Expression of Bcl-2 and Bax after cerebral ischemia-reperfusion injury in rats with hyperlipemia and the effect of scutellarin

YANG Ying-chun* WANG Rui-fang CHEN Xin-ji ZHANG Xiao-liang GAO Sai-hong REN Zhan-chuan

doi:10.16098/j.issn.0529-1356.2019.04.005

PDF(7451 KB)

Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (4) : 431-437. DOI: 10.16098/j.issn.0529-1356.2019.04.005

Expression of Bcl-2 and Bax after cerebral ischemia-reperfusion injury in rats with hyperlipemia and the effect of scutellarin

YANG Ying-chun* WANG Rui-fang CHEN Xin-ji ZHANG Xiao-liang GAO Sai-hong REN Zhan-chuan

Author information +

History +

Abstract

Objective To investigate the effect of scutellarin after cerebral ischemia-reperfusion injury in rats with hyperlipemia,by observing the expression of B-cell lymphoma-2 (Bcl-2) and Bcl-2 assaciated X protein (Bax) in cerebral cortex with hyperlipemia. Methods After the rat model of hyperlipidemia was established, the focal cerebral ischemia-reperfusion model in hyperlipemia rats was established with thread embolism of the middle cerebral artery. The neurobehavioral score was used to observe the symptoms of neurobehavioral injury. 2,3,5 -triphenyl tetrazolium chloride (TTC）staining was used to observe the cerebral infarct volume. HE staining was used to observe the pathological changes of brain tissue. Immunohistochemistry and Western blotting were used to observe the expressions of Bcl-2 and Bax. Results Compared with the sham-operation group, the neurobehavioral scores,cerebral infarction volum and pathologic damage were significantly increased,the expressions of Bcl-2 was significantly lower, the expressions of Bax was significantly increased in ischemiareperfusion group(P<0.05). Compared with the saline group, the neurobehavioral scores, cerebral infarction volum and pathologic damage were significantly decreased,the expression of Bcl-2 was significantly increased, the expression of Bax was significantly decreased in scutellarin treatment group (P<0.05). Conclusion In cerebral ischemia-reperfusion injury rats with hyperlipemia, scutellarin can alleviate cerebral ischemia-reperfusion injury by promoting the expression of Bcl-2 and inhibiting the expression of Bax.

Key words

Hyperlipemia

/ Cerebral ischemia-reperfusion injury / B-cell lymphoma-2 / Bcl-2 assaciated X protein / Scutellarin / Western blotting / Rat

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

YANG Ying-chun WANG Rui-fang CHEN Xin-ji ZHANG Xiao-liang GAO Sai-hong REN Zhan-chuan. Expression of Bcl-2 and Bax after cerebral ischemia-reperfusion injury in rats with hyperlipemia and the effect of scutellarin[J]. Acta Anatomica Sinica. 2019, 50(4): 431-437 https://doi.org/10.16098/j.issn.0529-1356.2019.04.005

References

［1］ Nour M, Scalzo F, Liebeskind DS. Ischemia-reperfusion injury in stroke［J］. Interv Neurol, 2013, 1(3-4): 185-199. 

［2］ Ayata C, Shin HK, Dilek?z E, et al. Hyperlipidemia disrupts cerebrovascular reflexes and worsens ischemic perfusion defect［J］. J Cereb Blood Flow Metab, 2013, 33(6): 954-962.

［3］ Sun K, Fan J, Han J. Ameliorating effects of traditional Chinese medicine preparation, Chinese materia medica and active compounds on ischemia/reperfusion-induced cerebral microcirculatory disturbances and neuron damage［J］. Acta Pharm Sin B, 2015, 5(1): 8-24.

［4］ Shen S, Zhou J, Meng S, et al. The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax ［J］. Exp Ther Med, 2017, 14(5): 4077-4082.

［5］ Jiang Y, Xu H, Wang J. Alantolactone induces apoptosis of human cervical cancer cells via reactive oxygen species generation, glutathione depletion and inhibition of the Bcl-2/Bax signaling pathway ［J］. Oncol Lett, 2016, 11(6): 4203-4207.

［6］ Lu Y, Zhang J, Ma B, et al. Glycine attenuates cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis in mice ［J］. Neurochem Int, 2012, 61(5): 649-658.

［7］ Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without caraniectomy in rats［J］. Stroke, 1989, 20(1):84-91.

［8］ Yu Y, Wu X, Pu J, et al. Lycium barbarum polysaccharide protects against oxygen glucose deprivation/reoxygenation-induced apoptosis and autophagic cell death via the PI3K/Akt/mTOR signaling pathway in primary cultured hippocampal neurons［J］. Biochem Biophys Res Commun, 2018, 495(1):1187-1194.

［9］ Liu F, Jiang YJ, Zhao HJ, et al. Electroacupuncture ameliorates cognitive impairment and regulates the expression of apoptosis-related genes Bcl-2 and Bax in rats with cerebral ischaemia-reperfusion injury［J］. Acupunct Med, 2015, 33(6):478-484.

［10］ Li XJ, Liang L, Shi HX, et al. Neuroprotective effects of curdione against focal cerebral ischemia reperfusion injury in rats［J］. Neuropsychiatr Dis Treat, 2017, 13:1733-1740.

［11］ Guo C, Zhu Y, Weng Y, et al. Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats［J］. J Ethnopharmacol, 2014, 151(1):660-666.

［12］ Chai L, Guo H, Li H, et al. Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocyte［J］. J Ethnopharmacol, 2013, 28;150(1):100-107.

［13］ Zhang HF, Hu XM, Wang LX, et al. Protective effects of scutellarin against cerebral ischemia in rats: evidence for inhibition of the apoptosis-inducing factor pathway［J］. Planta Med, 2009,75(2):121-126.

［14］ Lu J, Cheng C, Zhao X, et al. PEG-scutellarin prodrugs: synthesis, water solubility and protective effect on cerebral ischemia/reperfusion injury［J］. Eur J Med Chem, 2010, 45(5):1731-1738.

［15］ Qian L, Shen M, Tang H, et al. Synthesis and protective effect of scutellarein on focal cerebral ischemia/reperfusion in rats［J］. Molecules, 2012, 17(9):10667-10674.

［16］ Xu XH, Chen Y, Zhen XX. The protective effect of breviscapine on the toxicity of glutamic acid in rat hippocampal nerve cells［J］. Acta Pharmaceutica Sinica, 2007,42(6):538-588. (in Chinese)

徐晓虹，陈瑜，郑筱祥.灯盏花素对谷氨酸诱导大鼠海马神经细胞毒性的保护作用［J］.药学学报，2007,42(6):538-588.

［17］ Liu YH, Lin Bin, Fu XH, et al. Clinical study on the treatment of ischemic cerebral apoplexy with breviscapine injection［J］. Modern Diagnosis and Treatment, 2013,24(3):554-555. (in Chinese)

刘毅华，林斌，付秀华，等.注射用灯盏花素治疗缺血性脑卒中的临床研究［J］.现代诊断与治疗,2013,24（3）：554-555.

［18］ Liu XL, Zhang W, Tang SJ. Intracranial transplantation of human adipose-derived stem cells promotes the expression of neurotrophic factors and nerve repair in rats of cerebral ischemia-reperfusion injury［J］. Int J Clin Exp Pathol, 2014, 7(1): 174-183.

［19］ Schett G, Zwerina J, Firestein G. The p38 mitogen-activated protein kinase(MAPK) pathway in rheumatoid arthritis［J］. Ann Rheum Dis, 2008, 67(7):909-916

［20］ Wang J, Zuo QL, Xu HR. The role of p38 MAPK in focal cerebral ischemia reperfusion injury in rats ［J］. Journal of Practical Clinical Medicine, 2011, 15(13):5-7. (in Chinese)

王军, 左其龙, 徐汉荣.p38 MAPK在大鼠局灶性脑缺血再灌注损伤中的作用［J］.实用临床医药杂志, 2011, 15(13):5-7.

［21］ Liang W, Huang XB, Chen WQ. The Effects of Baicalin and Baicalein on Cerebral Ischemia［J］. Aging Dis, 2017,8(6): 850-867.

［22］ Yang YCh, Ren ZhCh, Liang YG. Study of the expression of cyclooxygenase-2, malonic dialdehyde and the protective effect of Tetramethylpyrazine after cerebral ischemic-reperfusion in rat［J］.Acth Anatomica Sinica, 2009, 40(6):886-890. (in Chinese)

杨迎春, 任占川, 梁勇刚. 大鼠脑缺血再灌注后诱导型环氧合酶和丙二醛的表达及川芎嗪的干预作用［J］. 解剖学报, 2009, 40(6):886-890.

［23］ Liu ZD, Chukwunweike IO, Zhang L, et al. Mixed polyethylene glycol-modified breviscapine-loaded solid lipid nanoparticles for improved brain bioavailability: preparation, characterization, and in vivo cerebral microdialysis evaluation in adult sprague dawley rats［J］. AAPS Pharm Sci Tech, 2014, 15(2): 483-449.