Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty

ZOU Zhi-wei ZHANG Qian DONG Jian-jun*

doi:10.16098/j.issn.0529-1356.2019.01.022

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (1) : 128-131. DOI: 10.16098/j.issn.0529-1356.2019.01.022

Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty

ZOU Zhi-wei ZHANG Qian DONG Jian-jun*

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Abstract

Objective To establish an animal model of restenosis in diabetic lower extremity arterial disease rat after percutaneous transluminal angioplasty(PTA). Methods We divided diabetic rats(n=30) into three groups: atherosclerosis group(AS), restenosis group(RS) and control group. We examined the artery by hematoxylin and eosin(HE), and detected the plaque components by αsmooth muscle actin(α-SMA) immunohistochemical staining. Results Artery restenosis rat models were successfully developed. The smooth muscle cells (SMCs) were the key components of the RS plaque. Conclusion An experimental animal model of restenosis in diabetic lower extremity arterial disease rat after PTA is set up by the steps described in this study.

Key words

Diabetes / Restenosis / Vascular smooth muscle cell / Percutaneous transluminal angioplasty / HE staining / Immunohistochemistry / Rat

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ZOU Zhi-wei ZHANG Qian DONG Jian-jun. Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty[J]. Acta Anatomica Sinica. 2019, 50(1): 128-131 https://doi.org/10.16098/j.issn.0529-1356.2019.01.022

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