Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation

LIU Ying  LI Qiu-yan  PENG Yao  YU Hai-tao*  ZHANG Chun-jing*

doi:10.16098/j.issn.0529-1356.2019.03.006

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (3) : 297-303. DOI: 10.16098/j.issn.0529-1356.2019.03.006

Cell and Molecules Biology

Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation

LIU Ying¹ LI Qiu-yan ^1,3 PENG Yao^1,3 YU Hai-tao ^2* ZHANG Chun-jing^1*

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Abstract

Objective The effect of uncoupling protein 2(UCP2) on the Fadu cells of the hypopharyngeal cancer was investigated in this study. The siRNA technology was used to study the effect of UCP2 on cell cycle and cell proliferation. Meanwhile, cisplatin, a commonly chemotherapeutic drug, was researched on the effect of proliferation in Fadu cells after down-regulated UCP2 gene. Methods Flow cytometry/PI staining was used to observe the effect of UCP2 on Fadu cell cycle. Clone formation assay was used to detect the effect of down-regulated UCP2 on the proliferation of Fadu cells. MTT assay and Brud assay were used to determine whether UCP2 enhanced the sensitivity of Fadu cells to cisplatin and to detect the effect of UCP2 on the proliferation of Fadu. Results The present result showed that down-regulated UCP2 expression inhibited Fadu cell cycle in G₁/S phase and cell colony formation, but had no synergistic effect on inhibition of Fadu proliferation by cisplatin; The expression of UCP2 was significantly inhibited by genipin, and then inhibited cell proliferation in a time-concentration-dependent manner. Conclusion The proliferation of Fadu cells was weakened and the cell cycle was inhibited in G₁/S phase after down-regulated UCP2, suggesting that UCP2 may play an important adjust role in the development in Fadu of hypopharyngeal cancer.

Key words

Hypopharyngeal cancer / Uncoupling protein 2 / Cisplatin / Proliferation / siRNA

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LIU Ying LI Qiu-yan PENG Yao YU Hai-tao ZHANG Chun-jing. Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation[J]. Acta Anatomica Sinica. 2019, 50(3): 297-303 https://doi.org/10.16098/j.issn.0529-1356.2019.03.006

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