Effect and mechanism of caloric restriction on early social isolation-induced cognitive impairment in mice

NIU Lei LUO Shi-shi ZENG Jia-yu WANG Zhen XU Yang CHEN Xi CAO Wen-yu* WAN Wei*

doi:10.16098/j.issn.0529-1356.2019.04.003

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (4) : 418-422. DOI: 10.16098/j.issn.0529-1356.2019.04.003

Neurobiology

Effect and mechanism of caloric restriction on early social isolation-induced cognitive impairment in mice

NIU Lei¹ LUO Shi-shi¹ ZENG Jia-yu¹ WANG Zhen¹ XU Yang² CHEN Xi¹ CAO Wen-yu^1* WAN Wei^1*

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Abstract

Objective To investigate the effect and mechanism of caloric restriction (CR) on early social isolation (SI) induced cognitive dysfunction in mice. Methods Thirty-six kunming mice (3-weeks old) were randomly divided into group house (GH, n=12), early socially isolated (SI, n=12), caloric restriction and socially isolated (SI+CR, n=12).The GH group (6 mice/cage) and SI group (1 mice/cage) were reared separately under the same conditions, while the SI+CR group (1 mice/cage) were given food every other day. The open field experiment was used to measure the locomotive of mice; The object recognition task was used to evaluate the cognitive function; The expression of microglial marker ionized calcium-binding adapter molecule 1 (IBA-1) in hippocampus was detected by immunohistochemistry. The expression of microglial activation marker CD68 and interleukin-1β (IL-1β) in hippocampus was detected by Western blotting. Results In the open field test, there was no difference in the activity of the three groups of mice. Compared with the GH group, the SI group showed significantly reduced discrimination ratio in object recognition task, with remarkably up-regulated expression of IL-1β in hippocampus (P<0.01). In addition, SI group exhibited over-activated microglia in hippocampus indicated by increased number of IBA-1-positive cells and high expression of CD68 (P<0.01). Compared with the SI group, SI+CR group enhanced discrimination ratio in object recognition task, with down-regulated expression of IL-1β in hippocampus (P<0.01), and the number of IBA-1-positive cells and the expression of CD68 significantly reduced (P<0.01). Conclusion Caloric restriction alleviates SI-induced cognitive dysfunction, which might be attributed to the inhibiting of microglia activation in the hippocampus.

Key words

Early social isolation / Caloric restriction / Cognitive dysfunction / Microglia / Hippocampus / Western blotting / Mouse

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NIU Lei LUO Shi-shi ZENG Jia-yu WANG Zhen XU Yang CHEN Xi CAO Wen-yu WAN Wei. Effect and mechanism of caloric restriction on early social isolation-induced cognitive impairment in mice[J]. Acta Anatomica Sinica. 2019, 50(4): 418-422 https://doi.org/10.16098/j.issn.0529-1356.2019.04.003

References

［1］ Wukitsch TJ, Reinhardt EK, Kiefer SW, et al. Voluntary ethanol consumption during early social isolation and responding for ethanol in adulthood［J］. Alcohol, 2018,77:1-10.

［2］ Hoshino S, Kobayashi M, Higami Y. Mechanisms of the anti-aging and prolongevity effects of caloric restriction: evidence from studies of genetically modified animals［J］. Aging (Albany NY), 2018,10(9):2243-2251.

［3］ Stewart J, Mitchell J, Kalant N. The effects of life-long food restriction on spatial memory in young and aged Fischer 344 rats measured in the eight-arm radial and the Morris water mazes［J］. Neurobiol Aging, 1989,10(6):669-675.

［4］ Talani G, Biggio F, Licheri V, et al. Isolation rearing reduces neuronal excitability in dentate gyrus granule cells of adolescent C57BL/6J mice: role of gabaergic tonic currents and neurosteroids［J］. Front Cell Neurosci, 2016,10：158.

［5］ Ma L, Wang R, Dong W, et al. Caloric restriction can improve learning and memory in C57/BL mice probably via regulation of the AMPK signaling pathway［J］. Exp Gerontol, 2018,102:28-35.

［6］ Tanca A, Abbondio M, Palomba A, et al. Caloric restriction promotes functional changes involving short-chain fatty acid biosynthesis in the rat gut microbiota［J］. Sci Rep, 2018, 8(1):14778.

［7］ Xu Y, Cao W, Zhou M, et al. Inactivation of BRD7 results in impaired cognitive behavior and reduced synaptic plasticity of the medial prefrontal cortex［J］. Behav Brain Res, 2015, 286:1-10.

［8］ Cao WY, Xu Y, Luo YW, et al. Activation of ERK1/2 is required for normal response of isosexual social interactions in male rats［J］. Brain Res, 2013,1538：51-60.

［9］ Yusufishaq S, Rosenkranz JA. Post-weaning social isolation impairs observational fear conditioning［J］. Behav Brain Res, 2013, 242:142-149.

［10］Wang B, Wu Q, Lei L, et al. Long-term social isolation inhibits autophagy activation, induces postsynaptic dysfunctions and impairs spatial memory［J］. Exp Neurol, 2019, 311:213-224.

［11］Beltran-Castillo S, Eugenin J, von Bernhardi R. Impact of aging in microglia-mediated d-serine balance in the CNS［J］. Mediators Inflamm, 2018, 2018:7219732.

［12］Arakawa H. Ethological approach to social isolation effects in behavioral studies of laboratory rodents［J］. Behav Brain Res, 2018, 341:98-108.

［13］Calcia MA, Bonsall DR, Bloomfield PS, et al. Stress and neuroinflammation: a systematic review of the effects of stress on microglia and the implications for mental illness［J］.Psychopharmacology (Berl), 2016, 233(9):1637-1650.

［14］Gong Y, Tong L, Yang R, et al. Dynamic changes in hippocampal microglia contribute to depressive-like behavior induced by early social isolation［J］. Neuropharmacology, 2018, 135:223-233.

［15］Xu H, Rajsombath MM, Weikop P, et al. Enriched environment enhances beta-adrenergic signaling to prevent microglia inflammation by amyloidbeta［J］. EMBO Mol Med, 2018, 10(9):e8931.

［16］Cao M, Hu PP, Zhang YL, et al. Enriched physical environment reverses spatial cognitive impairment of socially isolated APPswe/PS1dE9 transgenic mice before amyloidosis onset［J］. CNS Neurosci Ther, 2018, 24(3):202-211.

［17］Frank MG, Baratta MV, Sprunger DB, et al. Microglia serve as a neuroimmune substrate for stress-induced potentiation of CNS pro-inflammatory cytokine responses［J］. Brain Behav Immun, 2007, 21(1):47-59.

［18］Patil T, More Ⅴ, Rane D, et al. Pro-inflammatory cytokine Interleukin-1beta (IL-1beta) controls Leishmania infection［J］. Cytokine, 2018, 112:27-31.

［19］RossettiⅠ, Zambusi L, Finardi A, et al. Calcitonin gene-related peptide decreases IL-1beta, IL-6 as well as Ym1, Arg1, CD163 expression in a brain tissue context-dependent manner while ameliorating experimental autoimmune encephalomyelitis［J］. J Neuroimmunol, 2018, 323:94-104.

［20］Mitchell SJ, Bernier M, Mattison JA, et al. Daily fasting improves health and survival in male mice independent of diet composition and calories［J］. Cell Metab, 2019, 29(1):221-228.

［21］Hoshino S, Kobayashi M, Higami Y. Mechanisms of the anti-aging and prolongevity effects of caloric restriction: evidence from studies of genetically modified animals［J］. Aging (Albany NY), 2018, 10(9):2243-2251.

［22］Ma L, Wang R, Dong W, et al. Caloric restriction can improve learning and memory in C57/BL mice probably via regulation of the AMPK signaling pathway［J］. Exp Gerontol, 2018, 102:28-35.

［23］Qiu G, Liu S, So KF. Dietary restriction and brain health［J］. Neurosci Bull, 2010, 26(1):55-65.