, Byskov AG, et al. Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue ［J］. Hum Reprod, 2008, 23(10): 2266-2272.

［8］ Meirow D, Ra’anani H, Shapira M, et al. Transplantations of frozen-thawed ovarian tissue demonstrate high reproductive performance and the need to revise restrictive criteria ［J］. Fertil Steril, 2016, 106(2): 467-474.

［9］ Jensen AK, Kristensen SG, Macklon T, et al. Outcomes of transplantations of cryopreserved ovarian tissue to 41 women in Denmark ［J］. Hum Reprod, 2015, 30(12): 2838-2845. ［10］Silber S. Ovarian tissue cryopreservation and transplantation: scientific implications ［J］. J Assist Reprod Genet, 2016, 33(12): 1595-1603.

［11］Silber SJ, Derosa M, Goldsmith S, et al. Cryopreservation and transplantation of ovarian tissue: results from one center in the USA ［J］. J Assist Reprod Genet, 2018, 35(12): 2205-2213.

［12］Kim SS. Time to re-think: ovarian tissue-transplantation versus whole ovary transplantation ［J］. Reprod Biomed Online, 2010, 20(2): 171-174.

［13］Imhof M, Bergmeister H, Lipovac M, et al. Orthotopic microvascular reanastomosis of whole cryopreserved ovine ovaries resulting in pregnancy and live birth ［J］. Fertil Steril, 2006, 85(Suppl 1)：1208-1215.

［14］Arav A, Gavish Z, Elami A, et al. Ovarian function 6 years after cryopreservation and transplantation of whole sheep ovaries ［J］. Reprod Biomed Online, 2010, 20(1): 48-52.

［15］Bedaiwy MA, Jeremias E, Gurunluoglu R, et al. Restoration of ovarian function after autotransplantation of intact frozen-thawed sheep ovaries with microvascular anastomosis ［J］. Fertil Steril, 2003, 79(3): 594-602.

［16］Ding Y, Shao JL, Li JW, et al. Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model ［J］. J Ovarian Res, 2018, 11(1): 35.

［17］Jin S, Lei L, Shea LD, et al. Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation ［J］. Fertil Steril, 2010, 93(8): 2627-2632.［18］Zhao M, Mydlarz WS, Califano J. Head and neck cancer cell lines are resistant to mitochondrial-depolarization-induced apoptosis ［J］. ORL J Otorhinolaryngol Relat Spec, 2008, 70(4): 257-263.

［19］Courbiere B, Massardier J, Salle B, et al. Follicular viability and histological assessment after cryopreservation of whole sheep ovaries with vascular pedicle by vitrification ［J］. Fertil Steril, 2005, 84 (Suppl 2)：1065-1071.

［20］Xu Z, Wang X, Wu Y, et al. Slow-controlled freezing versus speed-cooling for cryopreservation of whole guinea pig ovaries ［J］. Theriogenology, 2012, 77(3): 483-491.

［21］Yang Ch. Tempromandibular joint surgery ［J］. Chinese Journal of Stomatology, 2004, 16(5): 193-198.（in Chinese）

杨驰. 颞下颌关节外科［J］. 中华口腔医学杂志, 2004, 16(5): 193-198.

［22］Onions VJ, Webb R, Pincott-Allen C, et al. The effects of whole ovarian perfusion and cryopreservation on endothelial cell-related gene expression in the ovarian medulla and pedicle ［J］. Mol Hum Reprod, 2013, 19(4): 205-215.