Objective To effectively enhance the scalpel holding training, reduce the environmental requirements for training, and change the current experimental-teaching method . Methods We proposed a design method for intelligent scalpel holding training model, and corrected the design and experiment of version 2. Results The designed training model not only simulated anatomical work scene, but also intelligently recognized the hand-type of holding scalpel and the scalpel knife action. Concretely, the proposed model was able to apperceive the scalpel holding angle, force, insertion and evulsion, the direction, speed and distance of scalpel holding training. By using the four scalpel holding training plans, the proposed model completed the whole experimental teaching process, including scalpel holding training, interactive voice guidance and evaluation of the training result. Conclusion The experimental result shows that the teaching guidance model can greatly enhance the scalpel holding training teaching effect and efficiency, and is a worthy promotion of medical experimental teaching aids and training equipment.