Apoptosis of olfactory bulb cells in MPTP cynomolgus monkey model of Parkinson&rsquo;s dise&nbsp;

CHEN Ming-yu CHENG Wei YAN Li-li GUAN Yun-qian WU Di YUE Feng REN Zhen-hua*

doi:10.16098/j.issn.0529-1356.2019.01.001

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Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (1) : 3-7. DOI: 10.16098/j.issn.0529-1356.2019.01.001

Neurobiology

Apoptosis of olfactory bulb cells in MPTP cynomolgus monkey model of Parkinson’s dise

CHEN Ming-yu¹ CHENG Wei¹ YAN Li-li¹ GUAN Yun-qian² WU Di² YUE Feng³ REN Zhen-hua ^1,2*

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Abstract

Objective To explore the apoptosis and the proliferation of glia cells in olfactory bulb of cynomolgus monkeys damaged by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Methods Three adult cynomolgus monkeys were injected with MPTP to induce the damage of olfactory bulb as MPTP group (model group) and other three adult cynomolgus monkeys as saline group (control group). Immunohistochemical staining was performed to examine the expression of Caspase-3, Bcl-2, ionized calcium binding adaptor molecule-1(Iba-1) and glial fibrillary acidic protein(GFAP) in the olfactory bulb. The numbers of Caspase-3, Bcl-2, Iba1 and GFAP-positive cells were counted and the average absorbance was measured by Image J v1.8.0 in control and MPTP group. Results Compared with the normal control group, Caspase-3-positive cells increased significantly in the glomerular layer (GL) of olfactory bulb, and Bcl-2-positive cells decreased significantly in MPTP group after MPTP injury, and GFAP-positive and Iba-1-positive cells were located in GL and external plexiform layers (EPL). The number of GFAP-positive and Iba-1-positive cells increased in olfactory bulb in the MPTP group. Conclusion MPTP can induce the cell apoptosis, astrocyte and microglial activation in olfactory bulb of cynomolgus monkeys, which may be associated with the dysosmia in Parkinson’s disease.

Key words

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Olfactory bulb / Immunohistochemistry / Cynomolgus monkey

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CHEN Ming-yu CHENG Wei YAN Li-li GUAN Yun-qian WU Di YUE Feng REN Zhen-hua. Apoptosis of olfactory bulb cells in MPTP cynomolgus monkey model of Parkinson’s dise [J]. Acta Anatomica Sinica. 2019, 50(1): 3-7 https://doi.org/10.16098/j.issn.0529-1356.2019.01.001

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