Objective To study the effect of Survivin shRNA interference on c-myc and NF-κB expression levels in esophageal cells ECA109. Methods Survivin shRNA interference technology was applied to liposomes transfection esophageal ECA109 cells. Half quantitative RT-PCR and Western blotting method were applied to detect genes expression of c-myc and NF-κB gene. Flow cytometry was used to detect the proliferation and apoptosis of cell lines index changes. Results 1. After Survivin shRNA transfected esophageal ECA109 cells, Survivin mRNA and protein expression obviously down-regulated, and c-myc mRNA and protein expression also down-regulated; 2. After Survivin down-regulated, NF-κB mRNA expression had no obvious change, however the phosphorylated proteins level of NF-κB was reduced, whose upstream factor nuclear factor kappa B inhibitor kinase predominate(IKK) alpha, IKK beta mRNA express was cut; 3. Esophageal cell ECA109 apoptosis increased significantly. Cells ratio increased in G₂ phase but decreased in S phase. The cell cycle blocked. Conclusion Survivin is effective on adjusting c-myc gene expression on mRNA and protein level. Survivin regulates NF-κB activation by down-regulation of IKK alpha, IKK beta mRNA expression.