Comparison of surface markers of mesenchymal stem cells from different sources

DU Zhi-peng YIN Guo-tian LI Miao-miao GUO Zhi-kun LI Qiong*

doi:10.16098/j.issn.0529-1356.2019.05.008

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (5) : 589-594. DOI: 10.16098/j.issn.0529-1356.2019.05.008

Cell and Molecules Biology

Comparison of surface markers of mesenchymal stem cells from different sources

DU Zhi-peng¹ YIN Guo-tian² LI Miao-miao¹ GUO Zhi-kun¹ LI Qiong ^1,3*

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Abstract

Objective To investigate the expression difference of surface markers of human umbilical cord mesenchymal stem cells (HUCMSCs), human adipose mesenchymal stem cells (ADSCs) and human fetal blood source endometrial mesenchymal stem cells (MenSCs) and the changes of surface markers with the culture generation. Methods HUCMSCs, ADSCs and MenSCs were cultured to passage 3, 6, 9 and 12. Five MSC-specific markers, CD29, CD44, CD73, CD90 and CD105, and one HSC markers, CD45, were assessed by flow cytometry and immunofluorescence. Results The cultured MenSCs and HUCMSCs were spindle-shaped, and the ADSCs forms were mainly spindle-shaped and multiple-angular. The results of flow cytometry showed that the MSC-positive markers including CD29, CD44, CD73 and CD105 were highly expressed by at least 95% in the passage 3 HUCMSCs, ADSCs and MenSCs cells and CD45 negative expression. Specifically, CD90 levels of MenSCs of passage 3 was (72.43±0.76)%, which was lower than that in HUCMSCs (99.67±0.12)% and ADSCs (99.70±0.15)%(P<0.001). There was no significant difference in the expression rates of surface markers CD29, CD44, CD73, CD90, CD105 and CD45 with the increase of culture passages in HUCMSCs, ADSCs and MenSCs (P>0.05). The results of immunofluorescence was consistent with those of flow cytometry.Conclusion With the increase of culture time, HUCMSCs, ADSCs and MenSCs are stable in high expression of CD29, CD44, CD73, CD90 and CD105, which does not express CD45, while the CD90 expression rate of MenSCs is lower than that in HUCMSCs and ADSCs.

Key words

Umbilical cord mesenchymal stem cell / Adipose-derived mesenchymal stem cell / Menstrual endometrial mesenchymal stem cell / Immunofluorescence / Flow cytometry / Human

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DU Zhi-peng YIN Guo-tian LI Miao-miao GUO Zhi-kun LI Qiong. Comparison of surface markers of mesenchymal stem cells from different sources[J]. Acta Anatomica Sinica. 2019, 50(5): 589-594 https://doi.org/10.16098/j.issn.0529-1356.2019.05.008

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